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FLEXMAP 3D® Hardware
User Manual
Copyright Information
© Luminex Corporation, 2011. All rights reserved. No part of this publication may be
reproduced, transmitted, transcribed, or translated into any language or computer language,
in any form or by any means without prior express, written consent of Luminex Corporation.
 LUMINEX CORPORATION
12212 Technology Boulevard
Austin, Texas 78727-6115
U.S.A.
Voice: (512) 219-8020
Fax: (512) 219-5195
Luminex® FLEXMAP 3D® Hardware User Manual
PN 89-00002-00-187 Rev C
November 2011
 
MDCI Ltd.
Arundel House
1 Liverpool Gardens,
Worthing
West Sussex BN11 1SL
United Kingdom
Luminex Corporation (Luminex) reserves the right to modify its products and services at any
time. This guide is subject to change without notice. Although prepared to ensure accuracy,
Luminex assumes no liability for errors or omissions, or for any damages resulting from the
application or use of this information.
The following are trademarks of Luminex Corporation: Luminex®, xMAP®, xPONENT®,
FLEXMAP 3D®.
All other trademarks, including ProClin®, Cheminert®, Windows® Pentium® and Dell® are
trademarks of their respective companies.
 
i
Standard Terms and Conditions for Use of Instrument
Product
By opening the packaging containing this product ("Product") or by using such Product in any
manner, you are consenting and agreeing to be bound by the following terms and conditions.
You are also agreeing that the following terms and conditions constitute a legally valid and
binding contract that is enforceable against you. If you do not agree to all of the terms and
conditions set forth below, you must promptly return the Product for a full refund prior to using
them in any manner.
1. Acceptance
ALL SALES ARE SUBJECT TO AND EXPRESSLY CONDITIONED UPON THE TERMS
AND CONDITIONS CONTAINED HEREIN, AND UPON BUYER'S ASSENT THERETO. NO
VARIATION OF THESE TERMS AND CONDITIONS SHALL BE BINDING UPON LUMINEX
CORPORATION ("LUMINEX") UNLESS AGREED TO IN WRITING AND SIGNED BY AN
AUTHORIZED REPRESENTATIVE OF LUMINEX. For purposes of this agreement, "Seller"
shall mean either Luminex, if the Product is purchased directly from Luminex, or a Luminex
authorized reseller. Buyer, by accepting the Product shall be deemed to have assented to the
terms and conditions set forth herein, notwithstanding any terms contained in any prior or
later communications from Buyer and whether or not Seller shall specifically or expressly
object to any such terms.
2. Warranties
THIS WARRANTY IS APPLICABLE FOR PARTS AND SERVICE FOR LUMINEX
INSTRUMENTS PURCHASED DIRECTLY FROM LUMINEX TO BUYER AND ONLY TO
THE EXTENT SUCH INSTRUMENTS ARE LOCATED IN NORTH AMERICA AND THE
COUNTRIES THAT COMPRISE THE EUROPEAN UNION. LUMINEX MAKES NO
WARRANTY, EITHER EXPRESS OR IMPLIED, WITH RESPECT TO PRODUCTS SOLD,
DISTRIBUTED, LOCATED OR USED OUTSIDE OF NORTH AMERICA OR THE
COUNTRIES COMPRISING THE EUROPEAN UNION. PRODUCTS SOLD OUTSIDE OF
NORTH AMERICA OR THE COUNTRIES COMPRISING THE EUROPEAN UNION ARE
SOLD ONLY ON AN "AS IS, WHERE IS" BASIS. NOTWITHSTANDING THE FOREGOING,
LUMINEX SHALL PROVIDE BUYER A WARRANTY ON FIELD SERVICE PARTS
PROCURED FROM LUMINEX FOR MAINTENANCE OF LUMINEX INSTRUMENTS IN ALL
COUNTRIES IN THE WORLD AND PER THE TERMS AND CONDITIONS HEREIN. TO
THE EXTENT THAT THE FOREGOING DISCLAIMERS ARE INVALID OR
UNENFORCEABLE UNDER THE LAWS OF ANY JURISDICTION, THE WARRANTY,
DISCLAIMER, LIMITATION OF LIABILITY AND OTHER PROVISIONS SET FORTH BELOW
SHALL THEREUPON BE EFFECTIVE TO THE FULLEST EXTENT PERMITTED BY
APPLICABLE LAW.
Notwithstanding Buyer's acceptance thereof, if Product is purchased directly from Luminex,
Luminex warrants that for a period of twelve (12) months from date of delivery that the
Product shall conform in all material respects with the Product Specifications provided by
Luminex with the Product. The warranty provided herein specifically excludes any software or
FLEXMAP 3D® Hardware User Manual
ii
hardware not provided by Luminex. If Product is purchased from a Luminex authorized
reseller, any warranty obligations shall be provided in writing directly by such Luminex
authorized reseller to Buyer. THIS WARRANTY IS EXCLUSIVE AND LUMINEX MAKES NO
OTHER WARRANTY, EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION ANY
IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR
PURPOSE. Seller's warranties made in connection with this sale shall not be effective if
Seller has determined, in its sole discretion, that Buyer has misused the Product in any
manner, has failed to use the Product in accordance with industry standards or practices or
has failed to use the Product in accordance with instructions, if any, furnished by Seller.
BUYER'S EXCLUSIVE REMEDY WITH RESPECT TO PRODUCT PROVED TO SELLER'S
SATISFACTION TO BE DEFECTIVE OR NONCONFORMING SHALL BE REPAIR OR
REPLACEMENT OF SUCH PRODUCTS WITHOUT CHARGE OR REFUND OF THE
PURCHASE PRICE, IN SELLER'S SOLE DISCRETION, UPON THE RETURN OF SUCH
PRODUCTS IN ACCORDANCE WITH SELLER'S INSTRUCTIONS BELOW. NEITHER
SELLER NOR LUMINEX SHALL IN ANY EVENT BE LIABLE FOR INCIDENTAL,
CONSEQUENTIAL OR SPECIAL DAMAGES OF ANY KIND RESULTING FROM ANY USE
OR FAILURE OF THE PRODUCT, EVEN IF SELLER OR LUMINEX HAS BEEN ADVISED
OF THE POSSIBILITY OF SUCH DAMAGE INCLUDING, WITHOUT LIMITATION,
LIABILITY FOR LOSS OF WORK IN PROGRESS, DOWN TIME, LOSS OF REVENUE OR
PROFITS, FAILURE TO REALIZE SAVINGS, LOSS OF PRODUCTS OF BUYER OR
OTHER USE OR ANY LIABILITY OF BUYER TO A THIRD PARTY ON ACCOUNT OF
SUCH LOSS, OR FOR ANY LABOR OR ANY OTHER EXPENSE, DAMAGE OR LOSS
OCCASIONED BY SUCH PRODUCT INCLUDING PERSONAL INJURY OR PROPERTY
DAMAGE UNLESS SUCH PERSONAL INJURY OR PROPERTY DAMAGE IS CAUSED BY
SELLER'S GROSS NEGLIGENCE.
In the event that Product is located outside of North America or the European Union and fails
to conform to the warranty set forth herein, during the warranty period: (i) Buyer shall notify
Luminex in a timely manner in writing that such Product failed to conform and shall furnish a
detailed explanation of any alleged nonconformity; (ii) Buyer at it's expense will contract
either Luminex or a Luminex trained service engineer to assess the issue and identify the
defective FS-PART; and (ii) at Luminex's option and election, Buyer shall either return such
nonconforming Product to Luminex's manufacturing facility or destroy such Product and
provide Luminex with written certification of destruction. In the event that a FS-PART is
returned to Luminex's manufacturing facility, Luminex may analyze such FS-PART for
defects. In the event that Luminex determines that such FS-PART is not defective, the FS-
PART shall be shipped to Buyer then Buyer shall be responsible for the payment for such FS-
PART and related shipping charges. Furthermore, in the event that Luminex determines that
such FS-PART is defective then Luminex shall be responsible for the payment for such FS-
PART and related shipping charges. Except as expressly provided herein, Buyer shall not
have the right to return a Product to Luminex without Luminex's prior written consent.
3. Buyer's Use of Product
Buyer shall not use this Product for any commercial purpose, including without limitation
performance of testing services, unless expressly agreed to in writing by Luminex or as
specifically authorized by Luminex through a Luminex distributor. Buyer agrees that no rights
or licenses under Luminex's patents shall be implied from the sale of the Product, except as
expressly provided herein or as specifically agreed to in writing by Luminex, and Buyer does
not receive any right under Luminex's patent rights hereunder. Buyer acknowledges and
agrees that the Product are sold and licensed only for use with Luminex's laser based
fluorescent analytical test instrumentation. Buyer further acknowledges that, unless otherwise
iii
indicated on the Product label, the Product has not received approval from the United States
Food and Drug Administration or other federal, state or local regulatory agencies and have
not been testedby Seller or Luminex for safety or efficacy in food, drug, medical device,
cosmetic, commercial or any other use, unless otherwise stated in Seller's technical
specifications or material data sheets furnished to Buyer. Buyer expressly represents and
warrants to Seller that Buyer will use the Product in accordance with the Product label, if
applicable, and will properly test and use any Product in accordance with the practices of a
reasonable person who is an expert in the field and in strict compliance with the United
States Food and Drug Administration and all applicable domestic and international laws and
regulations, now and hereinafter enacted.
BUYER HEREBY GRANTS TO LUMINEX A NONEXCLUSIVE, WORLDWIDE,
UNRESTRICTED, ROYALTY-FREE, FULLY PAID-UP LICENSE, WITH THE RIGHT TO
GRANT AND AUTHORIZE SUBLICENSES, UNDER ANY AND ALL PATENT RIGHTS IN
INVENTIONS COMPRISING MODIFICATIONS, EXTENSIONS, OR ENHANCEMENTS
MADE BY BUYER TO THE PRODUCT OR TO THE MANUFACTURE OR USE OF THE
PRODUCT ("IMPROVEMENT PATENTS"), TO MAKE, HAVE MADE, USE, IMPORT,
OFFER FOR SALE OR SELL ANY AND ALL PRODUCT; EXPLOIT ANY AND ALL
METHODS OR PROCESSES; AND OTHERWISE EXPLOIT IMPROVEMENT PATENTS
FOR ALL PURPOSES. NOTWITHSTANDING THE FOREGOING, "IMPROVEMENT
PATENTS" SPECIFICALLY EXCLUDES PATENT CLAIMS CONCEIVED AND REDUCED
TO PRACTICE BY BUYER CONSISTING OF METHODS OF SAMPLE PREPARATION,
METHODS OF CONJUGATING PRODUCT TO ANALYTES, THE COMPOSITION OF
MATTER OF THE SPECIFIC CHEMISTRIES OF THE ASSAYS DEVELOPED BY BUYER
AND METHODS OF PERFORMING THE ASSAYS (I.E., THE PROTOCOL FOR THE
ASSAY).
Buyer has the responsibility and hereby expressly assumes the risk to verify the hazards and
to conduct any further research necessary to learn the hazards involved in using the Product.
Buyer also has the duty to warn Buyer's customers, employees, agents, assigns, officers,
successors and any auxiliary or third party personnel (such as freight handlers, etc.) of any
and all risks involved in using or handling the Product. Buyer agrees to comply with
instructions, if any, furnished by Seller or Luminex relating to the use of the Product and not
misuse the Product in any manner. Buyer shall not reverse engineer, decompile, disassemble
or modify the Product. Buyer acknowledges that Luminex retains ownership of all patents,
trademarks, trade secrets and other proprietary rights relating to or residing in the Product
and Buyer receives no rights to such intellectual property rights by virtue of its purchase of
Product other than as expressly set forth herein. Buyer shall have no right to use any
trademarks owned or licensed to Luminex without the express written permission of Luminex.
