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Benzene (Chapter 15) used to be a common laboratory solvent until OSHA (U.S. Occu-pational Safety and Health Administration) placed it on its list of carcinogens. Chemists now use methylbenzene (toluene) instead, which has very similar solvating power but is not carcino- genic. Why not? The reason is the relatively high reactivity of the benzylic hydrogens that renders methylbenzene subject to fast metabolic degrada- tion and extrusion from the body, unlike benzene, which can survive many days embedded in fatty and other tissues. Thus, the benzene ring, though itself quite unreactive because of its aromaticity, appears to activate neighboring bonds or, more generally, affects the chemistry of its substituents. You should not be too surprised by this fi nding, because it is complementary to the conclusions of Chapter 16. There we saw that substituents affect the behavior of benzene. Here we shall see the reverse. How does the benzene ring modify the behavior of neighboring reactive centers? This chapter takes a closer look at the effects exerted by the ring on the reactivity of alkyl substituents, as well as of attached hydroxy and amino functions. We shall see that the behavior of these groups (introduced in Chapters 3, 8, and 21) is altered by the occurrence of resonance. After considering the special reactivity of aryl-substituted (benzylic) carbon atoms, we turn our attention to the preparation and reactions of phenols and benzenamines (anilines). These compounds are found widely in nature and are used in synthetic procedures as precursors to substances such as aspirin, dyes, and vitamins. C H A P T E R T W E N T Y T W O Chemistry of Benzene Substituents Alkylbenzenes, Phenols, and Benzenamines Ciprofl oxacin (“Cipro”) is a synthetic tetrasubstituted benzene derivative used widely to treat bacterial infections, especially of the urinary tract. The photo shows a laboratory worker producing tablets of the drug. OO O N N N F 1020 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s 22-1 Reactivity at the Phenylmethyl (Benzyl) Carbon: Benzylic Resonance Stabilization Methylbenzene is readily metabolized because its methyl C – H bonds are relatively weak with respect to homolytic and heterolytic cleavage. When one of these methyl hydrogens has been removed, the resulting phenylmethyl (benzyl) group, C6H5CH2 (see margin), may be viewed as a benzene ring whose p system overlaps with an extra p orbital located on an attached alkyl carbon. This interaction, generally called benzylic resonance, stabilizes adjacent radical, cationic, and anionic centers in much the same way that overlap of a p bond and a third p orbital stabilizes 2-propenyl (allyl) intermediates (Section 14-1). However, unlike allylic systems, which may undergo transformations at either terminus and give product mixtures (in the case of unsymmetrical substrates), benzylic reactivity is regioselec- tive and occurs only at the benzylic carbon. The reason for this selectivity lies in the dis- ruption of aromaticity that goes with attack on the benzene ring. Benzylic radicals are reactive intermediates in the halogenation of alkylbenzenes We have seen that benzene will not react with chlorine or bromine unless a Lewis acid is added. The acid catalyzes halogenation of the ring (Section 15-9). Br–Br, FeBr3 –HBr Br Br–Br No reaction In contrast, heat or light allows attack by chlorine or bromine on methylbenzene (toluene) even in the absence of a catalyst. Analysis of the products shows that reaction takes place at the methyl group, not at the aromatic ring, and that excess halogen leads to multiple substitution. � CH2Br Br2� CH2H HBr� (Bromomethyl)- benzene CH3 (Chloromethyl)- benzene CH2Cl CHCl2 (Dichloromethyl)- benzene CCl3 (Trichloromethyl)- benzene Cl2, hv �HCl Cl2, hv �HCl Cl2, hv �HCl Each substitution yields one molecule of hydrogen halide as a by-product. As in the halogenation of alkanes (Sections 3-4 through 3-6) and the allylic halogena- tion of alkenes (Section 14-2), the mechanism of benzylic halogenation proceeds through radical intermediates. Heat or light induces dissociation of the halogen molecule into atoms. One of them abstracts a benzylic hydrogen, a reaction giving HX and a phenyl- methyl (benzyl) radical. This intermediate reacts with another molecule of halogen to give the product, a (halomethyl)benzene, and another halogen atom, which propagates the chain process. Phenylmethyl (benzyl) system 2-Propenyl (allyl) system Sole site of reactivity Sites of reactivity REACTION C h a p t e r 2 2 1021 Mechanism of Benzylic Halogenation Initiation: Propagation: X Phenylmethyl (benzyl) radical CH2 �HX j X� j j CH2X XOX CH2OH jOXðð š� X2ðš�Xš� � or h� What explains the ease of benzylic halogenation? The answer lies in the stabilization of the phenylmethyl (benzyl) radical by the phenomenon called benzylic resonance (Figure 22-1). As a consequence, the benzylic C – H bond is relatively weak (DH8 5 87 kcal mol21, 364 kJ mol21); its cleavage is relatively favorable and proceeds with a low activation energy. Inspection of the resonance structures in Figure 22-1 reveals why the halogen attacks only the benzylic position and not an aromatic carbon: Reaction at any but the benzylic carbon would destroy the aromatic character of the benzene ring. Remember: Single-headed (“fi shhook”) arrows denote the movement of single electrons. MECHANISM H H C CH2 or A B C CH2 δ δ δ δ CH2 CH2CH2 Figure 22-1 The benzene p system of the phenylmethyl (benzyl) radical enters into resonance with the adjacent radical center. The extent of delocalization may be depicted by (A) resonance structures, (B) dotted lines, or (C) orbitals. Benzylic cations delocalize the positive charge Reminiscent of the effects encountered in the corresponding allylic systems (Section 14-3), benzylic resonance can affect strongly the reactivity of benzylic halides and sulfonates in nucleophilic displacements. For example, the 4-methylbenzenesulfonate (tosylate) of 4-methoxyphenylmethanol (4-methoxybenzyl alcohol) reacts with solvent ethanol rapidly via an SN1 mechanism. This reaction is an example of solvolysis, specifi cally ethanolysis, which we described in Chapter 7. Exercise 22-1 For each of the following compounds, draw the structure and indicate where radical halogenation is most likely to occur upon heating in the presence of Br2. Then rank the compounds in approximate descending order of reactivity under bromination conditions. (a) Ethylbenzene; (b) 1,2-diphenylethane; (c) 1,3-diphenylpropane; (d) diphenylmethane; (e) (1-methylethyl)benzene. 2 2 - 1 B e n z y l i c R e s o n a n c e S t a b i l i z a t i o n 1022 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s (4-Methoxyphenyl)methyl 4-methylbenzenesulfonate 1-(Ethoxymethyl)-4-methoxybenzene (A primary benzylic tosylate) CH3O CH2OS CH3O CH2OCH2CH3 O O B B CH3 CH3 CH3CH2OH� HO3S� SN1 Ethanolysis The reason is the delocalization of the positive charge of the benzylic cation through the benzene ring, allowing for relatively facile dissociation of the starting sulfonate. CH3O CH2 L �L� CH3CH2OH CH2 � �� š� CH3O� O ð CH2 CH3O�ð � CH2 Benzylic cation Octet form CH3O�ð CH3O�ð CH2 product CH2 CH3O�� Mechanism of Benzylic Unimolecular Nucleophilic Substitution Several benzylic cations are stable enough to be isolable. For example, the X-ray struc- ture of the 2-phenyl-2-propyl cation (as its SbF6 2 salt) was obtained in 1997 and shows the phenyl – C bond (1.41 Å) to be intermediate in length between those of pure single (1.54 Å) and double bonds (1.33 Å), in addition to the expected planar framework and trigonal arrangement of all sp2 carbons (Figure 22-2), as expected for a delocalized benzylic system. REACTION MECHANISM ANIM ATION ANIMATED MECHANISM: Benzylicnucleophilic substitution H3C H3C 1.41 Å 1.48 Å 1.43 Å 1.37 Å 1.40 Å 114° 116° 122°123° + Figure 22-2 Structure of the 2-phenyl-2-propyl cation. Exercise 22-2 Which one of the two chlorides will solvolyze more rapidly: (1-chloroethyl)benzene, C6H5CHCl, or chloro(diphenyl)methane, (C6H5)2CHCl? Explain your answer. A CH3 The above SN1 reaction is facilitated by the presence of the para methoxy substituent, which allows for extra stabilization of the positive charge. In the absence of this substituent, SN2 processes may dominate. Thus, the parent phenylmethyl (benzyl) halides and sulfonates undergo preferential and unusually rapid SN2 displacements, even under solvolytic condi- tions, and particularly in the presence of good nucleophiles. As in allylic SN2 reactions (Section 14-3), two factors contribute to this acceleration. One