LIVRO Plant Ecology
692 pág.

LIVRO Plant Ecology


DisciplinaBotânica3.377 materiais18.241 seguidores
Pré-visualização50 páginas
of stress After 48 hours incubation
Leaf pieces
Petri dish with moist filter paper, 5 -8 °C
Necrotic areas
H
+
, Cl
\u2013
HClN
H3C
H3C H Cl
CH3
NH2 N
H3C
H3C
CH3
NH2
Neutral red solution
Thin sections
of stressed
(e.g. frozen and
thawed) tissue
Dead cells
Intact cells
N
N
N
N
pH 6.8: red pH 8: yellow
Before stress application
Cell sap (vacuole)
stained with anthocyanin
Cell which cannot be
plasmolysed: killed
Cell nucleus Cell which can be
plasmolysed: living
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µ
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2015105
Time (min)
Killed tissue
Partially damaged
ControlIons from
cut cells
Battery µVoltmeter
Distilled
water
Leaf pieces
Magnetic stirrer
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