4. Buyer's Representations, Release and Indemnity
Buyer represents and warrants that it shall use the Product in accordance with Paragraph 2,
"Buyer's Use of Product," and that any such use of Product will not violate any law,
regulation, judicial order or injunction. Buyer agrees to release, discharge, disclaim and
renounce any and all claims, demands, actions, causes of action and/or suits in law or equity,
now existing or hereafter arising, whether known or unknown, against Seller and Luminex,
and their respective officers, directors, employees, agents, successors and assigns
(collectively the "Released Parties"), with respect to the use of the Product. Buyer agrees to
indemnify and hold harmless the Released Parties from and against any suits, losses, claims,
demands, liabilities, costs and expenses (including attorney, accounting, expert witness, and
consulting fees) that any of the Released Parties may sustain or incur as a result of any claim
against such Released Party based upon negligence, breach of warranty, strict liability in tort,
FLEXMAP 3D® Hardware User Manual
iv
contract or any other theory of law or equity arising out of, directly or indirectly, the use of the
Product or by reason of Buyer's failure to perform its obligations contained herein. Buyer shall
fully cooperate with the Released Parties in the investigation and determination of the cause
of any accident involving the Product which results in personal injury or property damage and
shall make available to the Released Parties all statements, reports, recordings and tests
made by Buyer or made available to Buyer by others.
5. Patent Disclaimer
Neither Seller nor Luminex warrants that the use or sale of the Product will not infringe the
claims of any United States or other patents covering the product itself or the use thereof in
combination with other products or in the operation of any process.
v
End-User License Agreement (EULA) for Luminex®
xPONENT® Software
This Luminex End-User License Agreement (“EULA”) is a legal agreement between you
(either an individual or a single entity, also referred herein as “you”) the end-user and
Luminex Corporation (“Luminex”) regarding the use of the xPONENT software product
provided to you above, which includes computer SOFTWARE and online or electronic
documentation and may include associated media and printed materials (if any)
(“SOFTWARE”). The terms also apply to any updates, supplements, web content or internet-
based services, such as remote access.
BY USING THE SOFTWARE, YOU ACCEPT THESE TERMS. IF YOU DO NOT ACCEPT
THEM, DO NOT USE THE SOFTWARE. INSTEAD, RETURN IT TO LUMINEX OR THE
LUMINEX AUTHORIZED THIRD PARTY FROM WHICH YOU PURCHASED THE
SOFTWARE FOR A REFUND OR CREDIT. IF YOU COMPLY WITH THESE LICENSE
TERMS, YOU HAVE THE RIGHTS TO USE THE SOFTWARE AS SPECIFICALLY SET
FORTH BELOW.
1. OVERVIEW. The SOFTWARE is protected by copyright laws and international copyright
treaties, as well as other intellectual property laws and treaties. The SOFTWARE is
licensed, not sold.
2. ADDITIONAL LICENSING REQUIREMENTS AND/OR USE RIGHTS.
a. Trial and Conversion. Some or all of the SOFTWARE may be licensed on a trial basis.
Your rights to use trial SOFTWARE are limited to the trial period. The trial SOFTWARE
and length of the trial period are set forth during the activation process. The
SOFTWARE may be used for evaluation purposes only during the trial period and not
for any commercial use, including without limitation to any diagnostic use. You may
have the option to convert your trial rights to perpetual rights. Conversion options will
be presented to you at the expiration of your trial period.
b. Activation. You can activate the SOFTWARE by obtaining a license key provided by
Luminex Technical Support at support@luminexcorp.com or 1-877-785-2323 or
1-512-381-4397.
c. Branding. You may only add additional branding or other graphics to SOFTWARE with
Luminex's express written consent.
d. Upgrades. You may only obtain updates or upgrades for the SOFTWARE from
Luminex Technical Support at orders@luminexcorp.com or authorized resellers. For
more information on obtaining updates from authorized resellers, see http://
www.luminexcorp.com.
FLEXMAP 3D® Hardware User Manual
vi
3. GRANT OF LICENSE. Subject to the terms and conditions of this EULA, Luminex hereby
grants to you a nonexclusive, nontransferable, nonassignable license (without right to
sublicense) under Luminex's copyrights and trade secrets to use the SOFTWARE on a
single computer running with a single unit of a specific model of Luminex instrument, as
such model is identified on the packaging included with the SOFTWARE. You may make
one (1) copy of the SOFTWARE for backup or archival purposes only. You may also
install the SOFTWARE on up to two (2) additional computers for purposes of performing
ancillary tasks (i.e. preparing templates/protocols, performing further analysis or re-
running previous data), provided such computers are at a single location and are NOT
connected with a Luminex instrument. In addition, You may purchase the right to use the
SOFTWARE on additional computers, as agreed to in writing with Luminex or its
authorized reseller, for purposes of performing ancillary tasks (i.e. preparing templates/
protocols, performing further analysis or re-running previous data), providedsuch
computers are at a single location and are NOT connected with a Luminex instrument.
Although no rights or licenses under any of Luminex's patents are granted by or shall be
implied from the license of the SOFTWARE or the sale of Luminex instrumentation to
you, the purchaser, you may obtain a license under Luminex's patents, if any, to use this
unit of Luminex instrumentation with fluorescently labeled microsphere beads authorized
by Luminex by purchasing such beads from Luminex or an authorized Luminex reseller.
4. RESTRICTIONS
• SOFTWARE must only be installed and operated on a single computer running with a
Luminex instrument, as set forth above.
• You may not use this SOFTWARE for any commercial purpose, including in the
performance of testing services, unless expressly agreed to in writing by Luminex or as
authorized in writing by Luminex through an authorized reseller of the SOFTWARE.
• You may only use the SOFTWARE with microspheres manufactured by Luminex or
with kits developed, manufactured and distributed by licensees authorized in writing by
Luminex.
• You must maintain all proprietary notices on all copies of the SOFTWARE.
• You may not distribute copies of the SOFTWARE to third parties.
• You may not reverse-engineer, decompile, disassemble, or otherwise attempt to derive
source code from the SOFTWARE.
• You may not copy (other than one backup or archival copy), distribute, sublicense,
rent, lease, transfer or grant any rights in or to all or any portion of the SOFTWARE.
• You must comply with all applicable laws regarding the use of the SOFTWARE.
• You may not modify or prepare derivative works of the SOFTWARE, including
modifying any branding or graphics.
• You may not use the SOFTWARE in a computer-based service business or publicly
display visual output of the SOFTWARE.
• You may not transmit the SOFTWARE over a network, by telephone, or electronically
by any means.
vii
5. TERM AND TERMINATION. Your rights under this EULA are effective until termination.
You may terminate this EULA at any time by destroying the SOFTWARE, including all
computer programs and documentation, and erasing any copies residing on your
computer equipment. Luminex may terminate this EULA upon thirty (30) days written
notice to you. Your rights under this EULA automatically terminate without further action
on the part of Luminex if you do not comply with any of the terms or conditions of this
EULA. Upon any termination of this EULA, you agree to destroy the SOFTWARE and
erase any copies residing on your computer equipment.
6. RIGHTS IN SOFTWARE. All rights and title in and to the SOFTWARE and any copies
thereof are owned by Luminex or its suppliers. This EULA is not a sale and does not
transfer to you any title or ownership interest in or to the SOFTWARE or any patent,
copyright, trade secret, trade name, trademark or other intellectual property right therein.
You shall not remove, alter, or obscure any proprietary notices contained on or within the
SOFTWARE and shall reproduce such notices on any back-up copy of the SOFTWARE.
All title and intellectual property rights in and to the content which may be accessed
through use of the SOFTWARE is the property of the respective content owner and may
be protected by applicable copyright or other intellectual property laws and treaties. This
EULA grants you no rights to use such content.
7. EXPORT RESTRICTIONS. You agree that you will not export or re-export the
SOFTWARE to any country, person, entity, or end-user subject to U.S.A. export
restrictions. You hereby warrant no state or federal agency has suspended, revoked, or
denied your export privileges.
8. NO WARRANTY. THE SOFTWARE IS LICENSED "AS IS." ANY USE OF THE
SOFTWARE IS AT YOUR OWN RISK. THE SOFTWARE IS PROVIDED FOR USE
ONLY WITH LUMINEX PRODUCTS. TO THE MAXIMUM EXTENT PERMITTED BY
APPLICABLE LAW, LUMINEX AND ITS SUPPLIERS DISCLAIM ALL WARRANTIES,
EITHER EXPRESS OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, IMPLIED
WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE,
AND NONINFRINGEMENT.
9. LIMITATION OF LIABILITY. IN NO EVENT SHALL LUMINEX OR ITS SUPPLIERS BE
LIABLE FOR ANY SPECIAL, INCIDENTAL, INDIRECT, OR CONSEQUENTIAL
DAMAGES WHATSOEVER (INCLUDING, WITHOUT LIMITATION, DAMAGES FOR
LOSS OF BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS
INFORMATION, OR ANY OTHER PECUNIARY LOSS) ARISING OUT OF THE USE OF
OR INABILITY TO USE THE SOFTWARE, EVEN IF LUMINEX HAS BEEN ADVISED OF
THE POSSIBILITY OF SUCH DAMAGES.
10. MISCELLANEOUS. This EULA is governed by the laws of the State of Texas, U.S.A.,
without reference to conflicts of laws principles. You shall not assign or sublicense or
otherwise transfer the rights or license granted hereunder, by agreement or by operation
of law, without the prior written consent of Luminex, and all assignments in violation of
this prohibition shall be null and void. This EULA is the complete and exclusive
agreement of Luminex and you and supersedes all other communications, oral or written,
relating to the subject matter hereof. No change to this EULA shall be valid unless in
writing and signed by the party against whom enforcement is sought. The waiver or
failure of Luminex or you to exercise in any respect any right or rights provided for herein
shall not be deemed a waiver of any further right hereunder. If any provision of this EULA
is held unenforceable, the remainder of this EULA will continue in full force and effect.