is that the benzylic carbon is made relatively more electrophilic by the neighboring sp2-hybridized phenyl carbon C h a p t e r 2 2 1023 (as opposed to sp3-hybridized ones; Section 13-2). The second is stabilization of the SN2 transition state by overlap with the benzene p system (Figure 22-3). CH2Br �CN� Br�� 81% Phenylethanenitrile (Phenylacetonitrile) (Bromomethyl)benzene (Benzyl bromide) CH2CN SN2 ( 100 times faster than SN2 reactions of primary bromoalkanes) ‡ C Nu −� X −� H H Figure 22-3 The benzene p system overlaps with the orbitals of the SN2 transition state at a benzylic center. As a result, the transition state is stabilized, thereby lowering the activation barrier toward SN2 reactions of (halomethyl)benzenes. ANIM ATION ANIMATED MECHANISM: Benzylic nucleophilic substitution Exercise 22-3 Phenylmethanol (benzyl alcohol) is converted into (chloromethyl)benzene in the presence of hydrogen chloride much more rapidly than ethanol is converted into chloroethane. Explain. Resonance in benzylic anions makes benzylic hydrogens relatively acidic A negative charge adjacent to a benzene ring, as in the phenylmethyl (benzyl) anion, is stabilized by conjugation in much the same way that the corresponding radical and cation are stabilized. The electrostatic potential maps of the three species (rendered in the margin at an attenuated scale for optimum contrast) show the delocalized positive (blue) and neg- ative (red) charges in the cation and anion, respectively, in addition to the delocalized electron (yellow) in the neutral radical. CH2� � � CH2 Resonance in Benzylic Anions � H� CH2 CH2ðCH3 k � k � 41pKa The acidity of methylbenzene (toluene; pKa < 41) is therefore considerably greater than that of ethane (pKa < 50) and comparable to that of propene (pKa < 40), which is de- protonated to produce the resonance-stabilized 2-propenyl (allyl) anion (Section 14-4). Consequently, methylbenzene (toluene) can be deprotonated by butyllithium to generate phenylmethyllithium. Phenylmethyl (benzyl) cation Phenylmethyl (benzyl) anion Phenylmethyl (benzyl) radical 2 2 - 1 B e n z y l i c R e s o n a n c e S t a b i l i z a t i o n 1024 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s Deprotonation of Methylbenzene � CH3CH2CH2CH2Li CH3 Methylbenzene (Toluene) � CH3CH2CH2CH2H CH2Li Phenylmethyllithium (Benzyllithium) (CH3)2NCH2CH2N(CH3)2, THF, � Exercise 22-4 Which molecule in each of the following pairs is more reactive with the indicated reagents, and why? (a) (C6H5)2CH2 or C6H5CH3, with CH3CH2CH2CH2Li (b) OCH3 or , with NaOCH3 in CH3OH CH2Br OCH3 CH2Cl (c) or , with HCl CH3CHOH NO2 CH3CHOH In Summary Benzylic radicals, cations, and anions are stabilized by resonance with the benzene ring. This effect allows for relatively easy radical halogenations, SN1 and SN2 reactions, and benzylic anion formation. 22-2 Benzylic Oxidations and Reductions Because it is aromatic, the benzene ring is quite unreactive. While it does undergo electrophilic aromatic substitutions (Chapters 15 and 16), reactions that dismantle the aromatic six-electron circuit, such as oxidations and reductions, are much more diffi cult to achieve. In contrast, such transformations occur with comparative ease when taking place at benzylic positions. This sec- tion describes how certain reagents oxidize and reduce alkyl substituents on the benzene ring. Oxidation of alkyl-substituted benzenes leads to aromatic ketones and acids Reagents such as hot KMnO4 and Na2Cr2O7 may oxidize alkylbenzenes all the way to benzoic acids. Benzylic carbon – carbon bonds are cleaved in this process, which usually requires at least one benzylic C – H bond to be present in the starting material (i.e., tertiary alkylbenzenes are inert). CH2CH2CH2CH3 1-Butyl-4-methylbenzene H3C COOH 1,4-Benzenedicarboxylic acid (Terephthalic acid) 80% Complete Benzylic Oxidations of Alkyl Chains HOOC 1. KMnO4, HO�, � 2. H�, H2O �3 CO2 C h a p t e r 2 2 1025 The reaction proceeds through fi rst the benzylic alcohol and then the ketone, at which stage it can be stopped under milder conditions (see margin and Section 16-5). The special reactivity of the benzylic position is also seen in the mild conditions required for the oxidation of benzylic alcohols to the corresponding carbonyl compounds. For example, manganese dioxide, MnO2, performs this oxidation selectively in the presence of other (nonbenzylic) hydroxy groups. (Recall that MnO2 was used in the conversion of allylic alcohols into a,b-unsaturated aldehydes and ketones; see Section 17-4.) OH OH OHH CH3O CH3O CH3O Selective Oxidation of a Benzylic Alcohol with Manganese Dioxide CH3O MnO2, acetone, 25°C, 5 h 94% O Benzylic ethers are cleaved by hydrogenolysis Exposure of benzylic alcohols or ethers to hydrogen in the presence of metal catalysts results in rupture of the reactive benzylic carbon – oxygen bond. This transformation is an example of hydrogenolysis, cleavage of a s bond by catalytically activated hydrogen. 1,2,3,4-Tetra- hydronaphthalene (Tetralin) Not isolated OH 71% 1-Oxo-1,2,3,4-tetrahydro- naphthalene (1-Tetralone) O CrO3, CH3COOH H2O, 21°C , Cleavage of Benzylic Ethers by Hydrogenolysis CH2 ORO H2, Pd–C, 25°C CH2H � HOR Hydrogenolysis is not possible for ordinary alcohols and ethers. Therefore, the phenyl- methyl (benzyl) substituent is a valuable protecting group for hydroxy functions. The following scheme shows its use in part of a synthesis of a compound in the eudesmane class of essential oils, which includes substances of importance in both medicine and perfumery. Exercise 22-5 Write synthetic schemes that would connect the following starting materials with their products. (a) CH3CH2CH3 (b) CH3H3C CH3H3C OO OO OO 2 2 - 2 B e n z y l i c O x i d a t i o n s a n d R e d u c t i o n s 1026 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s Because the hydrogenolysis of the phenylmethyl (benzyl) ether in the fi nal step occurs under neutral conditions, the tertiary alcohol function survives untouched. A tertiary butyl ether would have been a worse choice as a protecting group, because cleavage of its carbon – oxygen bond would have required acid (Section 9-8), which may cause dehydration (Section 9-2). In Summary Benzylic oxidations of alkyl groups take place in the presence of permanga- nate or chromate; benzylic alcohols are converted into the corresponding ketones by man- ganese dioxide. The benzylic ether function can be cleaved by hydrogenolysis in a transformation that allows the phenylmethyl (benzyl) substituent to be used as a protecting group for the hydroxy function in alcohols. 22-3 Names and Properties of Phenols Arenes substituted by hydroxy groups are called phenols (Section 15-1). The p system of the benzene ring overlaps with an occupied p orbital on the oxygen atom, a situation result- ing in delocalization similar to that found in benzylic anions (Section 22-1). As one result of this extended conjugation,phenols possess an unusual, enolic structure. Recall that enols are usually unstable: They tautomerize easily to the corresponding ketones because of the relatively strong carbonyl bond (Section 18-2). Phenols, however, prefer the enol to the keto form because the aromatic character of the benzene ring is preserved. O O 1. CH3Li, (CH3CH2)2O 2. H�, H2O (Section 8-8) H CH3% ≥ RO % CCH3 98% % A A CH3 OH H2, Pd–C, CH3CH2OH Deprotection of OR H CH3% ≥ HO % CCH3 98% % A A CH3 OH H CH3% ≥ RO % (Mixture of E and Z isomers) O 93% CH3CHP DMSO Wittig reaction (Section 17-12) P(C6H5)3, H CH3% ≥ RO % CHCH3 94% 1. BH3, THF 2. Oxidation (to alcohol) 3. Oxidation (to ketone) Hydroboration–oxidation* (Section 12-8) Oxidation (Section 8-6) H CH3% ≥ RO % COCH3 99% % O O O H CH3% ≥ 1. NaH, THF 2. C6H5CH2Br O O HO % H CH3% ≥ O O RO % Protection of OH (Section 9-6) CH3COOH, H2O Deprotection of carbonyl group (Section 17-8) 80% (R � C6H5CH2) Phenylmethyl Protection in a Complex Synthesis *While the literature reports the use of special oxidizing agents, in principle 2. H2O2, 2OH and 3. CrO3 would have been satisfactory. C h a p t e r 2 2 1027 Keto and Enol Forms of Acetone and Phenol 2,4-Cyclohexadienone O H �š H OHð šOHðð ð šOH �ð �šOH �š ð� �šOH Phenol K ~ 1013 2-PropenolAcetone K ~ 10�9 Oðð H C H2 H3C CH2H3C šOHð Phenols and their ethers are ubiquitous in nature; some derivatives have medicinal and herbicidal applications, whereas others are important industrial materials. This section fi rst explains the names of these compounds. It then describes an important difference between phenols and alkanols — phenols are stronger acids because of the neighboring aromatic ring. Phenols are hydroxyarenes Phenol itself was formerly