<div id="pf1" class="pf w0 h0" data-page-no="1"><div class="pc pc1 w0 h0"><img class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg1.png"><div class="t m0 x1 h1 y1 ff1 fs0 fc0 sc0 ls0 ws0">Replicação do DN<span class="_0 blank"></span>A </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf2" class="pf w0 h0" data-page-no="2"><div class="pc pc2 w0 h0"><img class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg2.png"><div class="t m0 x2 h2 y2 ff2 fs1 fc0 sc0 ls1">-<span class="ff3 ls0 ws0">Watson & Crick (1953): </span></div><div class="t m0 x3 h3 y3 ff3 fs2 fc0 sc0 ls2 ws0"> <span class="ff4 fs1 ls3 v1">\u201c<span class="ff5 ls0">Não escapou à noss<span class="_0 blank"></span>a atenção que o pareamento </span></span></div><div class="t m0 x4 h4 y4 ff5 fs1 fc0 sc0 ls0 ws0">específico por nós post<span class="_0 blank"></span>ulado imediatamente sugere um </div><div class="t m0 x5 h4 y5 ff6 fs1 fc0 sc0 ls0 ws0">possível <span class="_0 blank"></span>mecanismo de cópia do material genético\u201d.<span class="_1 blank"></span><span class="ff3"> </span></div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf3" class="pf w0 h0" data-page-no="3"><div class="pc pc3 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg3.png"><div class="t m0 x6 h5 y6 ff3 fs3 fc1 sc0 ls0 ws0">Características da Replicação: </div><div class="t m0 x6 h5 y7 ff3 fs3 fc1 sc0 ls0 ws0"> </div><div class="t m0 x6 h5 y8 ff3 fs3 fc1 sc0 ls9 ws1">1. <span class="ls0 ws0">Semi-conservativa </span></div><div class="t m0 x7 h6 y9 ff3 fs4 fc0 sc0 ls0 ws0">M. <span class="_0 blank"></span>S. Meselson & F. <span class="_0 blank"></span>W. Stahl (1958) </div><div class="t m0 x7 h6 ya ff3 fs4 fc0 sc0 ls0 ws0"> </div><div class="t m0 x7 h7 yb ff3 fs5 fc0 sc0 ls0 ws0">- Uso <span class="_0 blank"></span>de isótopos: <span class="_0 blank"></span>N<span class="fs6 ls4 v2">14</span> <span class="_0 blank"></span>e N<span class="fs6 ls4 v2">15</span><span class="lsa">; <span class="_0 blank"></span><span class="ls0"> </span></span></div><div class="t m0 x7 h7 yc ff2 fs5 fc0 sc0 ls5">-<span class="ff3 ls0 ws0"> Cres<span class="_0 blank"></span>cimento <span class="_0 blank"></span>por 14 gerações; </span></div><div class="t m0 x7 h8 yd ff2 fs7 fc0 sc0 ls6">-<span class="ff3 ls0 ws0">Centrifugação <span class="_2 blank"></span>em gradien<span class="_0 blank"></span>te <span class="_0 blank"></span>de </span></div><div class="t m0 x2 h7 ye ff3 fs5 fc0 sc0 ls0 ws0">densidade </div><div class="t m0 x7 h7 yf ff2 fs5 fc0 sc0 ls5">-<span class="ff3 ls0 ws0"> Bande<span class="_0 blank"></span>amento em <span class="_2 blank"></span>gradiente.<span class="fc2"> </span></span></div><div class="t m0 x4 h6 y10 ff2 fs4 fc0 sc0 ls7">-<span class="ff3 ls0 ws0"> N<span class="fs8 ws2 v3">14</span> N<span class="fs8 ws2 v3">14</span>: banda <span class="_0 blank"></span>leve. </span></div><div class="t m0 x4 h6 y11 ff2 fs4 fc0 sc0 ls7">-<span class="ff3 ls8">N<span class="fs8 ls0 ws2 v3">15</span><span class="ls0 ws0"> N<span class="fs8 ws2 v3">15</span>: banda pesada<span class="_0 blank"></span>. </span></span></div><div class="t m0 x4 h6 y12 ff2 fs4 fc0 sc0 ls7">-<span class="ff3 ls0 ws0"> N<span class="fs8 ws2 v3">14</span> N<span class="fs8 ws2 v3">15</span>: banda <span class="_0 blank"></span>intermediária. </span></div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf4" class="pf