89-30000-00-254 Rev. B
FLEXMAP 3D® Hardware User Manual
viii
Table of Contents
Chapter 1 Safety ..................................................................................................................1
Intended Use ...............................................................................................................................................1
Warnings and Notes ....................................................................................................................................1
Symbols .......................................................................................................................................................2
Testing and Certification ..............................................................................................................................3
Safety Precautions .......................................................................................................................................3
General ..................................................................................................................................................4
Electromagnetic Compatibility ...............................................................................................................4
Lasers ....................................................................................................................................................4
Fluidics ..................................................................................................................................................5
Biological Samples ................................................................................................................................6
Mechanical Components .......................................................................................................................6
Swivel Base ...........................................................................................................................................6
Indicator Lights ......................................................................................................................................6
Electrical Components ..........................................................................................................................7
Heat .......................................................................................................................................................8
Decontamination Procedure ........................................................................................................................8Installation of Instrument ..............................................................................................................................8
Disposal of Instrument .................................................................................................................................9
Chapter 2 Technical Overview .........................................................................................11
How the FLEXMAP 3D System Operates .................................................................................................11
Subsystems ...............................................................................................................................................11
Electronics ...........................................................................................................................................12
Fluidics ................................................................................................................................................13
Mechanics ...........................................................................................................................................16
Optics ..................................................................................................................................................18
System Components .................................................................................................................................18
Luminex xPONENT® 4.0 Software ......................................................................................................18
Reagents .............................................................................................................................................18
Luminex® FLEXMAP 3D® Hardware ..................................................................................................19
Recommended Additional Equipment .......................................................................................................19
Uninterruptible Power Supply (UPS) or Surge Protector .....................................................................20
Printer ..................................................................................................................................................20
Barcode Labels ...................................................................................................................................20
Vortex ..................................................................................................................................................20
Bath Sonicator .....................................................................................................................................20
Specifications .............................................................................................................................................20
General ................................................................................................................................................20
Electronics ...........................................................................................................................................21
Optics ..................................................................................................................................................21
Fluidics ................................................................................................................................................22
Microspheres .......................................................................................................................................22
Microtiter Plates ...................................................................................................................................22
 
ix
Chapter 3 Maintenance and Cleaning .............................................................................23
General Maintenance Precautions ............................................................................................................ 23
Daily Maintenance .....................................................................................................................................23
Initializing the FLEXMAP 3D Instrument .............................................................................................24
Warming Up the FLEXMAP 3D Instrument .........................................................................................24
Maintaining Fluids ............................................................................................................................... 24
Shutting Down the FLEXMAP 3D Instrument ......................................................................................25
Weekly Maintenance ................................................................................................................................. 25
Running Weekly Maintenance .............................................................................................................25
Removing Clogs ..................................................................................................................................26
Cleaning the Sample Probe ................................................................................................................ 26
Calibrating the FLEXMAP 3D System .................................................................................................27
Visually Inspect the FLEXMAP 3D Instrument ....................................................................................27
Monthly Maintenance .................................................................................................................................27
Semi-annual Maintenance .........................................................................................................................28
Replacing the Syringe Teflon Seals ....................................................................................................28
Replacing the HEPA Air Filter .............................................................................................................29
Cleaning the Ventilation Filters ............................................................................................................30
...................................................................................................................................................................32
Storing the FLEXMAP 3D Instrument ........................................................................................................33
Storing the FLEXMAP 3D Instrument ..................................................................................................33
Preparing the FLEXMAP 3D Instrument for Use After Storage ...........................................................33
Replacing Fuses ........................................................................................................................................33
Maintenance Logs ..................................................................................................................................... 35
Short Term Maintenance - One Week .................................................................................................35
Long Term Maintenance - One Year ...................................................................................................35
Chapter 4 Troubleshooting ..............................................................................................37
Overview ....................................................................................................................................................37
Power Supply Problems ............................................................................................................................ 38
Communication Problems ..........................................................................................................................38Clogs ..........................................................................................................................................................39
Pressurization Problems ............................................................................................................................39
Fluid Leaks ................................................................................................................................................ 40
Sample Probe Problems ............................................................................................................................40
Calibration Problems ................................................................................................................................. 41
Verification Problems .................................................................................................................................42
Acquisition Problems .................................................................................................................................43
Bead Detail Irregularities ........................................................................................................................... 44
FLEXMAP 3D® Hardware User Manual
x
Become familiar with the information in this chapter before using the equipment. Do not
perform procedures on your system that are not specifically contained in this manual, unless
you are directed to do so by Luminex Technical Support.
Intended Use
The Luminex® FLEXMAP 3D® system is a multiplex test system intended to measure and sort
multiple signals generated in an assay from a sample. The system includes a signal reader
unit, raw data storage mechanisms, data acquisition software and software to process
detected signals.
The FLEXMAP 3D system is for research use only and is not for use in diagnostic procedures
within the United States. The system has not been cleared or approved for clinical use by the
US Food and Drug Administration (FDA). The FLEXMAP 3D system is CE marked for in vitro
diagnostic use within the European Union (EU).
Warnings and Notes
The following informational notes and warnings appear as necessary in this manual.
NOTE: This message is used to provide general helpful information. No
safety or performance issues are involved.
CAUTION: This message is used in cases where the hazard is minor or only
a potential hazard is present. Failure to comply with the caution
may result in hazardous conditions.
WARNING: This message is used in cases where danger to the operator or
to the performance of the instrument is present. Failure to
comply with the warning may result in incorrect performance,
instrument failure, invalid results, or hazard to the operator.
DANGER: This message is used in cases where significant risk of serious
injury or death is present.
Chapter 1: Safety
 
1
Symbols
You may encounter these symbols during the use and operation of the Luminex® FLEXMAP
3D® system. They represent warnings, conditions, identifications, instructions, and regulatory
agencies. Warning symbols are further explained in the Safety Precautions section.
Symbol Description Symbol Description Symbol Description
Puncture/Pinch
Point Warning
General
Warning,
Caution, Risk
of Danger
Warning,
Biological
Hazard
Hand Crush/
Cut/Force
From Above
Heat/Hot
Surface
Warning
Burn Hazard/
Hot Surface
Caution, Risk
of Electric
Shock
Warning, Laser
Beam
Consult
Instructions for
Use
Protective
ground
Alternating
current (AC)
Catalog
Number
Use By
Expiration Date
Temperature
Limitation
Waste
Electrical and
Electronic
Equipment
(WEEE)
Date of
Manufacture
Manufacturer European
Union
Conformity
Off (Power) On (Power) MET Mark
Serial Number Batch Code European
Representative
In vitro Medical
Diagnostic
Device 1
1 The FLEXMAP 3D system is for research use only and is not for use in diagnostic procedures within the
US. The system has not been cleared or approved for clinical use by the US FDA.
FLEXMAP 3D® Hardware User Manual
2
Testing and Certification
The FLEXMAP 3D system has been tested by MET.
FIGURE 1. MET Mark
In addition, Luminex FLEXMAP 3D complies with European Union (EU) safety requirements
and therefore may be marketed in the Europe Single Market. The following European Union
compliance label appears on the back of the FLEXMAP 3D instrument.
FIGURE 2. European Union Compliance Label
Safety Precautions
Read the following safety information before using the FLEXMAP 3D instrument. This
instrument contains electrical, mechanical, and laser components that, if handled improperly,
are potentially harmful. In addition, biological hazards may be present during system
operation. Therefore, Luminex recommends that all system users become familiar with the
specific safety advisories below in addition to adhering to standard laboratory safety
practices.
In any situation in which you encounter this symbol, consult this manual or other Luminex
documentation to determine the nature of the potential hazard and any necessary actions
you must take.
CAUTION: The protection provided by the equipment can be impaired or the
warranty voided if the Luminex FLEXMAP 3D system is used in
a manner not specified by the instructions or by Luminex
Corporation.
Safety
3
General
CAUTION: Keep access doors closed during normal operation.
Always observe standard laboratory safety practices.
Electromagnetic Compatibility
The FLEXMAP 3D system complies with the emission and immunity requirements described
in and . The electromagnetic environment should be evaluated prior to operation.
WARNING: Do not use this device in close proximity to sources of strong
electromagnetic radiation, for example, unshielded intentional
RF sources, as these may interfere with proper operation
WARNING: Always handle the FLEXMAP 3D system according to Luminex
instructions to avoid any possible interference from its
electromagnetic fields.
Lasers
The Luminex® FLEXMAP 3D® instrument is classified under FDA 21 CFR 1040.10 and
1040.11 as a Class I laser product consisting of two Class IIIb lasers within the instrument.
The accessory bar code reader is classified as Class II. In accordance with IEC 60825-1, the
instrument is classified as Class 1, containing two Class 3b lasers, and including an
accessory Class 2 bar code reader. The FLEXMAP 3D complies with IEC 60825-1 and 21
CFR 1040.10 and 1040.11 except for deviations pursuant to Laser Notice No. 50, dated June
24, 2007.
The following label appears on the back of the FLEXMAP 3D instrument.
FIGURE 3. Laser Warning Label
The following label appears above the laser apertures located inside the optics enclosure
inside the FLEXMAP 3D instrument.
FLEXMAP 3D® Hardware User Manual
4
FIGURE 4. Avoid Exposure Label
All Class 3b laser apertures are located within the FLEXMAP 3D instrument and are
contained within a protective housing, accessible only to trained field service technicians.
When performing routine maintenance, turn power to the system off and disconnect the
power cord.
DANGER: Do not, under any circumstances, remove the FLEXMAP 3D
instrument cover. Use of controls or adjustments or performance
of procedures other than those specified in this manual can result
in hazardous radiation exposure.
The bar code reader laser presents a potential hazard to eyesight.
DANGER: Do not stare into the barcode reader beam or shine it into other
people’s eyes.
Fluidics
This instrument contains fluids. In the event of a fluid leak, turn off all power to the system
and disconnect all power cords. The on/off switch is not a method of disconnection; the
power cord must be removed from the outlet. Contact Luminex Technical Support for further
information.
DANGER: Do not operate the instrument in the presence of leaking fluid.
Optimally, the waste line should be routed to a laboratory drain. If you use a waste container,
monitor waste fluid levels periodically. The volume of the waste container should be at least
aslarge as the volume of the sheath container. Do not allow the waste fluid container to
overflow. Empty the waste fluid container each time you replace or fill the sheath fluid
container. Place the waste fluid container at least three feet below the surface on which the
Luminex® FLEXMAP 3D® instrument rests. Do not place the waste fluid container on top of
the instrument. Do not move the waste line vertically while the FLEXMAP 3D instrument is
running, and make certain the waste container is properly vented. Contact Luminex Technical
Support before relocating the waste fluid container or rerouting the waste line.
CAUTION: Reagents may contain sodium azide as a preservative. Sodium
azide is toxic and may also react with lead and copper plumbing
to form highly explosive metal azides. On disposal, flush drains
with a generous amount of cold water to prevent azide build-up.
Consult the manual guideline “Safety Management No. CDC-22,
Decontamination of Laboratory Sink Drains to remove Azide
Safety
5
salts” (Centers for Disease Control, Atlanta, Georgia, April 30,
1976).
Biological Samples
Human and animal samples may contain biohazardous infectious agents. Where exposure
to potentially biohazardous materia exists, follow appropriate biosafety procedures and use
personal protective equipment (PPE). PPE includes gloves, gowns, laboratory coats, face
shields or mask and eye protection, respirators, and ventilation devices. Observe all local,
state, federal and country specific biohazard handling regulations when disposing of
biohazardous waste material.
Mechanical Components
The FLEXMAP 3D instrument has parts that move during operation. Risk of personal
injury is present. The moving parts present puncture, pinching, and hand-crushing
hazards. Keep your hands and fingers away from the XY slot, syringe pumps, and
sample probe during operation. The XY platform ejects without warning, especially
during multiplate batches. Observe all warnings and cautions. Keep the access doors
closed during normal operations.
Swivel Base
The optional swivel base available with the FLEXMAP 3D system includes moving parts
and possible pinch points. Those factors present the possibility of injury.
Make certain the swivel base rests securely on a stable surface, to avoid any possibility of
toppling.
Indicator Lights
The lights inside the front door of the Luminex® FLEXMAP 3D® instrument indicate the on/off
status of the system and are harmless. The blue light-emitting diodes (LEDs) do not emit light
in the UV spectrum.
FLEXMAP 3D® Hardware User Manual
6
Electrical Components
Do not perform any maintenance or cleaning of the electrical components in the system,
with the exception of replacing fuses.
The following fuse caution label appears on the back of the FLEXMAP 3D.
FIGURE 5. Fuse Caution Label
The following voltage label appears on the back of the FLEXMAP 3D instrument. It displays
the FLEXMAP 3D serial number, model number, power requirements, and manufacturer’s
information.
FIGURE 6. Serial Number and Voltage Label
Safety
7
Heat
The heater plate, used to warm the heater block of the XY platform, can be heated between
35°C and 60°C.
WARNING: The heater plate of the FLEXMAP 3D XY platform may be hot
and can cause personal injury if touched. Do not touch the
heater plate.
Decontamination Procedure
Occasions may arise when it becomes necessary to decontaminate the entire Luminex®
FLEXMAP 3D® instrument, for example, prior to shipping. If you must decontaminate the
instrument, sanitize the accessible surfaces and the internal fluidics system. This is
particularly important when biohazardous samples have been run.