known as carbolic acid. It forms colorless needles (m.p. 418C), has a characteristic odor, and is somewhat soluble in water. Aqueous solutions of it (or its methyl-substituted derivatives) are applied as disinfectants, but its main use is for the prep- aration of polymers (phenolic resins; Section 22-6). Pure phenol causes severe skin burns and is toxic; deaths have been reported from the ingestion of as little as 1 g. Fatal poison- ing may also result from absorption through the skin. Substituted phenols are named as phenols, benzenediols, or benzenetriols, according to the system described in Section 15-1, although some common names are accepted by IUPAC (Section 22-8). These substances fi nd uses in the photography, dyeing, and tanning indus- tries. The compound bisphenol A (shown in the margin; see also Chemical Highlight 22-1) is an important monomer in the synthesis of epoxyresins and polycarbonates, materials that are widely employed in the manufacture of durable plastic materials, food packaging, dental sealants, and coatings inside beverage cans (Chemical Highlight 22-1). Phenols containing the higher-ranking carboxylic acid functionality are called hydroxy- benzoic acids. Many have common names. Phenyl ethers are named as alkoxybenzenes. As a substituent, C6H5O is called phenoxy. OH NO2 Cl 4-Chloro- 3-nitrophenol COOH OH 3-Hydroxybenz- oic acid (m-Hydroxybenzoic acid) OH 1,4-Benzenediol (Hydroquinone) OH 1,2,3-Benzenetriol (Pyrogallol) OH OH 4-Methylphenol (p-Cresol) CH3 OH OH Many examples of phenol derivatives, particularly those exhibiting physiological activity, are depicted in this book (e.g., see Chemical Highlights 5-4, 9-1, 21-1, 22-1, and 22-2, and OH OH Bisphenol A CH3C CH3O O 2 2 - 3 N a m e s a n d P r o p e r t i e s o f P h e n o l s 1028 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s Phenols are unusually acidic Phenols have pKa values that range from 8 to 10. Even though they are less acidic than carboxylic acids (pKa 5 3 – 5), they are stronger than alkanols (pKa 5 16 – 18). The reason is resonance: The negative charge in the conjugate base, called the phenoxide ion, is stabilized by delocalization into the ring. OH H� � � šð Ošð ð Oð ð � � O Phenoxide ion Acidity of Phenol ð ðOð ð k � k � pKa 10 HO O H3C N O H Capsaicin (Active ingredient in hot pepper, as in jalapeño or cayenne pepper) A E OH OHHO Resveratrol (Cancer chemopreventive from grapes; see also Chemical Highlight 22-1) O O O OH OH OH OH OH OH HO HO ~ HO O 4-(4-Hydroxyphenyl)-2-butanone (Flavor of raspberries) Raspberries. Green tea. Chili peppers. Sections 4-7, 9-11, 15-1, 22-9, 24-12, 25-8, and 26-1). You are very likely to have ingested without knowing it the four phenol derivatives shown here. Epigallocatechin-3 gallate (Cancer chemopreventitive from green tea) C h a p t e r 2 2 1029 The acidity of phenols is greatly affected by substituents that are capable of resonance. 4-Nitrophenol (p-nitrophenol), for example, has a pKa of 7.15. OHšð H� etc. � � Ošð ð Oð ð � k � � N� �O Oð� HK �½ k N� �O Oð� HK �½ k N� �O Oð� HK �½ k �O H �½ k pKa � 7.15 Oð ð N� Oð� �O H �½ kO�½ Oð ð N� �ý E Negative charge delocalized into the nitro group The 2-isomer has similar acidity (pKa 5 7.22), whereas nitrosubstitution at C3 results in a pKa of 8.39. Multiple nitration increases the acidity to that of carboxylic or even mineral acids. Electron-donating substituents have the opposite effect, raising the pKa. O2NNO2 NO2 2,4-Dinitrophenol OH pKa � 4.09 NO2 NO2 2,4,6-Trinitrophenol (Picric acid) OH pKa � 0.25 CH3 4-Methylphenol ( p-Cresol) OH pKa � 10.26 As Section 22-5 will show, the oxygen in phenol and its ethers is also weakly basic, in the case of ethers giving rise to acid-catalyzed cleavage. Exercise 22-6 Why is 3-nitrophenol (m-nitrophenol) less acidic than its 2- and 4-isomers but more acidic than phenol itself? In Summary Phenols exist in the enol form because of aromatic stabilization. They are named according to the rules for naming aromatic compounds explained in Section 15-1. Those derivatives bearing carboxy groups on the ring are called hydroxy- benzoic acids. Phenols are acidic because the corresponding anions are resonance stabilized. Exercise 22-7 Rank in order of increasing acidity: phenol, A; 3,4-dimethylphenol, B; 3-hydroxybenzoic (m-hydroxybenzoic) acid, C; 4-(fl uoromethyl)phenol [p-(fl uoromethyl)phenol], D. 2 2 - 3 N a m e s a n d P r o p e r t i e s o f P h e n o l s 1030 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s CHEMICAL HIGHLIGHT 22-1 Two Phenols in the News: Bisphenol A and Resveratrol This section mentions two phenols as examples of chemicals to which you have potentially frequent exposure: bisphenol A (p. 1027) and resveratrol (p. 1028). Bisphenol A is the essential ingredient of the familiar polycarbonate plastics used in clear baby bottles, instant-formula cans, dental sealants, the linings of food and beverage containers, and reusable plastic bottles. More than 2 billion pounds are made each year in the United States alone, and three times as much are made worldwide. Yet there is continuing controversy surrounding this monomer, fi rst made in 1891— a controversy that illustrates the diffi culty of interpreting scientifi c data in terms of human risk assessment. The problem is that bisphenol A behaves as an estrogen mimic in animals. The monomer has been found to leach from the plastic, at increasing rates when heated, such as in a microwave oven. For example, a study carried out in 2003 showed that even very low levels of bisphenol A (about 20 ppb) caused chromosome aberrations in developing mouse eggs. These amounts are in the same range as are encountered in human blood and urine. Because the pro- cesses through which human and mouse eggs are prepared for fertilization are very similar, the study is a cause for concern, although it does not prove that humans are at risk. Moreover, in other studies carried out with adult rats,bisphenol A appeared to have no adverse effects on repro- duction and development. A critique of these fi ndings, dated 2008, pointed out that fetuses and newborns lack the liver enzyme needed to detoxify the chemical, again raising the specter of adverse effects on children. As a report by the National Institute of Environmental Health Sciences stresses, the discrepancies in studies of this type may be due to the use of different strains of animals, varying degrees of exposure and differing background levels of estrogenic pollutants, dosing regimens, and housing of the animals A A B O n O CC CH3 CH3 O O Carbonate unit O O Bisphenol A unit Polycarbonate plastics A polycarbonate plastic bottle in action. 22-4 Preparation of Phenols: Nucleophilic Aromatic Substitution Phenols are synthesized quite differently from the way in which ordinary substituted ben- zenes are made. Direct electrophilic addition of OH to arenes is diffi cult because of the scarcity of reagents that generate an electrophilic hydroxy group, such as HO1. Instead, phenols are prepared by formal nucleophilic displacement of a leaving group from the arene ring by hydroxide, HO2, reminiscent of, but mechanistically quite different from, the synthesis of alkanols from haloalkanes. This section considers the ways in which this transformation may be achieved. Nucleophilic aromatic substitution may follow an addition – elimination pathway Treatment of 1-chloro-2,4-dinitrobenzene with hydroxide replaces the halogen with the nucleophile, furnishing the corresponding substituted phenol. Other nucleophiles, such C h a p t e r 2 2 1031 (singly versus group). Remember that we are talking about extremely small concentrations of a biologically active com- pound (parts per billion!) that affect only a certain percent- age of animals and to differing degrees. And then there are the big questions of the extent to which animal studies are relevant to humans and whether there is a threshold level of exposure that humans can tolerate because of the presence of evolutionary natural detoxifi cation mechanisms. In 2007 and 2008, two government-convened groups reiterated that human exposure is at a level that causes adverse effects in humans and that there is concern about prenatal and early- childhood exposure. As a result, both Canada and the U.S. Congress began moving toward a ban of bisphenol A in children’s products, and some retailers, such as Wal-Mart and Toys“R”Us, are phasing out their sale. Concurrently, industrial producers are replacing polycarbonate plastics with other polymers. The issues surrounding the potentially harmful effects of chemicals in the environment are equally relevant with respect to their