Joseph J. Stephanos, Anthony W. Addison Chemistry of metalloproteins  problems and solutions in bioinorganic chemistry(1)(1)
451 pág.

Joseph J. Stephanos, Anthony W. Addison Chemistry of metalloproteins problems and solutions in bioinorganic chemistry(1)(1)


DisciplinaQuímica Bioinorgânica6 materiais310 seguidores
Pré-visualização50 páginas
CHEMISTRY OF
METALLOPROTEINS
WILEY SERIES IN PROTEIN AND PEPTIDE SCIENCE
VLADIMIR N. UVERSKY, Series Editor
Metalloproteomics \u2022 Eugene A. Permyakov
Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and
Conformation \u2022 Vladimir Uversky and Sonia Longhi
Protein Misfolding Diseases: Current and Emerging Principles and Therapies \u2022
Marina Ramirez-Alvarado, Jeffery W. Kelly, and Christopher M. Dobson
Calcium Binding Proteins \u2022 Eugene A. Permyakov and Robert H. Kretsinger
Protein Chaperones and Protection from Neurodegenerative Diseases\u2022 Stephan Witt
Transmembrane Dynamics of Lipids \u2022 Philippe Devaux and Andreas Herrmann
Flexible Viruses: Structural Disorder in Viral Proteins \u2022 Vladimir Uversky and Sonia
Longhi
Protein Families: Relating Protein Sequence, Structure, and Function \u2022 Christine A.
Orengo and Alex Bateman
Chemistry of Metalloproteins: Problems and Solutions in Bioinorganic Chemistry \u2022
Joseph J. Stephanos and Anthony W. Addison
CHEMISTRY OF
METALLOPROTEINS
Problems and Solutions in
Bioinorganic Chemistry
JOSEPH J. STEPHANOS
ANTHONY W. ADDISON
Copyright \ue002 2014 by John Wiley & Sons, Inc. All rights reserved
Published by John Wiley & Sons, Inc., Hoboken, New Jersey
Published simultaneously in Canada
No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form
or by any means, electronic, mechanical, photocopying, recording, scanning, or otherwise, except as
permitted under Section 107 or 108 of the 1976 United States Copyright Act, without either the prior
written permission of the Publisher, or authorization through payment of the appropriate per-copy fee to
the Copyright Clearance Center, Inc., 222 Rosewood Drive, Danvers, MA 01923, (978) 750-8400,
fax (978) 750-4470, or on the web at www.copyright.com. Requests to the Publisher for permission
should be addressed to the Permissions Department, John Wiley & Sons, Inc., 111 River Street, Hoboken,
NJ 07030, (201) 748-6011, fax (201) 748-6008, or online at http://www.wiley.com/go/permission.
Limit of Liability/Disclaimer of Warranty: While the publisher and author have used their best efforts
in preparing this book, they make no representations or warranties with respect to the accuracy or
completeness of the contents of this book and speci\ufb01cally disclaim any implied warranties of
merchantability or \ufb01tness for a particular purpose. No warranty may be created or extended by sales
representatives or written sales materials. The advice and strategies contained herein may not be suitable
for your situation. You should consult with a professional where appropriate. Neither the publisher nor
author shall be liable for any loss of pro\ufb01t or any other commercial damages, including but not limited to
special, incidental, consequential, or other damages.
For general information on our other products and services or for technical support, please contact our
Customer Care Department within the United States at (800) 762-2974, outside the United States
at (317) 572-3993 or fax (317) 572-4002.
Wiley also publishes its books in a variety of electronic formats. Some content that appears in print may
not be available in electronic formats. For more information about Wiley products, visit our web site
at www.wiley.com.
Library of Congress Cataloging-in-Publication Data:
Stephanos, Joseph J., author.
Chemistry of metalloproteins: problems and solutions in bioinorganic chemistry/by Joseph J.
Stephanos, Anthony W. Addison.
p.; cm. \u2013 (Wiley series in protein and peptide science)
Includes bibliographical references and index.