WARNING: Wear appropriate personal protective equipment when handling
parts that come into contact with potentially biohazardous
samples.
To decontaminate the FLEXMAP 3D instrument:
1. Remove all specimens and all FLEXMAP 3D reagents.
2. Use the software to run a sanitize command with the diluted (10% to 20%) household
bleach solution. Leave distilled water and household bleach solution diluted to 10% to
20% in water in the system.
3. Use the software to run two wash commands with distilled water.
4. Turn off the power switch and unplug the cord from the outlet.
5. Wash all exterior surfaces with a mild detergent followed by the diluted (10% to 20%)
household bleach solution.
6. Open the front doors of the system. Clean all accessible surfaces with mild detergent
followed by the diluted (10% to 20%) household bleach solution.
Installation of Instrument
Luminex handles the packing, shipping, unpacking, and installation of the Luminex®
FLEXMAP 3D® system. Luminex recommends that users and laboratory personnel do not
uninstall, move, or install the system.
For site preparation and facilities requirements, see the section titled Installation.
FLEXMAP 3D® Hardware User Manual
8
FIGURE 7. Installed FLEXMAP 3D System
Disposal of Instrument
Within the European Union, the Waste Electrical and Electronic Equipment Directive
2002/96/EC requires that you properly dispose of electrical and electronic equipment
when it reaches its end of life.
If you are disposing of a Luminex® FLEXMAP 3D® instrument, decontaminate the system.
See the section titled “Decontamination Procedure”. Next, contact Luminex Technical
Support for a Return Material Authorization (RMA) number at +1-512-381-4397 (outside of
the U.S.). Return the equipment to the following Luminex location:
Luminex Corporation
12201 Technology Blvd., Suite 130
Austin, Texas 78727
USA
For information about disposal of FLEXMAP 3D outside of the European Union, contact
Luminex Technical Support at 1-877-785-2323 within the US and at +1-512-381-4397 outside
of the US.
For information about disposal of the barcode scanner, PC, or monitor, refer to the
manufacturer documentation.
Safety
9
FLEXMAP 3D® Hardware User Manual
10
This chapter reviews the technical aspects of theLuminex® FLEXMAP 3D® system: operation,
system components, subsystems, recommended additional equipment, and specifications.
How the FLEXMAP 3D System Operates
The Luminex® FLEXMAP 3D® system, in combination with xMAP® (Multi-Analyte Profiling)
technology, will simultaneously measure up to 500 analytes from a single sample. xMAP
technology incorporates microspheres, proprietary dyeing processes, flow cytometry based
fluidics, lasers, the latest in high-speed digital signal and advanced computer algorithms,
enabling a multi-analyte detection system that demonstrates excellent sensitivity and
specificity.
An established proprietary process created by Luminex to internally dye same-size
polystyrene microspheres with multiple fluorophores facilitates the creation of five hundred
distinguishable microspheres when these fluorophores are combined in varying
concentrations. The microspheres, discriminated by color, are excited by two lasers in the
FLEXMAP 3D instrument. The resulting emission is detected by avalanche photo diodes
(APDs) in three classification channels (CL1, CL2, and CL3) that is then further analyzed
using a separate APD in a doublet discriminator (DD) channel, which measures bead size
through side-scatter.
Analytes are bound to xMAP microspheres using the same surface chemistry used with
earlier Luminex instruments (LX100/200). Reporters, tagged with fluorescent labels excited at
a different wavelength than the internal dyes, bind to the analyte of interest and are detected
by a photomultiplier tube (PMT) in a reporter channel (RP1), allowing for quantitative
analysis. As the microspheres in a fluid stream pass rapidly through the laser beams, high-
speed digital signal and computer algorithms discriminate which analyte is being carried on
each microsphere and quantifies the reaction based on fluorescent reporter signal. The
results are analyzed by the system software and presented in a readable format for analysis.
Subsystems
The Luminex FLEXMAP 3D instrument includes four subsystems: electronic, fluidic,
mechanical, and optical. The followingtopics describe the user-accessible components of
each subsystem.
Chapter 2: Technical Overview
 
11
Electronics
The electronics system provides the power for operation and control of the FLEXMAP 3D
system and communication between its parts.
Power Input Module
The power input module contains the input power plug and fuses. This is the protective
earthing point for the FLEXMAP 3D system. The mating power cord connector type is
IEC-320-C13. The mating power cord provides electrical power to the instrument when it is
connected to an electrical outlet and is the means of disconnection. The power input auto-
senses the voltage range. See the "Back of the FLEXMAP 3D Instrument" image below.
WARNING: Do not obstruct this means of disconnection. Connect only to
outlets that contain protective earthing. Before changing a fuse,
turn off the instrument and unplug the power cord to avoid any
danger of electrical shock.
Communications Ports (USB Type B Connector)
The communications port connects the Luminex® FLEXMAP 3D® instrument to the computer.
See the "Back of the FLEXMAP 3D Instrument" image below.
FIGURE 8. Back of the FLEXMAP 3D Instrument
FLEXMAP 3D® Hardware User Manual
12
1 Power Input Module
2 Communications Port P1 (USB type B)
Fluidics
The fluidics system handles the flow of liquid through the Luminex® FLEXMAP 3D®
instrument.
Access Doors
The Luminex® FLEXMAP 3D® system has two front access doors. The left door supplies
access to a door ventilation filter and the sheath filter. The right door supplies access to a
door ventilation filter, the sample probe, and the syringe pumps. See the "Front of the
FLEXMAP 3D Instrument" image below.
FIGURE 9. Front of the FLEXMAP 3D Instrument
1 Left door, with access to sheath filter and door ventilation filter
2 Right door, with access to syringe pumps, door ventilation filter, and sample probe
Sample Probe
A stainless steel sample probe acquires the sample. See the "Sample Probe" image below.
WARNING: Avoid contact with moving parts.
Technical Overview
13
Cheminert® Fitting
This fitting attaches the sample probe to the sample tubing. Disconnect this fitting when you
remove the sample probe. See the "Sample Probe" figure below.
WARNING: Wear appropriate personal protective equipment when handling
parts that come into contact with potentially biohazardous
samples.
FIGURE 10. Sample Probe
1 Cheminert fitting
2 Attachment point on Cheminert fitting for sample tubing
3 Sample probe, in up position
Syringe Pump
The syringe delivers a sample from the microtiter plate to the cuvette. See the "Syringe
Pump" image below.
WARNING: Avoid contact with moving parts.
FLEXMAP 3D® Hardware User Manual
14
FIGURE 11. Syringe Pump
1 Syringe arm (within dotted lines)
2 Glass syringe barrel
3 Syringe plunger
Sheath Filter
The sheath filter removes particles greater than ten microns in diameter from the sheath fluid.
See the "Fluidics Bay" image below.
Fluidics Bay
The waste and sheath connectors, located on the front of the fluidics bay, connect to the
sheath fluid and waste fluid containers using clear tubing. The sheath fluid connector is blue
and the waste connector is orange. See the "Fluidics Bay" image below.
CAUTION: Wear appropriate personal protective equipment when handling
parts that come into contact with potentially biohazardous
samples.
Technical Overview
15
FIGURE 12. Fluidics Bay
1 Sheath filter
2 Tubing to sheath fluid container is attached here (blue)
3 Tubing to waste fluid container is attached here (orange)
Waste Fluid Container
The waste fluid container receives waste from the system. To maintain a stable flow rate, do
not move the waste line or container during system operation and make certain the waste
fluid container is properly vented. Place the container at least three feet below the surface on
which the instrument rests. You can place it as far away from the instrument as the tubing
allows. Never place it on top of the instrument. Refer to safety instructions for the waste fluid
container within the Fluidics section of the manual.
WARNING: Wear appropriate personal protective equipment when handling
parts that come into contact with potentially biohazardous
samples.
Mechanics
The mechanical subsystem of the FLEXMAP 3D instrument includes a filter system.
Replaceable ventilation filters clean the air used to cool the instrument and pressurize sheath
fluid. The four filters must be checked and cleaned as prescribed in the maintenance chapter
FLEXMAP 3D® Hardware User Manual
16
of this manual. The "Fluidics Bay" image above and "Front Door Ventilation Filters" image
below show the location of these filters.
FIGURE 13. Front Door Ventilation Filters
View A Right front door, viewed from inside
View B Left front door, viewed from inside
1 Filter
2 Holding clamp
FIGURE 14. XY and HEPA Filters
View A Front of XY, with XY cover removed
View B Front of fluidics bay, behind left front door
1 XY filter
Technical Overview
17
2 Holding clamp
3 Cover over HEPA air filter
Optics
The optical system consists of the optical assembly and the excitation lasers. The optical
components do not require manual adjustment by the user.
System Components
The following topics describe details of the three components of the Luminex® FLEXMAP 3D®
system: software, reagents, and hardware.
Luminex xPONENT® 4.0 Software
Luminex xPONENT 4.0 software provides complete control of the Luminex® FLEXMAP 3D®
instrument and performs the analyses. The software requires a dedicated PC. For updated
information about the PC or operating system, access http://www.luminexcorp.com. Click the
Support link to open the FAQ list.
Under most circumstances, the PC that comes with the FLEXMAP 3D system is preloaded
with xPONENT 4.0 software. Luminex provides a software CD to use if you need to reinstall
the software or need to install it on another computer. If you install the software on another
PC, be sure that the PC meets the minimum specifications, including 4.0 GB of RAM and a
2.66 GHz processor. The number of installations you can perform is limited by your license.
The software CD automatically installs the basic software only. To install the various
upgrades, contact Luminex Technical Support. A Technical Support representative can
supply you with the correct license number to install upgrades.
CAUTION: If you need to uninstall the software, follow carefully the
procedure provided by Luminex Technical Support.
The software is documented in two ways: online help, which can be accessed from within the
application itself, and in PDF form, which is available on the Luminex website and on CDs
included with the system.
CAUTION: Luminex recommends that you do not install additional software
on the PC that runs xPONENT 4.0. The operation of xPONENT
4.0 has been validated only when it is the only program running
on the dedicated PC.
Reagents
xMAP technology requires two kinds of reagents:
• Common laboratory reagents
• Reagents created especially for Luminex instruments
FLEXMAP 3D® Hardware User Manual
18
http://www.luminexcorp.com
CAUTION: Adhere to standard laboratory safety practices when handling
hazardous, toxic, or flammable reagents and chemicals. Contact
Luminex Technical Support when in doubt about compatibility of
cleaning and decontamination agents or materials.
Required Laboratory Reagents
• 10 - 20% household bleach
• 70% isopropanol or 70% ethanol
• 0.1N NaOH
• Mild detergent
• Distilled water
WARNING: Isopropanol and ethanol are flammable liquids. Keep them away
from heat, open flames, and sparks in a well-ventilated area.
Remove them from the instrument when they are not in use.
xMAP Technology Reagents
Luminex® FLEXMAP 3D® Hardware
The FLEXMAP 3D system includes the following hardware:
• The FLEXMAP 3D instrument
• Personal computer (PC) and accessories
• Power cables
• Three sample probes
• Off-plate reagent area
• 96 and 384 heater blocks
• Empty cubitainer for waste
• Sheath fluid intakeline
• Waste fluid outtake line
• USB communication cable
• Barcode reader (optional)
• Sample probe height alignment kit
• Monitor arm
NOTE: For information on calibrating the probe correctly, refer to the 
FLEXMAP 3D Probe Height Adjustment Tool section.
Recommended Additional Equipment
Successful operation of the Luminex FLEXMAP 3D system may require additional
equipment.
