nf 4831 e nf 4832

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<p>3M Food Safety Appendix - Flow diagram of the reference methods: NF ISO 4831 (2006) and NF ISO 4832 (2006) NF ISO 4831 (2006) - Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of coliforms - Most probable number technique Initial suspension (solid product) Liquid sample xg + 9 X ml of appropriate diluents according to ISO 6887 parts 10 ml X 3 tubes 1 ml X 3 tubes Lauryl sulfate Initial suspension tryptose broth (LST) and decimal dilutions Lauryl sulfate Double concentration tryptose broth (LST) Simple concentration Incubation for Incubation for 24 h 2 h at 1°C 24 h to 48 h + 2 h at 30°C 1°C Transfer 10 of each positive LST broth into a BLBVB tube Incubation for 24 h to 48 h at 30°C 1°C Count the number of positive BLBVB tubes (with gas) per dilution Calculation taking into account the MPN table NF ISO 4832 (2006) Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of - Colony-count technique X g + 9x ml appropriate diluent according to ISO 6887 parts Decimal dilution in peptone-salt 1 ml per plate Add VRBL (15 ml) + double layer (4 ml) Incubation 24 h + 2 h at 30°C 1°C Characteristic colony enumeration ADRIA Développement 54/105 29 October 2021 Summary report (Version 0) 3M Petrifilm RCC</p>