w0 h0" data-page-no="4"><div class="pc pc4 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg4.png"></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf5" class="pf w0 h0" data-page-no="5"><div class="pc pc5 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg5.png"><div class="t m0 x6 h5 y6 ff3 fs3 fc1 sc0 ls0 ws0">Características da Replicação: </div><div class="t m0 x6 h5 y7 ff3 fs3 fc1 sc0 ls0 ws0"> </div><div class="t m0 x6 h5 y8 ff3 fs3 fc1 sc0 ls0 ws0">2. Em fragmentos </div><div class="t m0 x6 h5 y13 ff3 fs3 fc0 sc0 ls0 ws0"> </div><div class="t m0 x7 h9 y14 ff3 fs5 fc0 sc0 ls0 ws0">Reiji <span class="_0 blank"></span>Okasaki e <span class="_0 blank"></span>colaboradores <span class="_0 blank"></span>(1960)<span class="ff5"> </span></div><div class="t m0 x8 h7 y15 ff7 fs5 fc0 sc0 lsb">\u2022<span class="ff3 ls0 ws0">DNA: <span class="_0 blank"></span>fitas <span class="_0 blank"></span>anti-paralelas </span></div><div class="t m0 x8 h7 y16 ff7 fs5 fc0 sc0 lsb">\u2022<span class="ff3 ls0 ws0">Fita <span class="_0 blank"></span>líder: síntes<span class="_0 blank"></span>e contínua </span></div><div class="t m0 x8 h7 y17 ff7 fs5 fc0 sc0 lsb">\u2022<span class="ff3 ls0 ws0">Fita <span class="_0 blank"></span>atrasada: síntes<span class="_0 blank"></span>e </span></div><div class="t m0 x8 h7 y18 ff3 fs5 fc0 sc0 ls0 ws0">descontínua </div><div class="t m0 x8 h7 y19 ff7 fs5 fc0 sc0 lsb">\u2022<span class="ff3 ls0 ws0">Fragmentos <span class="_0 blank"></span>de <span class="_0 blank"></span>Okasaki: </span></div><div class="t m0 x9 h7 y1a ff3 fs5 fc0 sc0 ls0 ws0">- Bactérias: <span class="_2 blank"></span>1000<span class="_1 blank"> </span> a 2000 nuc </div><div class="t m0 x9 h7 y1b ff3 fs5 fc0 sc0 ls0 ws0">- Eucariotos: <span class="_2 blank"></span>100 a 200 nuc </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf6" class="pf w0 h0" data-page-no="6"><div class="pc pc6 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg6.png"><div class="t m0 x2 h7 y1c ff3 fs5 fc0 sc0 ls0 ws0">A síntes<span class="_0 blank"></span>e do <span class="_0 blank"></span>DNA <span class="_0 blank"></span>é semi-descontínua <span class="_0 blank"></span>e <span class="_0 blank"></span>requer <span class="_0 blank"></span>um RNA <span class="_0 blank"></span>iniciador </div><div class="t m0 xa ha y1d ff8 fs9 fc1 sc0 ls0 ws0">Fita contínua </div><div class="t m0 xa hb y1e ff8 fsa fc1 sc0 ls0 ws0">Fita des<span class="_0 blank"></span>contínua </div><div class="t m0 xb ha y1f ff8 fs9 fc1 sc0 ls0 ws0">Síntese da Fita descontínua </div><div class="t m0 xc ha y20 ff8 fs9 fc1 sc0 ls0 ws0">Síntese da Fita Contínua </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf7" class="pf w0 h0" data-page-no="7"><div class="pc pc7 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg7.png"><div class="t m0 x6 h5 y6 ff3 fs3 fc1 sc0 ls0 ws0">Características da Replicação: </div><div class="t m0 x6 h5 y7 ff3 fs3 fc1 sc0 ls0 ws0"> </div><div class="t m0 x6 h5 y8 ff3 fs3 fc1 sc0 ls0 ws0">3. Bidirecional </div><div class="t m0 x7 h7 y21 ff3 fs5 fc0 sc0 ls0 ws0">John <span class="_0 blank"></span>Cairns (1963) </div><div class="t m0 x7 h7 y22 ff3 fs5 fc0 sc0 ls0 ws0"> </div><div class="t m0 x7 hc y23 ff1 fsb fc0 sc0 ls0 ws0">- <span class="fs7">Duas <span class="_0 blank"></span>forquilhas <span class="_0 