Technical Overview
19
Uninterruptible Power Supply (UPS) or Surge Protector
Luminex recommends using either an uninterruptible power supply (UPS) or a surge
protector to protect your system from power outages. Use a UPS that provides 1300 watts for
at least 45 minutes. Select a surge protector that fits your requirements with regard to
electrical environment, endurance, suppressed voltage rating, and method of protection. The
surge protector requires three outlets and a minimum rating of 1500 watts. Either piece of
equipment should be listed by Underwriters Laboratory (UL) or a similar listing body, certified
by Canadian Standards Association (CSA), and marked Conformité Europeénne (CE) for
nondomestic use.
Printer
Use a printer compatible with Microsoft® Windows XP SP3.
Barcode Labels
Use Code 128 barcode label type when scanning barcode labels into the system.
Vortex
Use VWR product number 58816-12, with a speed range of 0 to 3200 rpm, or equivalent.
Bath Sonicator
Use Cole-Parmer® product number 08849-00, with an operating frequency of 55 kHz, or
equivalent.
Specifications
The Luminex® FLEXMAP 3D® system is designed to fit a series of standards for speed,
accuracy, sensitivity, and capacity. The following topics provide the specifications for the
Luminex FLEXMAP 3D system.
CAUTION: Read and observe specifications carefully.
General
Indoor use only
Physical dimensions: 58.4 cm (23 inches) W x 65.3 cm (25.7 inches deep, including the required 1.25
inches for proper cooling) D x 54.7 cm (18.0 inches) H. Additional space required for the arm, monitor,
keyboard, mouse and optional barcode scanner does not exceed 64.8 cm (25.5 inches) W by 61 cm
(24.0 inches) D.
Weight: up to 91 kg (200 lbs), including the instrument, monitor arm, monitor and accessories such as
the keyboard, mouse, and barcode scanner.
Installation Category II
FLEXMAP 3D® Hardware User Manual
20
Pollution degree 2
Operating temperature: 15°C to 30°C (59°F to 86°F)
Operating humidity: 20% to 80%, noncondensing
Shipping and storage temperature: 0 to 50°C (32° F to 122° F)
Shipping and storage humidity: 20% to 80%, noncondensing
Temperature control: Maintains samples using the heater block at a constant temperature from 35°C
to 60°C (95°F to 131°F), +/- 1°C of setpoint.
Altitude: Operation up to 2400 m (7874 ft.) above mean sea level
Automatic transfer of assay protocols and new reagent information into the system using a large
capacity read/write DVD
Automatic sampling from a 96- or 384-well microtiter plate, beginning from any well position
Automatic real-time analysis
Analyzes multiple assay protocols per microtiter plate
Barcode reader entry of sample IDs
System initialization: < 45 minutes (including laser warmup, required software procedures, and weekly
calibration)
System verification: 5 minutes
FLEXMAP 3D warmup: 30 minutes. A system that remains inactive for at least four hours requires a
warm-up to restart the lasers. The system resets the four-hour internal clock after acquiring the
sample, running system calibrators, running system controls, or warming up the instrument.
Produces sound pressure levels below 85 dBA
Electronics
USB 2.0-compatible communications link for fast data transfer
Input voltage range: 100-120 V~, 6.0 A, 50/60 Hz or, 200-240 V~, 3.0 A, 50-60 Hz
Optics
Reporter channel detection: A/D resolution 16 bits
Reporter channel dynamic range: ≥ 4.5 decades of detection (verified with beads dyed with high levels
of organic dyes)
Reporter laser: 532 nm, nominal output 15.0+5%/-0% mW, diode-pumped; mode of operation,
continuous wave (CW); maximum output power 50 mW
Classification laser: 638 nm, nominal output 12.0 to 12.5 mW, diode; mode of operation, continuous
wave (CW); maximum output power 15 mW
Reporter detector: Photomultiplier tube, detection bandwidth of 565 to 585 nm
Technical Overview
21
Classification detector: Avalanche photo diodes with temperature compensation
Doublet discrimination detector: Avalanche photo diodes with temperature compensation
Fluidics
Cuvette: 200 micron square flow channel
Sample injection rate: 2 μL/second
Sample uptake volume: 20 to 200 μL
Sheath flow rate: 7.9 (+/- 0.9) mL/min
Sheath pressure: 8 to 13 psi for normal operations; 15 psi maximum
Microspheres
Distinguishes 1 to 500 unique xMAP microspheres in a single sample
Classification of xMAP microspheres: ≥ 80%
Total system misclassification of xMap microspheres: <= 4%
Internal sample carryover: < 1.5%
Detects a minimum of 500 fluorochromes of phycoerythrin (PE) per xMAP microsphere
Detects and distinguishes surface reporter fluorescence emissions at 575 nm on the surface of 1 to
500 unique xMAP microspheres in a single sample
Soluble background fluorescence emission at 575 nm automatically subtracted from fluorescence
intensity values.
Microtiter Plates
The Luminex® FLEXMAP 3D® system processes a 96-well microtiter plate in less than 20
minutes and a 384-well microtiter plate within 1 hour and 15 minutes, with 2500 microspheres
per region per well, counting 100 microspheres in each region.
Microtiter plates with 96 and 384 wells must be compatible with the microtiter heater block
temperature (from 35 to 60°C or 95° to 131°F) when the heater block is in use.
All microtiter plates (96 or 384 wells) have standard width (85.6 mm) and length (127.9 mm).
Depth varies depending on the type of well. To be compatible with the FLEXMAP 3D
instrument, the maximum allowable depth is 1”. Plates must have minimum 0.06” lip height,
standard distance from well center to well center (9 mm on 96-well plate), and standard
distance from A1 center to plate center in both length and width. To be compatible in size
with the microtiter heater block, the plate must fit into the heater block so that the top is flush
with the heater block.
FLEXMAP 3D® Hardware User Manual
22
To ensure accurate test results, properly clean and maintain the Luminex® FLEXMAP 3D®
system. Read and follow all instructions in this section. Perform adequate maintenance and
cleaning to avoid inaccurate results and potential hazards. To facilitate your maintenance
process, print out and use the maintenance logs found
General Maintenance Precautions
Observe the following general maintenance precautions, which were explained in more detail
in :
Personnel who use, maintain, or clean the FLEXMAP 3D instrument should be trained in
standard laboratory safety practices and should follow those practices when handling the
instrument.
Samples and waste fluid can contain biohazardous material. Where exposure to
biohazardous material, including in an aerosol form, exists, follow appropriate biosafety
procedures, use personal protective equipment, and use ventilation devices.
Do not remove the cover of the FLEXMAP 3D instrument under any circumstances.
Avoid contact with moving parts. Disconnect the instrument from the power source when
the procedure instructs you to do so.
Daily Maintenance
Chapter 3: Maintenance and Cleaning
 
23
Most of the daily maintenance tasks for the FLEXMAP 3D system, including system
initialization, warmup, and shutdown, can be performed using available software
commands. For details about the performance of these activities, see the appropriate
Luminex software manual or Luminex online help.
Initializing the FLEXMAP 3D Instrument
Use the software to perform system initialization.
The software provides three options for system initialization:
• Warmup, fluidics
• Warmup, fluidics, verification
• Warmup, fluidics, verification, and calibration
Select the option you want. Warmup, fluidics, and verificationshould be part of the daily
maintenance of the instrument; calibration can be limited to weekly maintenance unless
otherwise specified by assay instructions.
If the instrument temperature falls out of the delta cal range, recalibrate it. For
information about the delta cal range, see the appropriate Luminex software manual or
Luminex online help.
Warming Up the FLEXMAP 3D Instrument
If the FLEXMAP 3D instrument is powered on but idle for more than four hours, both
lasers turn off. Consequently, the instrument should be warmed up again. Use the
software to perform a warmup.
Maintaining Fluids
Periodically monitor fluid levels. Replace the empty sheath fluid container as needed. If the
Luminex® FLEXMAP 3D® instrument operates with an empty sheath fluid container, the lack
of sheath fluid may interrupt a sample and prevent further samples from being collected.
CAUTION: Use only xMAP Sheath Fluid or another Luminex-approved
sheath fluid. Use of any other sheath fluid constitutes improper
use and can void the warranty provided by Luminex and/or its
authorized partner.
Optimally, the waste line should be routed to a laboratory drain. If you use a waste container,
monitor waste fluid levels periodically. The volume of the waste container should be at least
as large as the volume of the sheath container. Do not allow the waste fluid container to
FLEXMAP 3D® Hardware User Manual
24
overflow. Empty the waste fluid container each time you replace or fill the sheath fluid
container.
Place the waste fluid container at least three feet below the surface on which the FLEXMAP
3D instrument rests. Do not place the waste fluid container on top of the instrument. Do not
move the waste line vertically while the FLEXMAP 3D instrument is running. Contact Luminex
Technical Support before relocating the waste fluid container or rerouting the waste line. You
can move the line temporarily for cleaning and maintenance.
To empty the waste fluid container:
1. Disconnect the waste fluid container from the FLEXMAP 3D instrument.
2. Discard the waste from the waste fluid container in accord with all local, state, federal,
and country specific biohazard handling regulations.
WARNING: Waste fluid can contain biohazardous infectious agents. Where
exposure to potentially biohazardous materials (including
aerosol) exists, follow appropriate biosafety procedures and use
personal protective equipment such as gloves, gowns,
laboratory coats, face shields (or mask and eye protection),
respirators, and ventilation devices.
Shutting Down the FLEXMAP 3D Instrument
Use the software to perform system shutdown. The shutdown procedure is intended to
shut down the FLEXMAP 3D instrument for short periods, for example, overnight, for a
weekend, or up to a week.
To shut down the FLEXMAP 3D instrument for a long period, see the section titled Storing
the FLEXMAP 3D Instrument.
Weekly Maintenance
Some of the weekly maintenance tasks for the FLEXMAP 3D system can be performed
using available software commands. For details about the performance of these
activities, see the Luminex xPONENT 4.0 software manual or Luminex online help.
Weekly maintenance includes using the software to perform a weekly maintenance
routine, removing clogs, cleaning the sample probe, calibrating the system, and visually
inspecting the instrument.
Running Weekly Maintenance
Use the software to perform a weekly maintenance routine.
Maintenance and Cleaning
25
Removing Clogs
If you frequently use the FLEXMAP 3D system to test concentrated serum or other
debris ridden samples, Luminex recommends that you use the software to perform a
clog removal routine weekly. Luminex recommends using sodium hydroxide (NaHO) to
perform an alcohol flush of the FLEXMAP 3D instrument to remove clogs.
To remove clogs:
1. Replace the alcohol in the alcohol well of the reservoir area with a 0.1N sodium hydroxide
solution.
2. Use the software to perform a clog removal routine. 
WARNING: Sodium hydroxide is extremely caustic. If it comes into contact
with skin, it can burn and cause tissue damage without causing
pain. Always wear gloves and goggles when working with
sodium hydroxide.
Cleaning the Sample Probe
WARNING: Avoid contact with moving parts. If a plate is running, use the
software to execute Stop to prohibit exposure to moving parts.
Refer to the software manual for instructions. The Luminex®
FLEXMAP 3D® system must not be performing any operations
while you carry out this maintenance procedure.
To clean the sample probe:
1. Use the software to execute STOP if a plate is running. Refer to the software manual for
instructions. The FLEXMAP 3D system must not be performing any operations while you
carry out this maintenance procedure.
2. Remove the sample probe.
a. Open the right front door of the FLEXMAP 3D instrument.
b. Unscrew the Cheminert fitting on top of the probe completely.
c. Grasp the probe gently and push up.
d. Lift the probe out of the top of the probe holder.