potentially benefi cial effects. An example is resveratrol. This compound has been used in traditional medicine to treat conditions of the heart and liver, and recently scientists took an interest in its physiological prop- erties. It is present in various plants and foods, such as eucalyptus, lily, mulberries, peanuts, and, most prominently, in the skin of white and, especially, red grapes, where it is found in concentrations of 50–100 mg/g. The compound is a chemical weapon against invading organisms, such as fungi. Grapes are used for wine making, so resveratrol occurs in red wine, at levels of up to 160 mg per ounce. Studies have suggested that the regular consumption of red wine reduces the incidence of coronary heart disease, a fi nding described as the “French paradox,” namely, the low incidence of heart problems in France despite the relatively high-fat diet. Resveratrol may be the active species, as recent research has indicated its potentially benefi cial cardiovascular effects, including its action as an antioxidant, which inhibits lipid peroxidation (Section 22-9), and as an antiplatelet agent (Chemical Highlight 22-2), which prevents atherosclerosis. Other investigations have shown that the molecule is also an active antitumor agent, involved in retarding the initia- tion, promotion, and progression of certain cancers, with seemingly minimal toxicity. Perhaps most interesting, it was discovered that resveratrol signifi cantly extended the lifespan of certain species of yeast, worm, the fruit fl y, and fi sh. Along similar lines, it canceled the life-shortening effects of a high-fat diet in mice. As a result of these promising discoveries, resveratrol has been hailed by manufacturers as the “French paradox in a bottle” and pushed for wide-scale consumption. However, experts advise caution. For example, little is known about its metabolism and how it affects the liver, the above results are derived in large part from in vitro experiments, and, like bisphenol A, it has estrogen-like physiological effects, enhancing the growth of breast cancer cells. For the time being, an occasional glass of red wine may be the best course of action, if any! Resveratrol protects grapes from fungus, such as that shown here. as alkoxides or ammonia, may be similarly employed, forming alkoxyarenes and arenamines, respectively. Processes such as these, in which a group other than hydro- gen is displaced from an aromatic ring, are called ipso substitutions (ipso, Latin, on itself). The products of these reactions are intermediates in the manufacture of useful dyes. NO2 � Nu� NO2 Cl ð NO2 � Cl� NO2 Nu Nucleophilic Aromatic Ipso Substitution Electron withdrawing Ipso position REACTION 2 2 - 4 N u c l e o p h i l i c A r o m a t i c S u b s t i t u t i o n 1032 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s The transformation is called nucleophilic aromatic substitution. The key to its suc- cess is the presence of one or more strongly electron-withdrawing groups on the benzene ring located ortho or para to the leaving group. Such substituents stabilize an intermedi- ate anion by resonance. In contrast with the SN2 reaction of haloalkanes, substitution in these reactions takes place by a two-step mechanism, an addition – elimination sequence similar to the mechanism of substitution of carboxylic acid derivatives (Sections 19-7 and 20-2). Mechanism of Nucleophilic Aromatic Substitution Step 1. Addition (facilitated by resonance stabilization) �N Cl � � NO2 EO � Nu �ð � Oð�š � Oð�š ðO� � ðð ð Oðð š� O �ðš� O �ðš� EO �ðš� EO �ðš� B k � k NO2 NO2 �N O NO2 Nu Cl NO2 ðð �š NO2 �N ONu Cl Nu Cl Nu ClNu Cl The negative charge is strongly stabilized by resonance involving the ortho- and para-NO2 groups. N�NE HN � E HN � Oð� NO2 NO2 Cl NO2 � NH4Cl NO2 85% 2,4-Dinitrobenzenamine (2,4-Dinitroaniline) NH2 NH3, �ð NO2 Na2CO3, HOH, 100°C NO2 90% 1-Chloro-2,4- dinitrobenzene 2,4-Dinitrophenol Cl NO2 � NaCl NO2 OH MECHANISM ANIM ATION ANIMATED MECHANISM Nucleophilic aromatic substitution C h a p t e r 2 2 1033 Step 2. Elimination (only one resonance structure is shown) N� NO2 EO �ð Oðð š� B N� EO � Cl � �ð Oðð š� ððš� Clðš� BNu �k NO2 Nu In the fi rst and rate-determining step, ipso attack by the nucleophile produces an anion with a highly delocalized charge, for which several resonance structures may be written, as shown. Note the ability of the negative charge to be delocalized into the electron-withdrawing groups. In contrast, such delocalization is not possible in 1-chloro-3,5-dinitrobenzene, in which these groups are located meta; so this compound does not undergo ipso substitution under the conditions employed. NO2 Cl O2N NO2O2N � k NO2O2NNO2O2N Meta-NO2 groups do not provide resonance stabilization of the negative charge. � � �k Nu �ð Nu Cl Nu Cl Nu Cl In the second step, the leaving group is expelled to regenerate the aromatic ring. The reactivity of haloarenes in nucleophilic substitutions increases with the nucleophilicity of the reagent and the number of electron-withdrawing groups on the ring, particularly ifthey are in the ortho and para positions. Exercise 22-8 Write the expected product of reaction of 1-chloro-2,4-dinitrobenzene with NaOCH3 in boiling CH3OH. 2 2 - 4 N u c l e o p h i l i c A r o m a t i c S u b s t i t u t i o n Exercise 22-9 Working with the Concepts: Using Nucleophilic Aromatic Substitution in Synthesis Ofl oxacin, an antibiotic of the quinolone class (Section 25-7), is used in the treatment of infections of the respiratory and urinary tract, the eyes, the ears, and skin tissue. The quinolone antibiotics are yet another alternative in the ongoing battle against penicillin (and other drug)-resistant bacteria. The fi nal three steps in the synthesis of ofl oxacin are shown below, the last of which is a simple ester hydrolysis (Section 20-4). Formulate mechanisms for the other two transformations from A to B and then to C. CH3 F F F OH F HN H3C O O O F O F O O Na�H�, 70–90°C O N CH3 CH3 F COH N O NaOH, H2O O N N N N H CH3 A , toluene, 100�C E B O CH3 CH3 F O N O O O N NE A OfloxacinC B Strategy Taking an inventory of the topological changes and of the nature of the bonds formed and bonds broken, we note that B and C are the result of two successive intra- and one intermolecular nucleophilic aromatic substitutions, respectively. The fi rst steps are ring closures and therefore espe- cially favored for entropic and enthalpic reasons. (Formation of six-membered rings is a favorable reaction, see Sections 9-6 and 17-7.) The aromatic ring in A should be highly activated with respect to nucleophilic attack, because it bears four inductively electron-withdrawing fl uorine substitu- ents, in addition to the resonance-based electron-withdrawing carbonyl group. To propose a mechanism, let’s look at the individual steps. Solution • In the conversion of A to B, the strong base Na1H2 is employed. Where are the acidic sites in A? Most obvious is the hydroxy function (pKa , 15–16, Section 8-3, Table 8-2), which will certainly be deprotonated. • What about the amino group? Closer inspection of structure A shows that the nitrogen is connected via a double bond to two carbonyl moieties. Thus, the function is resonance stabilized in much the same way as an ordinary amide (but more so): F F F OH F HN H3C O š Oð ð Oð ðOð ð F F F OH F H3C O š�Oð ð Oð ð HN� F F F OH F H3C O š�Oð ð HN� Resonance in A Consequently, it should be relatively acidic, even more acidic than an amide (pKa < 22, Section 20-7). It is therefore likely that A is doubly deprotonated before attacking the benzene nucleus. Nucleophilic aromatic substitution is then readily formulated to assemble the fi rst new ring. In the scheme below, only one—the dominating—resonance form of the anion resulting from nucleophilic attack on the arene is shown, that in which the negative charge is delocalized onto the carbonyl oxygen. � š š � Oð F O F F H3C OðO N ð š š � Oð š � F O F F H3C OðO N ð š š � O šš ð Fðð š š š �� Fðð F F F F N H3C O š Oð ð O ð First Ring Closure The second nucleophilic substitution by alkoxide is unusual, inasmuch as it has to occur at a position meta to the carbonyl function. Therefore, the intermediate anion is not stabilized by resonance, but solely by inductive effects. The regioselectivity is controlled by strain: The alkoxide cannot “reach” the more favorable carbon para to the carbonyl. F O F NF OO �Oðð CH3 š š Fðð š š š �� Fðð F O N O F OO � ð ð CH3 F O NF OO O CH3 B Second Ring Closure to B The conversion of B to C features an intermolecular substitution with an amine nucleophile. Of the two carbons bearing the potential fl uo- ride leaving groups, the