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of stress After 48 hours incubation Leaf pieces Petri dish with moist filter paper, 5 -8 °C Necrotic areas H + , Cl \u2013 HClN H3C H3C H Cl CH3 NH2 N H3C H3C CH3 NH2 Neutral red solution Thin sections of stressed (e.g. frozen and thawed) tissue Dead cells Intact cells N N N N pH 6.8: red pH 8: yellow Before stress application Cell sap (vacuole) stained with anthocyanin Cell which cannot be plasmolysed: killed Cell nucleus Cell which can be plasmolysed: living , \ufffd\ufffd\ufffd ,\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd \ufffd\ufffd - \ufffd\ufffd \ufffd\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd - \ufffd\ufffd!, \ufffd\ufffd\ufffd\ufffd\ufffd" \ufffd\ufffd\ufffd\ufffd \ufffd\ufffd \ufffd \ufffd\ufffd\ufffd \ufffd \ufffd\ufffd\ufffd \ufffd\ufffd" \ufffd\ufffd + \ufffd\ufffd\ufffd,\ufffd"\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd J\ufffd>\ufffd> \ufffd\ufffd\ufffd \ufffd\ufffd\ufffd %\ufffd\ufffd\ufffdK \ufffd \ufffd\ufffd\ufffd \ufffd\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd\ufffd \ufffd , \ufffd\ufffd\ufffd\ufffd \ufffd\ufffd! \ufffd\ufffd\ufffd\ufffd\ufffd \ufffd\ufffd!\ufffd" \ufffd\ufffd \ufffd \ufffd\ufffd \ufffd \ufffd !\ufffd=\ufffd\ufffd ,\ufffd\ufffd% \ufffd! 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