3. Clean the sample probe using either a bath sonicator or a 10 mL syringe. If you are using
a bath sonicator, place the tip of the sample probe in the bath sonicator for 2 to 5
minutes. If you are using a syringe, force distilled water through the tip of the sample
probe to its large end. This dislodges any debris clogging the tip.
FLEXMAP 3D® Hardware User Manual
26
4. Replace the sample probe and tightly screw in the Cheminert fitting.
5. Use the software to perform an automatic probe height adjustment. 
NOTE: Perform an automatic probe height adjustment any time the probe
is .
FIGURE 15. The Sample Probe
Calibrating the FLEXMAP 3D System
Calibrate the FLEXMAP 3D system weekly as part of regularly scheduled maintenance.
There are several different ways to calibrate the system using the software.
Visually Inspect the FLEXMAP 3D Instrument
Make sure the instrument is idle, so there are no moving parts. Open all of the FLEXMAP 3D
instrument doors and visually inspect for leaks, corrosion, and other signs of improper
function. Check all visible tubing connections.
Monthly Maintenance
Clean the exterior surfaces monthly.
To clean exterior surfaces:
Maintenance and Cleaning
27
1. Turn off the FLEXMAP 3D instrument and unplug the power cord.
2. Wipe all exterior surfaces with mild detergent, followed by a household bleach solution
diluted to 10% to 20%, followed by distilled water.
3. Open both doors of the instrument.
4. Clean all accessible surfaces with detergent, followed by the household bleach solution
(10% to 20%), followed by distilled water. 
WARNING: Avoid contact with the tubing and electronic parts of the
instrument.
5. Dry any unpainted metal surfaces to prevent corrosion.
6. Plug in the power cord and turn on the FLEXMAP 3D instrument.
Semi-annual Maintenance
Replacing the Syringe Teflon Seals
To replace each syringe seal:
1. Turn off the FLEXMAP 3D instrument and unplug the power cord. 
WARNING: A syringe arm does NOT deactivate while the seal is being
changed; unplugging is necessary to avoid injury.
2. Open the right front door of the FLEXMAP 3D instrument.
3. Locate the syringe (glass cylinder with a metal rod plunger).
4. Loosen the set screw on the syringe arm (at the bottom of the syringe) and push the
syringe arm down. 
NOTE: The syringe arm is tight. Be prepared to use some force to push it
down.
5. Unscrew the syringe from the top of its housing.
6. Pull the plunger out of the syringe.
7. Remove and replace the white plunger seal (at the top of the plunger) and the black O-
ring inside the seal.
8. Return the plunger to the syringe.
9. Screw the syringe back into its housing.
10. Return the syringe arm to its original position. The bottom of the plunger fits into the
indentation in the syringe arm.
11. Hand-tighten the set screw on the syringe arm.
12. Plug in the power cord and turn on the FLEXMAP 3D instrument.
FLEXMAP 3D® Hardware User Manual
28
13. Use the software to run the prime command twice, watching for any leaksin the syringe
area.
14. Close the right front door. 
FIGURE 16. Syringe Assembly (Inside Right Door)
Replacing the HEPA Air Filter
The HEPA air filter is the round filter behind the panel on the left side of the front of the
fluidics bay. Tubing from the interior of the fluidics bay is attached to the stem in the center
back of the filter.
To replace the HEPA air filter:
1. Turn off the FLEXMAP 3D instrument and unplug the power cord.
2. Open the left door and locate the HEPA air filter panel on the front of the fluidics bay as
shown in the HEPA Air Filter image below.
3. Remove the screw at the top of the panel and open the panel door as shown in the HEPA
Air Filter image below.
4. Grasp the tubing and pull the filter 3 to 4 inches from the unit.
Maintenance and Cleaning
29
5. Remove the filter with one hand and hold the tubing with the other hand. 
CAUTION: Do not allow the tubing to fall inside the instrument.
6. Connect a new filter to the tubing and position the filter inside the panel.
7. Reattach the panel door to the unit and close the access door.
8. Plug in the power cord and turn on the FLEXMAP 3D instrument. 
FIGURE 17. HEPA Air Filter
Cleaning the Ventilation Filters
Each filter has at least one imprinted arrow on its metal frame. This arrow indicates the
direction of the air flow. Filters should be installed so that the arrows are at the correct
locations and point in the correct direction.
To clean the Luminex® FLEXMAP 3D® ventilation filters:
1. Turn off the FLEXMAP 3D instrument and unplug the power cord.
2. Remove the XY cover by gently grasping its ventilation holes and pulling it straight out.
3. Slide clamps off the silver filter inside the cover and remove the filter. 
NOTE: For filter location, reference the "Ventilation Filter on XY Platform"
image below.
4. Open the front doors. Slide clamps off the left and right silver door filters and remove the
filters. 
NOTE: For clamp location, reference the "Ventilation Filters inside Doors"
image below.
5. Clean the filters with a vacuum or with distilled water.
FLEXMAP 3D® Hardware User Manual
30
6. Stand the filters upright to air dry. 
CAUTION: Filters must be completely dry prior to reinstallation.
7. Reinstall filters. The arrows on the door filters should point to the inside of the door. The
arrow on the XY filter should point up.
8. Reinstall the XY cover and close the access doors.
9. Plug in the power cord and turn on the FLEXMAP 3D instrument. 
FIGURE 18. Ventilation Filter on XY Platform
FIGURE 19. Ventilation Filters inside Doors
Maintenance and Cleaning
31
Annual maintenance requires replacing the sheath filter.
To replace the Luminex® FLEXMAP 3D® instrument sheath filter:
1. Turn off the FLEXMAP 3D instrument and unplug the power cord.
2. Open the left door on the FLEXMAP 3D instrument and locate the sheath filter on the
front of the fluidics bay.
3. Disconnect the filter by pushing down on the metal clamps at each quick-disconnect
point. 
NOTE: For information on the location of the disconnection clamps,
reference the "Front of Fluidics Bay (Inside Left Door)" image below.
4. Connect the new sheath filter, matching up the color-coded fittings. The arrow on the
sheath filter should be pointing up.
5. Close the left door.
6. Plug in the power cord and turn on the FLEXMAP 3D instrument.
7. Use the software to run the prime command twice. 
FIGURE 20. Front of Fluidics Bay (Inside Left Door)
FLEXMAP 3D® Hardware User Manual
32
Storing the FLEXMAP 3D Instrument
Storing the FLEXMAP 3D Instrument
This procedure explains the steps you should take before placing the FLEXMAP 3D
instrument into long-term storage.
To prepare the FLEXMAP 3D instrument for storage:
1. Use the software to perform a Preparation for storage routine.
2. Remove the sample probe from the instrument and flush it with distilled water from the
narrow end out through the larger end.
3. Replace the sample probe in the sample arm and wrap the end of the probe with
Parafilm®.
Preparing the FLEXMAP 3D Instrument for Use After
Storage
Follow this procedure before you begin using the FLEXMAP 3D instrument after its removal
from long-term storage.
To prepare the FLEXMAP 3D instrument for use after storage:
1. Make sure that the sheath container has a sufficient amount of sheath fluid and that the
waste fluid container is empty.
2. Remove the Parafilm from the end of the sample probe.
3. Turn on the FLEXMAP 3D instrument and watch for the following indications of correct
response: 
• The compressor starts. It has a low rumbling sound.
• Air blows out of the rear fans. Place your hand behind the FLEXMAP 3D instrument,
on the upper part of the back, to feel it.
NOTE: For the fan location, see the "Back of the FLEXMAP 3D Instrument"
image below.
• The syringes inside the right door of the FLEXMAP 3D instrument initialize.
4. Turn on the PC and start up the software. Use the software to run a revival after storage
routine.
Replacing Fuses
Periodically, you may need to replace a fuse on the FLEXMAP 3D instrument. The instrument
requires fuses with the following specifications:
F6A, 250 V
The fuse cartridge will accept either 5 mm x 20 mm or 0.25” x 1.25” fuses. Fuses are
available from Luminex Corporation. Replacing a fuse requires access to the back of the
FLEXMAP 3D instrument. Be aware before attempting any necessary lifting or movement of
the instrument that it weighs approximately 91 kg (200 lbs). Lifting it requires five people.
Maintenance and Cleaning
33
DANGER: To avoid serious injury or death by electric shock, you must turn
off the FLEXMAP 3D instrument and unplug it from the wall
before replacing a fuse.
To replace a fuse:
1. Unplug the power cable from the instrument.
2. Use a small, flat-blade screwdriver to open the module door on the lower left corner of
the back of the instrument. The door opens downward. Inside are two cartridges, a red
one on top and a black one on the bottom.
3. Use the screwdriver to remove the red cartridge.
4. Check both of the fuses in the cartridge for damage. A fuse can display physical evidence
of damage, for example, broken wire or blackened glass; if it displays no physical
evidence, test it for continuity with a voltmeter.
5. Replace damaged fuses with the type specified on the sticker to the right of the power
input module.
6. Replace the red cartridge.
7. Shut the module door.
8. Plug in the power cord and turn on the FLEXMAP 3D instrument. 
NOTE: If your FLEXMAP 3D instrument is not on a swivel base and it
requires moving in order for you to access the back of the
instrument, use the software to re-calibrate and re-verify the
instrument after it is restored to its original location.
FIGURE 21. Back of the FLEXMAP 3D Instrument
FLEXMAP 3D® Hardware User Manual
34
Maintenance Logs
Reproduce the following forms as necessary and use them to record maintenance
information. Fill in the dates in the first line of the table. The first table includes a sufficient
number of columns for one week (7 days). The second table includes a sufficient number of
columns for monthly maintenance (one task monthly), semi-annual maintenance (two tasks
twice yearly), and annual maintenance (two tasks yearly). For each item listed at the left,
enter your initials under each date on which you perform the task.
Short Term Maintenance - One Week
Date:
Daily
Maintenance
Initialize and
verify
Check fluid
levels
Shut down
Weekly Maintenance
Clean probe
Calibrate
Perform visual
inspection
Run weekly
maintenance
Run clog
removal
Long Term Maintenance - One Year
Date:
Monthly
Maintenance
Clean exterior
surfaces
Maintenance and Cleaning
35
Semi-annual
Maintenance
Clean ventilation
filters
Replace HEPA air
filter
Replace syringe
seals
Annual
Maintenance
Replace sheath
filter
FLEXMAP 3D® Hardware User Manual
36
Troubleshooting procedures help users identify and remedy problems with the instrument.
Overview
To troubleshoot a problem, locatethe symptom in one of the tables in this chapter, determine
the cause from the listed possibilities, and remedy it with the provided solution.
This chapter supplies information about the following topics:
• Power Supply Problems
• Communication Problems
• Clogs
• Pressurization Problems
• Fluid Leaks
• Sample Probe Problems
• Calibration Problems
• Verification Problems
• Acquisition Problems
• Bead Detail Irregularities
Contact Luminex Technical Support in the U.S. and Canada by calling 1-877-785-BEAD
(-2323). Outside of the U.S. and Canada, call +1 512-381-4397. Contact Luminex Technical
Support in Europe by calling +31 162 408 333. Email inquiries to support@luminexcorp.com.
Additional information is available on the Luminex website. Search the desired topic or
navigate through menus. Also, review the website’s FAQ section. Enter http://
www.luminexcorp.com in your browser’s address field. Click Support> Support Login to log
into the Support FAQ site.
This chapter does not troubleshoot problems with the PC. For help with PC problems, please
contact the technical support department for the manufacturer of your PC.
Chapter 4: Troubleshooting
 
37
mailto: support@luminexcorp.com
http://www.luminexcorp.com
http://www.luminexcorp.com
Power Supply Problems
Power supply problems often involve a blown fuse, faulty electronic component, or
disconnected cable.
