Joseph J. Stephanos, Anthony W. Addison Chemistry of metalloproteins  problems and solutions in bioinorganic chemistry(1)(1)
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Joseph J. Stephanos, Anthony W. Addison Chemistry of metalloproteins problems and solutions in bioinorganic chemistry(1)(1)

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Chemistry of Metalloproteins: Problems and Solutions in Bioinorganic Chemistry \u2022
Joseph J. Stephanos and Anthony W. Addison
Problems and Solutions in
Bioinorganic Chemistry
Copyright \ue002 2014 by John Wiley & Sons, Inc. All rights reserved
Published by John Wiley & Sons, Inc., Hoboken, New Jersey
Published simultaneously in Canada
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Library of Congress Cataloging-in-Publication Data:
Stephanos, Joseph J., author.
Chemistry of metalloproteins: problems and solutions in bioinorganic chemistry/by Joseph J.
Stephanos, Anthony W. Addison.
p.; cm. \u2013 (Wiley series in protein and peptide science)
Includes bibliographical references and index.
ISBN 978-1-118-47044-2 (paperback)
I. Addison, A. W., author. II. Title. III. Series: Wiley series in protein and peptide science.
[DNLM: 1. Metalloproteins\u2013chemistry\u2013Examination Questions. QU 18.2]
Printed in the United States of America
10 9 8 7 6 5 4 3 2 1
Preface ix
1 Introduction 1
Proteins: Formation, Structures, and Metalloproteins, 4
References, 28
2 Alkali and Alkaline Earth Cations 31
References, 67
3 Nonredox Metalloenzymes 71
Carboxypeptidases, 75
Carbonic Anhydrase, 84
Alcohol Dehydrogenase, 88
References, 91
4 Copper Proteins 95
Introduction, 95
Electronic Spectra of Copper Ions, 96
ESR Spectra of Copper Ions, 105
Copper Proteins, 117
Plastocyanin, 119
Azurin and Stellacyanin, 127
Superoxide Dismutase, 131
Hemocyanin, 135
Ascorbic Oxidase, 139
References, 142
5 Iron Proteins 147
Introduction, 147
Electronic Spectra of Iron Ions, 148
Mössbauer Spectroscopy of Iron Ions, 155
ESR Spectra of Iron (III), 161
Iron Bioavailability, 166
Siderophores, 171
Iron Storage and Transfer Proteins, 184
Ferritin, 184
Transferrin, 187
Dioxygenase Iron Proteins, 195
Iron\u2013Sulfur Proteins, 207
Rubredoxin, 207
Ferredoxins, 212
2Fe\u20132S Ferredoxins, 212
4Fe\u20134S Ferredoxins, 221
Aconitase, 226
Hydroxylases, 229
Hydrogenases, 232
Nitrogenases, 240
Binuclear Iron Proteins, 251
Hemerythrin, 253
Ribotide Reductase, Purple Acid Phosphate, and
Methane Monooxygenase, 260
Hemoproteins: Classi\ufb01cation and Behavior of Heme in
Absence of Globins, 267
Myoglobin and Hemoglobin, 275
Myoglobin, 275
Hemoglobin, 280
Cytochrome C, 298
Electron Transfer in Porphyrins and Metalloporphyrins, 301
Catalases, 311
Peroxidases, 315
Cytochrome P-450, 322
Electronic Spectra of Hemoproteins, 327
ESR Spectra of Hemoproteins, 362
References, 378
6 Vitamin B12 393
References, 405
7 Chlorophyll 407
References, 421
Index 423
This book is an attempt to reveal the chemical concepts that rule the biological action
of metalloproteins. The emphasis is on building up an understanding of basic ideas
and familiarization with basic techniques. Enough background information is pro-
vided to introduce the \ufb01eld from both chemical and biological areas. It is hoped that
the book may be of interest to workers in biological sciences, and so, primarily for this
purpose, a brief survey of relevant properties of transition metals is presented.
The book is intended for undergraduates and postgraduates taking courses in
coordination chemistry and students in biology and medicine. It should also be a value
to research workers who would like an introduction to this area of inorganic
chemistry. It is very suitable for self-study; the range covered is so extensive that
the book can serve as a student\u2019s companion throughout his or her university career.
At the same time, teachers can turn to it for ideas and inspirations.
The book is divided into seven chapters and covers a full range of topics in
bioinorganic chemistry. It is well-illustrated and each chapter contains suggestions for
further reading, providing access to important review articles and papers of relevance.
A reference list is also included, so that the interested reader can readily consult the
literature cited in the text.
It is hoped that the present book will provide the basis for a more advanced study in
this \ufb01eld.
The discipline of bioinorganic chemistry is concerned with the function of metallic
and most of nonmetallic elements in biological processes. Also, it is the study of the
chemistry, structure, and reactions of the metalloprotein molecules belonging to the
living cell.
The precise concentrations of different ions, for instance, in blood plasma indicate
the importance of these ions for biological processes, (Table 1-1).
Such elements fall into four broad classi\ufb01cations: the polluting, contaminating,
bene\ufb01cial, and essential elements.
\u2022 Polluting elements: Pb, Hg, and Cd
\u2022 Contaminating elements: vary from person to person
\u2022 Bene\ufb01cial elements: Si, V, Cr, Se, Br, Sn, F, and Ni
\u2022 Essential elements: H, C, N, O, Na, Mg,