ISBN 978-1-118-47044-2 (paperback)
I. Addison, A. W., author. II. Title. III. Series: Wiley series in protein and peptide science.
[DNLM: 1. Metalloproteins\u2013chemistry\u2013Examination Questions. QU 18.2]
QP551
5720.6076\u2013dc23
2013041995
Printed in the United States of America
10 9 8 7 6 5 4 3 2 1
CONTENTS
Preface ix
1 Introduction 1
Proteins: Formation, Structures, and Metalloproteins, 4
References, 28
2 Alkali and Alkaline Earth Cations 31
References, 67
3 Nonredox Metalloenzymes 71
Carboxypeptidases, 75
Carbonic Anhydrase, 84
Alcohol Dehydrogenase, 88
References, 91
4 Copper Proteins 95
Introduction, 95
Electronic Spectra of Copper Ions, 96
ESR Spectra of Copper Ions, 105
Copper Proteins, 117
Plastocyanin, 119
Azurin and Stellacyanin, 127
Superoxide Dismutase, 131
v
Hemocyanin, 135
Ascorbic Oxidase, 139
References, 142
5 Iron Proteins 147
Introduction, 147
Electronic Spectra of Iron Ions, 148
Mössbauer Spectroscopy of Iron Ions, 155
ESR Spectra of Iron (III), 161
Iron Bioavailability, 166
Siderophores, 171
Iron Storage and Transfer Proteins, 184
Ferritin, 184
Transferrin, 187
Dioxygenase Iron Proteins, 195
Iron\u2013Sulfur Proteins, 207
Rubredoxin, 207
Ferredoxins, 212
2Fe\u20132S Ferredoxins, 212
4Fe\u20134S Ferredoxins, 221
Aconitase, 226
Hydroxylases, 229
Hydrogenases, 232
Nitrogenases, 240
Binuclear Iron Proteins, 251
Hemerythrin, 253
Ribotide Reductase, Purple Acid Phosphate, and
Methane Monooxygenase, 260
Hemoproteins: Classi\ufb01cation and Behavior of Heme in
Absence of Globins, 267
Myoglobin and Hemoglobin, 275
Myoglobin, 275
Hemoglobin, 280
Cytochrome C, 298
Electron Transfer in Porphyrins and Metalloporphyrins, 301
Catalases, 311
Peroxidases, 315
Cytochrome P-450, 322
Electronic Spectra of Hemoproteins, 327
ESR Spectra of Hemoproteins, 362
References, 378
vi CONTENTS
6 Vitamin B12 393
References, 405
7 Chlorophyll 407
References, 421
Index 423
CONTENTS vii
PREFACE
This book is an attempt to reveal the chemical concepts that rule the biological action
of metalloproteins. The emphasis is on building up an understanding of basic ideas
and familiarization with basic techniques. Enough background information is pro-
vided to introduce the \ufb01eld from both chemical and biological areas. It is hoped that
the book may be of interest to workers in biological sciences, and so, primarily for this
purpose, a brief survey of relevant properties of transition metals is presented.
The book is intended for undergraduates and postgraduates taking courses in
coordination chemistry and students in biology and medicine. It should also be a value
to research workers who would like an introduction to this area of inorganic
chemistry. It is very suitable for self-study; the range covered is so extensive that
the book can serve as a student\u2019s companion throughout his or her university career.
At the same time, teachers can turn to it for ideas and inspirations.
The book is divided into seven chapters and covers a full range of topics in
bioinorganic chemistry. It is well-illustrated and each chapter contains suggestions for
further reading, providing access to important review articles and papers of relevance.
A reference list is also included, so that the interested reader can readily consult the
literature cited in the text.
It is hoped that the present book will provide the basis for a more advanced study in
this \ufb01eld.
JOSEPH J. STEPHANOS
ANTHONY W. ADDISON
ix
1
INTRODUCTION
The discipline of bioinorganic chemistry is concerned with the function of metallic
and most of nonmetallic elements in biological processes. Also, it is the study of the
chemistry, structure, and reactions of the metalloprotein molecules belonging to the
living cell.
The precise concentrations of different ions, for instance, in blood plasma indicate
the importance of these ions for biological processes, (Table 1-1).
Such elements fall into four broad classi\ufb01cations: the polluting, contaminating,
bene\ufb01cial, and essential elements.
\u2022 Polluting elements: Pb, Hg, and Cd
\u2022 Contaminating elements: vary from person to person
\u2022 Bene\ufb01cial elements: Si, V, Cr, Se, Br, Sn, F, and Ni
\u2022 Essential elements: H, C, N, O, Na, Mg,