one para to the carbonyl is picked, because of the resonance stabilization of the resulting intermediate anion. The initial product is formed as the ammonium salt, from which the free base C is liberated by basic work-up (Chemical Highlight 21-2). š š Fðð š F O NN H N F � OO OCH3 CH3 CH3 �HF (on basic work-up) CH3 B C A A A ðð F H O NN OO ON ð ð ð ððš � � 1034 C h a p t e r 2 2 1035 Haloarenes undergo substitution through benzyne intermediates Haloarenes devoid of electron-withdrawing substituents do not undergo simple ipso substi- tution. Nevertheless, when haloarenes are treated with nucleophiles that are also strong bases, if necessary at highly elevated temperatures, they convert to products in which the halide has been replaced by the nucleophile. For example, if exposed to hot sodium hydroxide followed by neutralizing work-up, chlorobenzene furnishes phenol. Cl Chlorobenzene OH � NaCl Phenol 1. NaOH, H2O, 340°C, 150 atm 2. H�, H2O Treatment with potassium amide results in benzenamine (aniline). Cl NH2 � KCl Benzenamine (Aniline) 1. KNH2, liquid NH3 2. H�, H2O It is tempting to assume that these substitutions follow a mechanism similar to that formulated for nucleophilic aromatic ipso substitution earlier in this section. However, when the last reaction is performed with radioactively labeled chlorobenzene (14C at C1), a very curious result is obtained: Only half of the product is substituted at the labeled carbon; in the other half, the nitrogen is at the neighboring position. Cl � Chlorobenzene-1-14C 14C label KNH2, liquid NH3 �KCl NH2 Benzenamine-1-14C 50% Benzenamine-2-14C 50% NH2 Exercise 22-10 Try It Yourself Propose a mechanism for the following conversion. Considering that the fi rst step is rate deter- mining, draw a potential-energy diagram depicting the progress of the reaction. (Hint: This is a nucleophilic aromatic substitution.) NO2 NO2 O� SO2H3C O SO2�H3C REACTION 2 2 - 4 N u c l e o p h i l i c A r o m a t i c S u b s t i t u t i o n 1036 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s Direct substitution does not seem to be the mechanism of these reactions. What, then, is the answer to this puzzle? A clue is the attachment of the incoming nucleophile only at the ipso or at the ortho position relative to the leaving group. This observation can be accounted for by an initial base-induced elimination of HX from the benzene ring, a process reminiscent of the dehydrohalogenation of haloalkenes to give alkynes (Section 13-4). In the present case, elimination is not a concerted process, but rather takes place in a sequen- tial manner, deprotonation preceding the departure of the leaving group (step 1 of the mechanism shown). Both stages in step 1 are diffi cult, with the second being worse than the fi rst. Why is that? With respect to the initial anion formation, recall (Section 11-3) that the acidity of Csp2 – H is very low (pKa < 44), and the same is true in general for phenyl hydrogens. The presence of the adjacent p system of benzene does not help, because the negative charge in the phenyl anion resides in an sp2 orbital that is perpendicular to the p frame and is therefore incapable of resonance with the double bonds in the six-membered ring. Thus, deprotonation of the haloarene requires a strong base. It takes place ortho to the halogen, because the halogen’s inductive electron-withdrawing effect acidifi es this position relative to the others. Although deprotonation is not easy, the second stage of step 1, subsequent elimination of X2, is even more diffi cult because of the highly strained structure of the resulting reactive species, called 1,2-dehydrobenzene or benzyne. Step 1. Elimination occurs stepwise Step 2. Addition occurs to both strained carbons NH2 X H Phenyl anion (Intermediate) Benzyne (Reactive intermediate; not isolated) X + X− − − − − NH2 − HNH2 H — NH2− NH2 − − NH2 − − NH2 Mechanism of Nucleophilic Substitution of Simple Haloarenes NH2 NH2 H H — NH2− NH2 NH2 H pKa ~ 44 sp2 - Hybrid orbital is perpendicular to aromatic systemπ Highly strained triple bond Why is benzyne so strained? Recall that alkynes normally adopt a linearstructure, a consequence of the sp hybridization of the carbons making up the triple bond (Sec- tion 13-2). Because of benzyne’s cyclic structure, its triple bond is forced to be bent, rendering it unusually reactive. Thus, benzyne exists only as a reactive intermediate under these conditions, being rapidly attacked by any nucleophile present. For example (step 2), amide ion or even ammonia solvent can add to furnish the product benzenamine (aniline). Because the two ends of the triple bond are equivalent, addition can take place at either carbon, explaining the label distribution in the benzenamine obtained from 14C-labeled chlorobenzene. Benzyne is too reactive to be isolated and stored in a bottle, but it can be observed spectroscopically under special conditions. Irradiation of benzocyclobutenedione at 77 K ANIM ATION ANIMATED MECHANISM: Nucleophilic aromatic substitution via benzynes MECHANISM C h a p t e r 2 2 1037 (21968C) in frozen argon (m.p. 5 21898C) produces a species whose IR and UV spectra are assignable to benzyne, formed by loss of two molecules of CO. Generation of Benzyne, a Reactive Intermediate O Benzocyclobutene-1,2-dione O � 2 COhv, 77 K Although benzyne is usually represented as a cycloalkyne (Figure 22-4A), its triple bond exhibits an IR stretching frequency of 1846 cm21, intermediate between the values for nor- mal double (cyclohexene, 1652 cm21) and triple (3-hexyne, 2207 cm21) bonds. The 13C NMR values for these carbons (d 5 182.7 ppm) are also atypical of pure triple bonds (Sec tion 13-3), indicating a considerable contribution of the cumulated triene (Section 14-5) resonance form (Figure 22-4B). The bond is weakened substantially by poor p orbital overlap in the plane of the ring. C 1.43 Å 1.42 Å Poor overlap BA 1.24 Å 1.40 Å Figure 22-4 (A) The orbital picture of benzyne reveals that the six aromatic p electrons are located in orbitals that are perpendicular to the two additional hybrid orbitals making up the distorted triple bond. These hybrid orbitals overlap only poorly; therefore, benzyne is highly reactive. (B) Resonance in benzyne. (C) The electrostatic potential map of benzyne shows electron density (red) in the plane of the six-membered ring at the position of the distorted sp-hybridized carbons. Exercise 22-11 1-Chloro-4-methylbenzene (p-chlorotoluene) is not a good starting material for the preparation of 4-methylphenol (p-cresol) by direct reaction with hot NaOH, because it forms a mixture of two products. Why does it do so, and what are the two products? Exercise 22-12 Explain the regioselectivity observed in the following reaction. (Hint: Consider the effect of the methoxy group on the selectivity of attack by amide ion on the intermediate benzyne.) OCH3 Br NH2 OCH3 � H2N Major OCH3 Minor KNH2, liquid NH3 �KBr 2 2 - 4 N u c l e o p h i l i c A r o m a t i c S u b s t i t u t i o n 1038 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s Phenols are produced from arenediazonium salts The most general traditional laboratory procedure for making phenols is from arenamines through their arenediazonium salts, ArN2 1X2. Recall that primary alkanamines can be N-nitrosated but that the resulting species rearrange to diazonium ions, which are unstable — they lose nitrogen to give carbocations (Section 21-10). In contrast, primary benzenamines (anilines) are attacked by cold nitrous acid, in a reaction called diazotization, to give relatively stable, isolable, although still reactive arenediazonium salts. Compared to their alkanediazonium counterparts, these species enjoy resonance stabilization and are prevented from undergoing immediate N2 loss by the high energy of the resulting aryl cations (to be discussed in more detail in Section 22-10). When arenediazonium ions are gently heated in water, nitrogen is evolved and the resulting aryl cations are trapped extremely rapidly by the solvent to give phenols. Decomposition of Arenediazonium Salts in Water to Give Phenols R N+ N Aryl cation −N2 Δ −H+ HOH R R OH+ In these reactions, the “super” leaving group N2 accomplishes what halides are only able to do when attached to a highly electron-defi cient benzene nucleus (nucleophilic aro- matic substitution) or under extreme conditions (through benzyne intermediates), namely, replacement by hydroxide. The three mechanisms are completely different. In nucleo- philic aromatic substitution, the nucleophile attacks prior to departure of the leaving group. In the benzyne mechanism, the nucleophile acts initially as a base, followed by extrusion of the leaving group and subsequent nucleophilic attack on the strained triple bond. In the arenediazonium-ion decomposition, the leaving group exits fi rst, followed by trapping by water. The utility of this phenol synthesis is apparent when you recall that arenamines are derived from nitroarenes by reduction, and nitroarenes are made from other arenes by electrophilic aromatic substitution (Chapters 15 and 16). Therefore, retrosynthetically (Sec- tion 8-9), we can picture the hydroxy group in any position of a benzene ring that is subject to electrophilic nitration. R Diazotization NH2 R Arenediazonium ion N� N NaNO2, H�, H2O, 0°C c š C h a p t e r 2 2 1039 Retrosynthetic Connection of Phenols to Arenes NH2OH OH D(X) NH2 D(X) NO2 NO2 D(X) D(X) Donor-activated or halogen-bearing arene NH2 NO2OH A A A A Acceptor-deactivated arene Two examples are depicted below. 