CAUTION: Whenever you deal with a potential electrical problem, be careful
to avoid electrical shocks.
TABLE 1. Power Supply Problems
Symptom Possible cause Solution
The FLEXMAP 3D
instrument will not turn on.
The power cord is disconnected. Plug in the power cord.
No voltage is coming from the
electrical outlet.
Verify that the electrical outlet is
operational.
The power supply is faulty. Contact Technical Support.
A fuse has burned out See the section titled Replacing
Fuses. Use extreme care when
replacing a fuse.
Fuses continue to open
(blow).
A component has a short circuit. Contact Technical Support.
Communication Problems
Communication problems described in this section involve the links between the data system
(PC and software) and the Luminex® FLEXMAP 3D® instrument. This section does not
address communication issues with other peripheral devices.
“Communication” refers to the transfer of data between the PC and the FLEXMAP 3D
instrument, including the current status of the instrument, instrument control, sample
acquisition, session uploading, and start, stop, and pause features.
TABLE 2. Communication Problems
Symptom Possible cause Solution
The PC cannot establish
communication with the
FLEXMAP 3D instrument.
The communication cable is
unplugged or plugged into the
wrong port.
Plug in or move the
communications cable.
The FLEXMAP 3D instrument is
not turned on.
Turn off the PC. Turn on the
FLEXMAP 3D instrument and then
turn on the PC.
FLEXMAP 3D® Hardware User Manual
38
Clogs
Often, a clog somewhere in the Luminex® FLEXMAP 3D® instrument is the cause of a fluid
leak, a pressurization or sample probe problem, or a problem with calibration, verification, or
data acquisition.
To determine whether there is a clog, check the bead count during calibration. During
calibration you should see 300 bead events per second or more. If there are fewer than that,
there is probably a clog in the instrument.
In the event you encounter a problem that is clog-related, use the following procedure.
To troubleshoot a possible clog:
1. Clean and adjust the sample probe. See the section titled “Cleaning the Sample Probe”.
2. Perform the procedure for removing clogs. See the section titled “Removing Clogs”.
3. Replace all fluids.
4. Use the software to run the weekly maintenance routine.
5. Run calibration and verification.
If this procedure is unsuccessful, call Luminex Technical Support.
Pressurization Problems
Normal air and sheath pressure readings vary between 8 and 13 psi while the compressor
runs. If the instrument pressure is out of range, sample acquisition either can fail or can
return inadequate results.
TABLE 3. Pressurization Problems
Symptom Possible cause Solution
Pressurization fails or
pressure is too low.
The sheath and waste lines are
not fully connected.
Check the lines between the
sheath and waste bottles and the
Luminex FLEXMAP 3D
instrument.
The sheath or waste fittings are
cracked.
Inspect the fittings to be sure they
form a tight seal. If not, call
Technical Support.
There is a leak in the instrument. Check for leaks. A leak is obvious
if there is fluid on the surface
where the FLEXMAP 3D
instrument sits. See the section
titled “Fluid Leaks”.
The compressor does not engage. Use the software to run a prime
command. If you do not hear the
compressor turn on, contact
Technical Support.
Troubleshooting
39
Fluid Leaks
Fluid leaks can result in poor pressurization and failed sample acquisition.
TABLE 4. Fluid Leaks
Symptom Possible cause Solution
Fluid is pooled around the
FLEXMAP 3D instrument.
Fittings or fluid lines are damaged. Turn off and disconnect the
instrument to avoid electrical
shock and contact Technical
Support.
Fluid drips from the sample
probe.
The sample probe is clogged. See the section titled “Clogs”.
The sample valve is faulty. Contact Technical Support.
Fluid leaks from the front of
the instrument.
The syringe seal leaks. Replace the syringe seal. See the
section titled “Replacing the
Syringe Teflon Seals”.
The syringe valve leaks. Hand-tighten the syringe
connection (silver knob) on the
syringe valve. Use the software to
run a prime command. If leaks
continue, contact Technical
Support.
Sample Probe Problems
Problems with the sample probe can lead to fluid leaks and pressurization problems, and
they can inhibit sample acquisition.
TABLE 5. Sample Probe Problems
Symptom Possible cause Solution
The sample probe leaks. The sample probe is clogged. See the section titled “Clogs”.
The sample arm is stuck in
the up or down position.
The sample probe motor
connections are loose.
Contact Technical Support.
The sample probe motor is faulty. Contact Technical Support.
Probe calibration not done. Perform Automatic Probe Height
Adjustment using the software.
The sample arm does not go
down smoothly.
The microtiter plate is incorrectly
seated.
Adjust the microtiter plate.
FLEXMAP 3D® Hardware User Manual
40
Symptom Possible cause Solution
The microtiter plate is warped. Inspect the microtiter plate.
Replace it if it is warped.
The sample probe is bent. Remove the sample probe from
the instrument. Roll it on a flat
surface. If a sample probe has
been bent and rolled straight more
than once, discard it and replace it
with a new sample probe. Perform
an automatic sample probe height
adjustment using the software.
Calibration Problems
TABLE 6. Calibration Problems
Symptom Possible cause Solution
Calibration is slow or fails. The calibration microspheres are
not fully suspended.
Vortex the calibration vials to
resuspend the microspheres.
The wrong lot number or target
values are entered in the
appropriate software user
interface.
Check the lot number and target
values to make sure they are
correct.
The instrument calibrators are in
the wrong well.
Verify that calibration
microspheres are in the correct
well.
There are not enough calibrator
microspheres in the well.
Add at least five drops of calibrator
microspheres to the well. For
accurate drop volume, hold the
vial upside down at a 90 degree
angle to the microtiter plate while
dispensing them.
The calibrator lot is expired. Use an unexpired bottle of
calibrator microspheres.
The sample probe height is
incorrect.
Perform an automatic sample
probe height adjustment.
The sample probe is clogged. See the section titled "Clogs".
There is a partial clog in the
instrument.
See the section titled "Clogs".
Troubleshooting
41
Symptom Possible cause Solution
There is air in the instrument. The
waste line was moved during
instrument operation, resulting in
an unstable flow rate.
Performan automatic sample
probe height adjustment. Use the
software to run a prime command
three times, an alcohol flush
command twice, then a wash
command three time with distilled
water.
The waste line was moved during
instrument operation, resulting in
an unstable flow rate.
Stabilize the waste line during
instrument operation. See the
section titled “Maintaining Fluids”.
There is a problem internal to the
instrument.
Review the log of calibration
reports. Check for dramatic
changes in temperature, sheath
pressure, or voltage. If any of
these are present, contact
Technical Support.
No events are collected
during calibration.
There is a problem with fluid
levels.
Check the sheath and waste fluid
levels. Verify that tubing for both
bottles is tightly connected to the
instrument. Check that the waste
fluid container cap is vented.
The sample probe is clogged. See the section titled "Clogs".
The Cheminert fitting is loose. Tighten the Cheminert fitting.
Verification Problems
TABLE 7. Verification Problems
Symptom Possible cause Solution
The instrument fails
verification.
The verification microspheres are
not fully suspended.
Vortex the verification vials to
resuspend the microspheres.
The wrong lot number or target
values are entered in the
appropriate page of the software
UI.
Check the lot number and target
values to make sure they are
correct.
The instrument verification
microspheres are in the wrong
well.
Verify that verification
microspheres are in the correct
well.
There are not enough verification
microspheres in the well.
Add at least five drops of
verification microspheres to the
well. For accurate drop volume,
hold the vial upside down at a 90
degree angle to the microtiter plate
while dispensing them.
FLEXMAP 3D® Hardware User Manual
42
Symptom Possible cause Solution
The verification lot is expired. Use an unexpired bottle of
verification microspheres.
The verification microspheres
have been diluted.
Do not dilute the verification
microspheres.
The sample probe height is
incorrect.
Perform an automatic sample
probe height adjustment.
The sample probe is clogged. See the section titled “Clogs”.
There is air in the instrument. Verify the sample probe height.
Use the software to run a prime
command three times, an alcohol
flush command twice, then a wash
command three times with distilled
water.
The waste line was moved during
instrument operation, resulting in
an unstable flow rate.
Stabilize the waste line during
instrument operation. See the
section titled “Maintaining Fluids”.
There is a problem internal to the
instrument.
Review the log of calibration
reports. Check for dramatic
changes in temperature, sheath
pressure, or voltage. If any of
these are present, contact
Technical Support.
Acquisition Problems
TABLE 8. Verification Problems
Symptom Possible cause Solution
Acquisition fails or is slow. The air pressure is out of range. See the section titled
“Pressurization Problems”.
The sample probe height is
incorrect.
Perform an automatic sample
probe height adjustment.
The sample probe is clogged. See the section titled “Clogs”.
The sheath or waste lines are not
fully connected.
Disconnect and reconnect the
lines; you should hear them click.
The calibration microspheres have
expired.
Replace old microspheres with an
unexpired lot.
The wrong wells are selected for
the calibration microspheres.
Ensure that the correct wells are
selected in the software.
Troubleshooting
43
Symptom Possible cause Solution
The wrong lot number or target
values are entered in the
appropriate page of the software
UI.
Check the lot number and target
values to make sure they are
correct.
Air is present in the instrument. Verify the sample probe height.
Use the software to run a prime
command three times, an alcohol
flush command twice, then a wash
command three times with distilled
water.
The acquisition volume is set too
high.
Set the acquisition volume to at
least 25 μL less than the actual
volume in your wells. This enables
the probe to acquire sample more
efficiently with less chance of
incorporating air in the sample.
The xMAP microspheres are not
fully suspended.
Gently vortex the microtiter plate
or resuspend beads with a
multichannel pipettor to ensure
that the microspheres are present
in the solution.
The microspheres are
photobleached.
Replace the microspheres with
unphotobleached microspheres.
There are an insufficient number
of beads in the sample.
Ensure that there are 2000 to
5000 beads per bead set per well.
The sample is too concentrated. Dilute concentrated biological
fluids, such as serum or plasma, at
least 1:5.
Bead Detail Irregularities
Use these tools to assist in diagnosing system and kit-related problems:
• instrument calibrators
• instrument verifiers
• assay standards
• assay controls
• error messages
Review calibration/control trend reports routinely to detect trends.
Use instrument xMAP verification microspheres to check the success of the instrument
calibration and to troubleshoot. If there is a problem with kit results, xMAP verifiers can help
determine if the problem is related to the instrument. If calibration and verification are
successful, contact the kit manufacturer.
The Bead Detail Irregularities table displays irregular groupings of beads. Compare the
displays to the following bead detail of a normal bead grouping - a tight bead population
within a white region.
FLEXMAP 3D® Hardware User Manual
44
FIGURE 22. Normal Bead Detail
TABLE 9. Bead Detail Irregularities
Symptom Description Possible Problem Solution
xMAP and calibrator microspheres form a group in the wrong location.
Calibrator
microspheres
group together at
the top of the
region.
The calibrator
microspheres are
photobleached.
Replace the calibrator microspheres
with unphotobleached calibrator
microspheres. To avoid photobleaching,
protect microspheres from light.
xMAP
microspheres
group together at
the lower right of
the region.
The xMAP
microspheres are
photobleached.
Replace the xMAP microspheres with
unphotobleached xMAP microspheres.
To avoid photobleaching, protect
microspheres from light.
xMAP microspheres form an unusual shape.
xMAP
microspheres
form a long
diagonal line.
The xMAP
microspheres have
agglutinated.
Contact Technical Support.
xMAP
microspheres
form a group with
extending tails.
The solvent is
incompatible.
The Luminex Technical Support website
(http://www.luminexcorp.com/Support)
lists incompatible solvents. If the solvent
you are using is listed, switch solvents.
xMAP microspheres fail to form a group.
xMAP
microspheres do
not form a
cohestive group.