blank"></span>de replicação; <span class="_2 blank"></span> </span></div><div class="t m0 x7 hd y24 ff1 fs5 fc0 sc0 ls0 ws0">- Estrutura <span class="_2 blank"></span>em <span class="ff9 sc1 lsc">\uf071</span> </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf8" class="pf w0 h0" data-page-no="8"><div class="pc pc8 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg8.png"></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf9" class="pf w0 h0" data-page-no="9"><div class="pc pc9 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bg9.png"><div class="t m0 xd he y25 ff3 fsc fc0 sc0 ls0 ws0">Replicação <span class="_2 blank"></span>em procariotos </div><div class="t m0 xd hf y26 ff2 fsd fc0 sc0 lsd">-<span class="ff3 ls0 ws0">Preparação: <span class="_0 blank"></span>Topoisomerase <span class="_2 blank"></span>II<span class="_1 blank"></span> <span class="ff4 lse">\u2013</span> Girase </span></div><div class="t m0 xe hf y27 ff2 fsd fc0 sc0 lsf">-<span class="ff3 ls0 ws0">Imprime <span class="_0 blank"></span>super-helicoidizações <span class="_2 blank"></span>negativas<span class="_1 blank"></span> </span></div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pfa" class="pf w0 h0" data-page-no="a"><div class="pc pca w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/04842b9b-aff3-4d0c-846f-cddc21651a65/bga.png"><div class="t m0 xf he y28 ff3 fsc fc0 sc0 ls0 ws0">Replicação <span class="_2 blank"></span>em procariotos </div><div class="t m0 xf hf y29 ff3 fsd fc0 sc0 ls0 ws0">-Iniciação: </div><div class="t m0 x6 h10 y2a ff3 fse fc0 sc0 ls0 ws0">- Origem:<span class="_0 blank"></span> Ori <span class="_1 blank"> </span>C; </div><div class="t m0 x6 h10 y2b ff3 fse fc0 sc0 ls0 ws0">- 245 <span class="_0 blank"></span>pb altamente conservados; </div><div class="t m0 x6 h10 y2c ff3 fse fc0 sc0 ls0 ws0">- Duas sequências-chave: </div><div class="t m0 x6 h10 y2d ff3 fse fc0 sc0 ls0 ws0">3 repetiçõe<span class="_0 blank"></span>s de 13 pb ricos em AT; </div><div class="t m0 x6 h10 y2e ff3 fse fc0 sc0 ls0 ws0">4 repetiçõe<span class="_0 blank"></span>s de 9 pb. </div><div class="t m0 x10 hd y2f ff1 fs5 fc0 sc0 ls0 ws0">Enzimas: </div><div class="t m0 x10 hc y30 ff1 fs7 fc0 sc0 ls0 ws0">1. <span class="_0 blank"></span>Proteína <span class="_0 blank"></span>iniciadora <span class="_2 blank"></span><span class="ffa ls10">\u2013<span class="ff1 ls0"> Dna <span class="_2 blank"></span>A </span></span></div><div class="t m0 x10 hd y31 ff1 fs5 fc0 sc0 ls0 ws0">2. <span class="_0 blank"></span>Helicase <span class="ffa ls11">\u2013</span> <span class="_0 blank"></span>Dna B </div><div class="t m0 x10 hd y32 ff1 fs5 fc0 sc0 ls0 ws0">3. <span class="_0 blank"></span>Inibidor da <span class="_0 blank"></span>helicase <span class="_0 blank"></span><span class="ffa ls11">\u2013<span class="ff1 ls0"> Dna C </span></span></div><div class="t m0 x10 hd y33 ff1 fs5 fc0 sc0 ls0 ws0">4. <span class="_0 blank"></span>SSB </div><div class="t m0 x10 hd y34 ff1 fs5 fc0 sc0 ls0 ws0">5. <span class="_0 blank"></span>Primase <span class="ffa ls11">\u2013</span> <span class="_0 blank"></span>Dna G <span class="_0 blank"></span>(RNA<span class="_2 blank"></span> pol.) </div><div class="t m0 x10 hc y35 ff1 fs7 fc0 sc0 ls0 ws0"> </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div>
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