70% (separated from the ortho isomer by crystallization) 98% 70% NO2 BrBr NH2 Br HNO3, H2SO4 CH3COOH, Fe HNO3, H2SO4, 20�C CH3O 80% N2 �Cl� Br 4-Bromophenol (p-Bromophenol) OH Br NaNO2, HCl, H2O, 0�C NaNO2, H2SO4, H2O, 0�C H2, Ni 80�C 80�C CH3O NO2 85% CH3O NH2 82% CH3O OH CH3O N2 � HSO4� 87% 1-(3-Hydroxyphenyl)ethanone (m-Hydoxyacetophenone) Exercise 22-13 ortho-Benzenediazoniumcarboxylate A (made by diazotization of 2-aminobenzoic acid, Prob- lem 20-59) is explosive. When warmed in solution with trans,trans-2,4-hexadiene, it forms com- pound B. Explain by a mechanism. (Hint: Two other products are formed, both of which are gases.) CH3 CH3 CH3H3CN � � C O O A B B E ð Nð ðð š� q 0 % Exercise 22-14 Propose a synthesis of 4-(phenylmethyl)phenol (p-benzylphenol) from benzene. (Caution: Remember that Friedel-Crafts reactions do not work with deactivated arenes.) 2 2 - 4 N u c l e o p h i l i c A r o m a t i c S u b s t i t u t i o n 1040 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s Phenols can be made from haloarenes by Pd catalysis While we have seen that ordinary halobenzenes are resilient to reaction with hydroxide, they undergo such nucleophilic displacements in the presence of Pd salts and added phos- phine ligands PR3. X OH KOH, Pd catalyst, PR3, 100�C Pd-Catalyzed Phenol Synthesis from Haloarenes The reaction is general for substituted benzenes, providing a complement to the diazonium method described above. OCH3 Cl OCH3 OH 90% 4-Methoxyphenol (p-Methoxyphenol) 98% 1-(3-Hydroxyphenyl)ethanone (m-Hydoxyacetophenone) KOH, Pd catalyst, PR3, 100�C KOH, Pd catalyst, PR3, 100�C CH3 Br O CH3 OH O The mechanism is related to that of the Heck and other Pd-catalyzed reaction (Section 13-9; Chemical Highlight 13-1). As shown in a simplifi ed manner below, it begins by insertion of the metal into the aryl halide bond, exchange of the halide for hydroxide, and extrusion of the fi nal product with regeneration of the catalyst. Mechanism of the Pd-Catalyzed Phenol Synthesis from Haloarenes X OH X Pd OH Pd Pd HO� �X� �Pd Similar substitutions can be carried out with alkoxides to give phenol ethers, and with amines, including ammonia, to furnish benzenamines. OCH3 NH2Br 98% 3-Methoxy-N-(2-methylpropyl)benzenamine 3-Methoxy-N-(2-methylpropyl)aniline OCH3 N H � Pd catalyst, PR3, 100�C Br NH2 89% 2-(1-Methylethyl)benzenamine(o-Isopropylaniline) NH3 (14 atm), Pd catalyst, PR3, 90�C C h a p t e r 2 2 1041 In Summary When a benzene ring bears enough strongly electron-withdrawing substitu- ents, nucleophilic addition to give an intermediate anion with delocalized charge becomes feasible, followed by elimination of the leaving group (nucleophilic aromatic ipso substitu- tion). Phenols result when the nucleophile is hydroxide ion, arenamines (anilines) when it is ammonia, and alkoxyarenes when alkoxides are employed. Very strong bases are capable of eliminating HX from haloarenes to form the reactive intermediate benzynes, which are subject to nucleophilic attack to give substitution products. Phenols may also be prepared by decomposition of arenediazonium salts in water and by Pd-catalyzed hydroxylations of haloarenes. 22-5 Alcohol Chemistry of Phenols The phenol hydroxy group undergoes several of the reactions of alcohols (Chapter 9), such as protonation, Williamson ether synthesis, and esterifi cation. The oxygen in phenols is only weakly basic Phenols are not only acidic but also weakly basic. They (and their ethers) can be protonated by strong acids to give the corresponding phenyloxonium ions. Thus, as with the alkanols, the hydroxy group imparts amphoteric character (Section 8-3). However, the basicity of phenol is even less than that of the alkanols, because the lone electron pairs on the oxygen are delocalized into the benzene ring (Sections 16-1 and 16-3). The pKa values for pheny- loxonium ions are, therefore, lower than those of alkyloxonium ions. šð � OH šCH3OH � H� � H� šO š H H CH3O � i f H pKa � �6.7pKa � �2.2 H �H E pKa Values of Methyl- and Phenyloxonium Ion Exercise 22-15 How would you make the following phenols from the given starting materials? (Hint: Consult Chapters 15 and 16.) (a) OH from CF3 CF3 (b) from OCH3 NO2 NO2HO OCH3 (c) from OH 2 2 - 5 A l c o h o l C h e m i s t r y o f P h e n o l s OOH A “Green” Industrial Phenol Synthesis H3PO4Friedel-Crafts alkylation H2SO4 O2 (air), ROOR � Cheap starting materials OH � Value- added products O Although the preceding methods are valuable in the preparation of specifi cally substituted phenols, the parent compound is made industrially by the air oxidation of (1-methylethyl)- benzene (isopropylbenzene or cumene; see also Exercise 15-27) to the benzylic hydroper- oxide and its subsequent decomposition with acid (margin). The “by-product” acetone is valuable in its own right and makes this process highly cost effective, quite apart from the environmentally benign use of air as an oxidant. 1042 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s Unlike secondary and tertiary alkyloxonium ions derived from alcohols, phenyloxonium derivatives do not dissociate to form phenyl cations, because such ions have too high an energy content (see Section 22-10). The phenyl – oxygen bond in phenols is very diffi cult to break. However, after protonation of alkoxybenzenes, the bond between the alkyl group and oxygen is readily cleaved in the presence of nucleophiles such as Br2 or I2 (e.g., from HBr or HI) to give phenol and the corresponding haloalkane. �HBr, COOH OCH3 CH3Br COOH OH 90% 3-Methoxybenzoic acid (m-Methoxybenzoic acid) 3-Hydroxybenzoic acid (m-Hydroxybenzoic acid) � Alkoxybenzenes are prepared by Williamson ether synthesis The Williamson ether synthesis (Section 9-6) permits easy preparation of many alkoxy- benzenes. The phenoxide ions obtained by deprotonation of phenols (Section 22-3) are good nucleophiles. They can displace the leaving groups from haloalkanes and alkyl sulfonates. NaOH, H2O �NaBr, �HOH OH OCH2CH2CH3 CH3CH2CH2Br ClCl 63% 3-Chlorophenol (m-Chlorophenol) 1-Chloro-3-propoxybenzene (m-Chlorophenyl propyl ether) � Esterifi cation leads to phenyl alkanoates The reaction of a carboxylic acid with a phenol (Section 19-9) to form a phenyl ester is endothermic. Therefore, esterifi cation requires an activated carboxylic acid derivative, such as an acyl halide or a carboxylic anhydride. NaOH, H2O �NaCl, �HOH OH OCCH2CH3 CH3CH2CCl O 4-Methylphenol (p-Cresol) Propanoyl chloride � B O B CH3 4-Methylphenyl propanoate (p-Methylphenyl propanoate) CH3 Exercise 22-16 Why does cleavage of an alkoxybenzene by acid not produce a halobenzene and the alkanol? Exercise 22-17 Explain why, in the preparation of acetaminophen (Chemical Highlight 22-2), the amide is formed rather than the ester. (Hint: Review Section 6-8.) C h a p t e r 2 2 1043 In Summary The oxygen in phenols and alkoxybenzenes can be protonated even though it is less basic than the oxygen in the alkanols and alkoxyalkanes. Protonated phenols and their derivatives do not ionize to phenyl cations, but the ethers can be cleaved to phenols and haloalkanes by HX. Alkoxybenzenes are made by Williamson ether synthesis, aryl alkanoates by acylation. 