The sheath fluid is
incompatible.
Use only Luminex xMAP sheath fluid in
the FLEXMAP 3D instrument. Other
fluids can damage the instrument and
void your warranty.
Troubleshooting
45
http://www.luminexcorp.com/Support
Symptom Description Possible Problem Solution
xMAP
microspheres do
not form a
cohesive group
and are
somewhat
scattered.
There is air in the
instrument.
Verify the sample probe height. Use the
software to run a prime command three
times, an alcohol flush command twice,
then a wash command three times with
distilled water.
xMAP
microspheres are
widely scattered.
The sheath fluid is
empty.
Make sure there is sheath fluid in the
sheath container. Use the software to
run a prime command until all air is
pushed out of the instrument.
FLEXMAP 3D® Hardware User Manual
46
Shipping
If a serious problem arises with the FLEXMAP 3D system, it may be necessary to return it to
Luminex Corporation for repairs. If Luminex Technical Support directs you to return the
FLEXMAP 3D system, the Technical Support representative will provide you with all
necessary information as well as give you a Return Material Authorization (RMA) number.
NOTE: Before the instrument is returned, two procedures must be
performed: The instrument must be decontaminated and its reservoir
must be drained. For the decontamination procedure, see the section
titled “DecontaminationProcedure”. For instructions on draining the
reservoir, see the section titled “Draining the Reservoir”.
Draining the Reservoir
To drain the reservoir:
1. Make sure the waste fluid container can hold at least one additional liter of fluid.
2. Disconnect the sheath fluid tubing from the sheath fluid container.
3. Disconnect the sheath filter tubing (white connector) from its bottom quick disconnect
point (white connection) on the fluidics bay.
4. Disconnect the waste fluid tubing (orange connector) from the orange connection on the
fluidics bay.
5. Disconnect the sheath fluid tubing (blue connector) from the blue connection on the
fluidics bay.
Appendix A: Shipping
 
47
6. Connect the waste fluid tubing (orange connector) to the bottom quick disconnect point
(white connection) of the sheath filter tubing.
7. Use the software to run a warmup command. This starts the compressor, which drains
the sheath fluid from the reservoir into the waste fluid container. 
NOTE: To refill the reservoir, reconnect each connector to its color-matched
connection and use the software to run a prime command.
FIGURE 23. Front of Fluidics Bay
1 White connection for sheath fluid tubing - Disconnect as referenced in Step 3
2 Orange connection for waste fluid tubing - Disconnect as referenced in Step 4
3 Blue connection for sheath filter tubing - Disconnect as referenced in Step 5
Shipment Checklist
Complete the following checklist, sign and date it, and return it with the FLEXMAP 3D
system.
1. Remove all specimens from the instrument.
2. Decontaminate the instrument. See the section titled “Decontamination Procedure”.
3. Drain the reservoir. See the section titled “Draining the Reservoir”.
Was there an internal leak in the system?
FLEXMAP 3D® Hardware User Manual
48
Yes
No
Printed Name _____________________________________________________
Signature ________________________________________________________
Company/Institution ________________________________________________
Date________________ Instrument Serial No.___________________________
Shipping
49
FLEXMAP 3D® Hardware User Manual
50
Installation of the FLEXMAP 3D
The following installation instructions for FLEXMAP 3D are to be used for site prep,
assessing facilities requirement and in the event cabling or tubing must be reconnected.
To install FLEXMAP 3D:
Appendix B: Installation
 
51
1. Place the PC and monitor on the right side of the instrument.
2. Connect the FLEXMAP 3D instrument and all necessary peripheral devices to the PC. 
FIGURE 24. Back of PC
1 Monitor Plug 4 Barcode Scanner Plug
2 FLEXMAP 3D Communications Plug (USB
type A )
5 Monitor Plug (Touchscreen monitors only)
3 Mouse Plug 6 Power Inlet
FIGURE 25. Back of FlexMAP 3D Instrument
FLEXMAP 3D® Hardware User Manual
52
1 Power Inlet 2 PC Communications plug (USB type B)
3. Connect the USB communications cable between the FLEXMAP 3D instrument (P1) and
the PC, and connect the power cables for the PC, monitor, and FLEXMAP 3D instrument
to outlets but do not turn on any of the devices.
4. Place the sheath container below the instrument with the container opening facing up.
5. Remove the existing container cap and install the cap from the blue tubing accessory in
its place.
6. Connect the blue sheath line to both the disconnect on the sheath container and the blue
connector on the outside of the instrument. 
FIGURE 26. Sheath and Waste Container Connections
7. Place the empty waste container below the instrument with the container opening facing
up.
8. Remove the existing container cap and install the cap from the orange tubing accessory
in its place.
9. Connect the orange waste line to both the disconnect on the waste container and the
orange connector on the outside of the instrument.
10. Open the two front access doors.
11. Turn on the FLEXMAP 3D instrument using the power switch on the right side of the
instrument, toward the back.
12. Observe the indicators associated with powering up a FLEXMAP 3D instrument.
13. Visually inspects for leaks.
14. Turn on the PC and monitor.
15. Close the access doors.
16. If the system includes a touch screen monitor, test it by touching the screen. The mouse
cursor should move to the location you touched. If monitor does not respond, use the
supplied CD to install the drivers. If the mouse cursor responds correctly, calibrate the
monitor using the software preloaded onto the PC.
17. Access the Luminex xPONENT software on the PC and confirm that the instrument is
communicating with the software.
Installation
53
18. Calibrate the probe height using the instructions in the software user manual. 
NOTE: For information on calibrating the probe correctly, refer to the 
FLEXMAP 3D Probe Height Adjustment Tool section.
19. Calibrate the instrument using the instructions in the software user manual. 
FLEXMAP 3D® Hardware User Manual
54
Installation
55
FLEXMAP 3D® Hardware User Manual
56
Hardware
Product Description Customer Number
Luminex® FLEXMAP 3D® with xPONENT 4.0 FLEXMAP-3D
Heater Block for 96-well microtiter plates CN-0224-01
Heater Block for 384-well microtiter plates CN-0223-01
Bar Code Scanner CN-PC03-01
Air Filter: Access Door CN-0222-01
Air Filter: XY Platform CN-0229-01
Air Filter: HEPA CN-0228-01
Syringe Cylinder with seal CN-0013-01
Syringe Seal (Quantity 4) CN-0014-01
Sheath Filter with quick disconnect CN-0010-01
Sample Probe Height Adjustment Kit CN-0015-01
Cable, USB (A to B) CN-0227-01
Cable, Power CN-PXXX-01
Fuse (6 amp) CN-0226-01
Sample Probe Needle CN-0221-01
Off-plate Reservoir CN-0225-01
Automated Maintenance Plate CN-0206-01
PM (Preventive Maintenance) Kit CN-0215-01
Appendix C: Part Numbers
 
57
Software
Product Description Customer Number
Luminex xPONENT 4.0 Software for FLEXMAP 3D CN-SW16-01
Reagents
Product Description Customer Number
xMAP Sheath Fluid, LX100 40-50000
FLEXMAP 3D Calibration Kit F3D-CAL-K25
Microspheres, F3DCAL1 - Classification Calibrator F3DCAL1-05
Microspheres, F3DeCAL1 - Classification Calibrator F3DeCAL1-05
Microspheres, F3DCAL2 - Reporter Calibrator F3DCAL2-05
Microspheres, F3DCAL3 - EDR Calibrator F3DCAL31-05
FLEXCAP 3D Performance Verification Kit F3D-PVER-K25
Microspheres, F3DVER1 - Classification Verifier F3DVER1-05
Microspheres, F3DeVER1 - Classification Verifier F3DeVER2-05
Microspheres, F3DVER2 - Reporter Verifier F3DVER3-05
Microspheres, Fluidics1 - xMAP Fluidics 1 FLUID1-05
Microspheres, Fluidics2 - xMAP Fluidics 2 FLUID2-05
FLEXMAP 3D® Hardware User Manual
58
NOTE: The Probe Height Adjustment Tool is to be used for 384 well mylar
bottom and 384 well filter bottom plates only.
To calibrate the probe correctly, perform the following steps:
Appendix D: FLEXMAP 3D Probe Height
Adjustment Tool
 
59
1. Using data sheet for the plate you intend to calibrate the probe to, subtract the "well
depth" from the "plate height" as shown in the example below:
Plate Height: 14.2mm - Well Depth: 11.43mm = 2.77mm (2.8mm)
Example 1: 2.8mm or well C7 on the probe height adjustment tool for this example.
2. Click Eject from the System Status bar to eject the XY platform.
3. Place the probe height adjustment tool on the XY platform.
4. From the Home page click Probe and Heater.
5. On the Probe and Heater page, select the plate type (384 well) and enter a plate name.
6. Select the appropriate well to calibrate probe height (C7 for the example shown above).
7. Click Auto Adjust Height.
8. Click Save to save the plate settings.
Example 2: "C" Plate Height 14.4mm - "F" Well Depth 11.5mm = 2.9mm
FLEXMAP 3D® Hardware User Manual
60
2.9mm or well D7 on the probe height adjustment tool for this example.
FLEXMAP 3D Probe Height Adjustment Tool
61
	Table of Contents
	Safety
	Intended Use
	Warnings and Notes
	Symbols
	Testing and Certification
	Safety Precautions
	General
	Electromagnetic Compatibility
	Lasers
	Fluidics
	Biological Samples
	Mechanical Components
	Swivel Base
	Indicator Lights
	ElectricalComponents
	Heat
	Decontamination Procedure
	Installation of Instrument
	Disposal of Instrument
	Technical Overview
	How the FLEXMAP 3D System Operates
	Subsystems
	Electronics
	Power Input Module
	Communications Ports (USB Type B Connector)
	Fluidics
	Access Doors
	Sample Probe
	Cheminert® Fitting
	Syringe Pump
	Sheath Filter
	Fluidics Bay
	Waste Fluid Container
	Mechanics
	Optics
	System Components
	Luminex 	 xPONENT® 4.0 Software
	Reagents
	Required Laboratory Reagents
	xMAP Technology Reagents
	Luminex® 		 FLEXMAP 3D® Hardware
	Recommended Additional Equipment
	Uninterruptible Power Supply (UPS) or Surge Protector
	Printer
	Barcode Labels
	Vortex
	Bath Sonicator
	Specifications
	General
	Electronics
	Optics
	Fluidics
	Microspheres
	Microtiter Plates
	Maintenance and Cleaning
	General Maintenance Precautions
	Daily Maintenance
	Initializing the FLEXMAP 3D Instrument
	Warming Up the 	 FLEXMAP 3D Instrument
	Maintaining Fluids
	Shutting Down the FLEXMAP 3D Instrument
	Weekly Maintenance
	Running Weekly Maintenance
	Removing Clogs
	Cleaning the Sample Probe
	Calibrating the FLEXMAP 3D System
	Visually Inspect the FLEXMAP 3D Instrument
	Monthly Maintenance
	Semi-annual Maintenance
	Replacing the Syringe Teflon Seals
	Replacing the HEPA Air Filter
	Cleaning the Ventilation Filters
	
	Storing the FLEXMAP 3D Instrument
	Storing the FLEXMAP 3D Instrument
	Preparing the FLEXMAP 3D Instrument for Use After Storage
	Replacing Fuses
	Maintenance Logs
	Short Term Maintenance - One Week
	Long Term Maintenance - One Year
	Troubleshooting
	Overview
	Power Supply Problems
	Communication Problems
	Clogs
	Pressurization Problems
	Fluid Leaks
	Sample Probe Problems
	Calibration Problems
	Verification Problems
	Acquisition Problems
	Bead Detail Irregularities