2 2 - 5 A l c o h o l C h e m i s t r y o f P h e n o l s CHEMICAL HIGHLIGHT 22-2 Aspirin: A Phenyl Alkanoate Drug The year 1997 marked the 100th birthday of the synthesis of the acetic ester of 2-hydroxybenzoic acid (salicylic acid); namely, 2-acetyloxybenzoic acid (acetylsalicylic acid), better known as aspirin (see Section 19-13 and Chapter 16 Opening). Aspirin is the fi rst drug that was clinically tested before it was marketed, in 1899. More than 100 billion tablets of aspirin per year are taken by people throughout the world to relieve headaches and rheumatoid and other pain, to control fever, and to treat gout and arthritis. Production capacity in the United States alone is 10,000 tons per year. Salicylic acid (also called spiric acid, hence the name aspirin [“a” for acetyl]) in extracts from the bark of the willow tree or from the meadowsweet plant had been used since ancient times (see Chapter 16 Opening) to treat pain, fever, and swelling. This acid was fi rst isolated in pure form in 1829, then synthesized in the laboratory, and fi nally produced on a large scale in the nineteenth century and prescribed as an analgesic, antipyretic, and anti-infl ammatory drug. Its bitter taste and side effects, such as mouth irritation and gastric bleeding, prompted the search for better deriva- tives that resulted in the discovery of aspirin. In the body, aspirin functions as a precursor of salicylic acid, which irreversibly inhibits the enzyme cyclooxygenase. This enzyme causes the production of prostaglandins (see Chemical Highlight 11-1 and Section 19-13), molecules that in turn are infl ammatory and pain producing. In addition, one of them, thromboxane A2, aggregates blood platelets, necessary for the clotting of blood when injury occurs. This same pro- cess is, however, undesirable inside arteries, causing heart attacks or brain strokes, depending on the location of the clot. Indeed, a large study conducted in the 1980s showed that aspirin lowered the risk of heart attacks in men by almost 50% and reduced the mortality rate during an actual attack by 23%. Many other potential applications of aspirin are under investigation, such as in the treatment of pregnancy-related complications, viral infl ammation in AIDS patients, dementia, Alzheimer’s disease, and cancer. Despite its popularity, aspi- rin can have some serious side effects: It is toxic to the liver, prolongs bleeding, and causes gastric irritation. It is sus- pected as the cause of Reye’s syndrome, a condition that leads to usually fatal brain damage. Because of some of these drawbacks, many other drugs compete with aspirin, particu- larly in the analgesics market, such as naproxen, ibuprofen, and acetaminophen (see the beginning of Chapter 16). Acetaminophen, better known as Tylenol, is prepared from 4-aminophenol by acetylation. OH 2-Hydroxybenzoic acid (o-Hydroxybenzoic acid, salicylic acid) COOH 2-Acetyloxybenzoic acid (o-Acetoxybenzoic acid, acetylsalicylic acid, aspirin)CH3COCCH3, H�, � �CH3COOH O B O B OCCH3 OCOOH B Aspirin prevents heart attacks by minimizing the formation of blood clots in the coronary arteries. The photograph shows such a blood clot (orange) in the left main pulmonary artery of a patient (see also p. 904). NH2 4-Aminophenol ( p-Aminophenol) OH HNCCH3 O N-(4-Hydroxyphenyl)acetamide [N-( p-Hydroxyphenyl)acetamide, acetaminophen, Tylenol] OH CH3COCCH3, CH3COOH �CH3COOH O B O B B 1044 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s 22-6 Electrophilic Substitution of Phenols The aromatic ring in phenols is also a center of reactivity. The interaction between the OH group and the ring strongly activates the ortho and para positions toward electrophilic sub- stitution (Sections 16-1 and 16-3). For example, even dilute nitric acid causes nitration. HNO3, CHCl3, 15�C OH 61%26% 2-Nitrophenol (o-Nitrophenol) OHOH 4-Nitrophenol ( p-Nitrophenol) � NO2 NO2 Friedel-Crafts acylation of phenols is complicated by ester formation and is better car- ried out on ether derivatives of phenol (Section 16-5), as shown in the margin. Phenols are halogenated so readily that a catalyst is not required, and multiple halogena- tions are frequently observed (Section 16-3). As shown in the following reactions, tribromi- nation occurs in water at 208C, but the reaction can be controlled to produce the monohalogenation product through the use of a lower temperature and a less polar solvent. 3 Br–Br, H2O, 20�C �3 HBr Br–Br, CHCl3, 0�C �HBr OH 100% 2,4,6-TribromophenolPhenol OHOH 4-Methylphenol ( p-Cresol) but CH3 Br Br Br 80% 2-Bromo-4-methylphenol OH Br CH3 Halogenation of Phenols Electrophilic attack at the para position is frequently dominant because of steric effects. However, it is normal to obtain mixtures resulting from both ortho and para substitutions, and their compositions are highly dependent on reagents and reaction conditions. Exercise 22-18 Friedel-Crafts methylation of methoxybenzene (anisole) with chloromethane in the presence of AlCl3 gives a 2;1 ratio of ortho;para products. Treatment of methoxybenzene with 2-chloro- 2-methylpropane (tert-butyl chloride) under the same conditions furnishes only 1-methoxy-4- (1,1-dimethylethyl) benzene (p-tert-butylanisole). Explain. (Hint: Review Section 16-5.) Exercise 22-19 Working with the Concepts: Devising Synthetic Strategies Starting with Substituted Phenols Proparacaine is a local anesthetic that is used primarily to numb the eye before minor surgical procedures, such as the removal of foreign objects or stitches. Show how you would make it from 4-hydroxybenzenecarboxylic acid. O O 4-Hydroxybenzenecarboxylic acid Proparacaine H2NOH HO O N O 70% OCH3 Methoxybenzene (Anisole) � H OCH3 1-(4-Methoxyphenyl)ethanone (p-Methoxyacetophenone) C B CH3CCl O HKO CH3 AlCl3, CS2�HCl C h a p t e r 2 2 1045 Strategy Inspection of the structures of both the starting material and the product reveals three structural modifi cations: (1) An amino group is introduced into the benzene core, suggesting a nitration-reduction sequence (Section 16-5) that relies on the ortho-directing effect of the hydroxy substituent: (2) the phenol function is etherifi ed, best done by Williamson synthesis (Section 22-5): (3) the carboxy function is esterifi ed with the appropriate amino alcohol (Section 20-2). What is the best sequence in which to execute these manipulations? To answer this question, consider the possible interference of the various functions with the suggested reaction steps. Solution • Modifi cation 1 could be carried out with the starting material, resulting in the corresponding amino acid. However, “unmasking” the amino group (from its precursor nitro) early bodes trouble with steps 2 and 3, because amines are better nucleophiles than alcohols. There- fore, attempted etherifi cation of the aminated phenol will lead to amine alkylation (Section 21-5). Similarly, attempts to effect ester forma- tion in the presence of an amine function will give rise to amide generation (Section 19-10). However, early introduction of the nitro group and maintaining it as such until the other functions are protected looks good. • For modifi cation 2, Williamson ether synthesis in the presence of a carboxy group should be fi ne, because the carboxylate ion is a poorer nucleophile than phenoxide ion (Section 6-8). • The preceding protection of the phenolic function is important to the success of modifi cation 3, ester formation with the amino alcohol, especially if we want to activate the carboxy group as an acyl chloride. • Putting it all together, a possible (and, indeed, the literature) synthesis of proparacaine is as follows: O proparacaine OH HO O O2N O2N HNO3 O N O O O2N OO OH OH HO H2, Pd-C NaOH, CH3CH2CH2Cl 1. SOCl2 2. HOCH2CH2N(CH2CH3)2 • Why does the amino alcohol not react with the acyl halide at nitrogen in the last step? After all, amines are more nucleophilic than alcohols. The answer is: It does, but because the amine function is tertiary, it can only form an acyl ammonium salt. This function has reactivity similar to that of an acyl chloride. Thus, attack by the hydroxy group on the acyl ammonium carbonyl carbon wins out thermo- dynamically to give the ester in the end (Section 20-2). B HEC O R Clð NR�3ðš š B HEC O R NR3� Clð ð š� ðš š � � � B HEC O R OR��� NR�3 R��OH Exercise 22-20 Try It Yourself Device an alternative route to proparacaine from 4-hydroxybenzenecarboxylic acid, using Pd catalysis. 2 2 - 6 E l e c t r o p h i l i c S u b s t i t u t i o n o f P h e n o l s Under basic conditions, phenols can undergo electrophilic substitution, even with very mild electrophiles, through intermediate phenoxide ions. An industrially important applica- tion is the reaction with formaldehyde, which leads to o- and p-hydroxymethylation. Mech- anistically, these processes may be considered enolate condensations, much like the aldol reaction (Section 18-5). 1046 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s CH2 OH HO� �k � O � O O � � HOH PO Hydroxymethylation of Phenol ððš šš šð ðð ðð šš O H CH2O � � O H� OCH2 HOH �HO � OH CH2OH � OH CH2OHðš ðš ðððð ð š š šš šš š ðš šš ššš The initial aldol products are unstable: They dehydrate on heating, giving reactive interme- diates called quinomethanes. HO�, � �HOH OH CH2OH O CH2OH CH2 OH HO�, � �HOH O p-Quinomethane CH2 o-Quinomethane Because quinomethanes are a,b-unsaturated carbonyl compounds, they may undergo Michael additions (Section 18-11) with excess phenoxide ion. The resulting phenols can be hydroxymethylated again and the entire process repeated. Eventually, a complex phenol – formaldehyde copolymer, also called a phenolic resin (e.g., Bakelite), is formed. Their major uses are in plywood (45%), insulation (14%), molding compounds (9%), fi brous and granulated wood (9%), and laminates (8%). OH CH2 H O� CH2OH OH CH2 O CH2 O� CH2 � O O Phenolic Resin Synthesis O H CH2 OH OH H2C CH2 OH OH polymer n H2O� P� k C h a p t e r 2 2 1047 In the Kolbe*-Schmitt† reaction, phenoxide attacks carbon dioxide to furnish the salt of 2-hydroxybenzoic acid (o-hydroxybenzoic acid, salicylic acid, precursor to aspirin; see Chemical Highlight 22-2). C O �Na� OH C O NaOH, H2O, pressure � OH B O B ðð ðð O B ðð ðHšš *Professor Adolph Wilhelm Hermann Kolbe (1818–1884), University of Leipzig, Germany. †Professor Rudolf Schmitt (1830–1898), University of Dresden, Germany. Exercise 22-21 Working with the Concepts: Recognizing Phenol as an Enol Formulate a mechanism for the Kolbe-Schmitt reaction. Strategy As always, we take an inventory of the components of the reaction: starting materials, other reagents and reaction conditions, and products. Phenol is an electron-rich arene (see this and Section 16-3) and also acidic.Carbon dioxide has an electrophilic carbon that is attacked by nucleophilic carbon atoms, such as in Grignard reagents (Section 19-6). The reaction conditions are strongly basic. Finally, the product looks like that of an electrophilic ortho substitution. Solution • Under basic conditions, phenol will exist as phenoxide, which, like an enolate ion (Section 18-1), can be described by two resonance forms (see margin). • Enolate alkylations occur at carbon (Section 18-10). In analogy, you can formulate a phenoxide attack on the electrophilic carbon of CO2. Alternatively, you can think of this reaction as an elec- trophilic attack of CO2 on a highly activated benzene ring. • Finally, deprotonation occurs to regenerate the aromatic arene. O) ) O) ) O) ) p � O) p p � OH) p OH p p B B HC O) ) B C O) ) O H B E C As you will have noticed, the selectivity for ortho attack by CO2 in this process is exceptional. Although not completely understood, it may involve direction of the electrophile by the Na1 ion in the vicinity of the phenoxide negative charge. Exercise 22-22 Try It Yourself Phentolamine (as a water-soluble methanesulfonic acid salt) is an antihypertensive that has recently been introduced into dentistry: It cuts in half the time taken to recover from the numbing effect of local anesthetics. The key step in its preparation dates from 1886, the reaction shown below. What is its mechanism? (Caution: This is not a nucleophilic aromatic substitution. Hint: Think keto – enol tautomerism). � OH CH3 NH2 OH 70% Phentolamine 160�C CH3 OH N H N HN CH3 OH N Various wood products incorpo- rating Bakelite are used in the construction of houses. 2 2 - 6 E l e c t r o p h i l i c S u b s t i t u t i o n o f P h e n o l s Phenoxide ion O � ) ) O) ) p ½ � 1048 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s In Summary The benzene ring in phenols is subject to electrophilic aromatic substitu- tion, particularly under basic conditions. Phenoxide ions can be hydroxymethylated and carbonated. 22-7 An Electrocyclic Reaction of the Benzene Ring: The Claisen Rearrangement At 2008C, 2-propenyloxybenzene (allyl phenyl ether) undergoes an unusual reaction that leads to the rupture of the allylic ether bond: The starting material rearranges to 2-(2-propenyl)phenol (o-allylphenol). 2-Propenyloxybenzene (Allyl phenyl ether) 2-(2-Propenyl)phenol (o-Allylphenol) 75% H O CH2CH CH2E P CH2PCH2CH OH � This transformation, called the Claisen* rearrangement, is another concerted reaction with a transition state that accommodates the movement of six electrons (Sections 14-8 and 15-3). The initial intermediate is a high-energy isomer, 6-(2-propenyl)-2,4-cyclohexadienone, which enolizes to the fi nal product (Sections 18-2 and 22-3). Mechanism of the Claisen Rearrangement O OH H O 6-(2-Propenyl)- 2,4-cyclohexadienone O O O The Claisen rearrangement is general for other systems. With the nonaromatic 1- ethenyloxy- 2-propene (allyl vinyl ether), it stops at the carbonyl stage because there is no driving force for enolization. This is called the aliphatic Claisen rearrangement. Exercise 22-23 Hexachlorophene (margin) is a skin germicide formerly used in soaps. It is prepared in one step from 2,4,5-trichlorophenol and formaldehyde in the presence of sulfuric acid. How does this reac- tion proceed? (Hint: Formulate an acid-catalyzed hydroxymethylation for the fi rst step.) *Professor Ludwig Claisen (1851–1930), University of Berlin, Germany. REACTION MECHANISM Hexachlorophene Cl Cl OH OH Cl Cl Cl Cl C h a p t e r 2 2 1049 Aliphatic Claisen Rearrangement 1-Ethenyloxy-2-propene (Allyl vinyl ether) 50% 4-Pentenal 255°C H C C H NCH2 CH2 E KH H2C O A H C C H HCH2 CH2 K EN H2C O A The carbon analog of the Claisen rearrangement is called the Cope* rearrangement; it takes place in compounds containing 1,5-diene units. 178°C 3-Phenyl-1,5-hexadiene Cope Rearrangement trans-1-Phenyl-1,5-hexadiene 72% Note that all of these rearrangements are related to the electrocyclic reactions that inter- convert cis-1,3,5-hexatriene with 1,3-cyclohexadiene (see margin and Section 14-9). The only difference is the absence of a double bond connecting the terminal p bonds. 2 2 - 7 C l a i s e n R e a r r a n g e m e n t *Professor Arthur C. Cope (1909–1966), Massachusetts Institute of Technology, Cambridge. Exercise 22-24 Explain the following transformation by a mechanism. (Hint: The Cope rearrangement can be accelerated greatly if it leads to charge delocalization.) OHC HO NaOH, H2O Exercise 22-25 Working with the Concepts: Applying Claisen and Cope Rearrangements Citral, B, is a component of lemon grass and as such used in perfumery (lemon and verbena scents). It is also an important intermediate in the BASF synthesis of vitamin A (Section 14-7, Chemical Highlight 18-3). The last step in the synthesis of citral requires simply heating the enol ether A. How do you get from A to B? H O B Citral O A � Electrocyclic Reaction of cis-1,3,5-Hexatriene � Strategy As always, we take an inventory of the components of the reaction: starting materials, other reagents and reaction conditions, and products. Here, this is straightforward: There are no reagents, we simply apply heat, and it appears that the reaction is an isomerization. You need to confi rm this suspicion, by determining the molecular formulas of A and B. Indeed, it is C10H16O for both. What thermal reactions could you envisage for A? Solution • You note that A contains a diene unit connected to an isolated double bond. Hence, in principle, an intramolecular Diels-Alder reaction to C might be feasible (Section 14-8; Exercise 14-24): Potential Diels-Alder Reaction of A C O A O • It is apparent that this pathway is unfavorable for two reasons: (1) Both the diene and the dieno- phile are electron rich and therefore not good partners for cycloaddition (Section 14-8), and, even more obvious, (2) a strained ring is formed in C. • An alternative is based on the recognition of a 1,5-hexadiene unit, the prerequisite for a Cope rearrangement. In A, this diene unit contains an oxygen, hence we can write a Claisen rearrange- ment to see where it leads us. DA � H O Claisen Rearrangement of A O • The product D contains a 1,5-diene substructure, capable of a Cope rearrangement, leading to citral B. BD �O H H O Cope Rearrangement of D Exercise 22-26 Try It Yourself The ether A provides B upon heating to 2008C. Formulate a mechanism. (Caution: The terminal alkenyl carbon cannot reach the para position of the benzene ring. Hint: Start with the fi rst step of a Claisen rearrangement.) OH CH3H3C O CH3H3C BA � 1050 C h a p t e r 2 2 1051 In Summary 2-Propenyloxybenzene rearranges to 2-(2-propenyl)phenol (o-allylphenol) by an electrocyclic mechanism that moves six electrons (Claisen rearrangement). Similar con- certed reactions are undergone by aliphatic unsaturated ethers (aliphatic Claisen rearrange- ment) and by hydrocarbons containing 1,5-diene units (Cope rearrangement). 22-8 Oxidation of Phenols: Benzoquinones Phenols can be oxidized to carbonyl derivatives by one-electron transfer mechanisms, result- ing in a new class of cyclic diketones, called benzoquinones. Benzoquinones and benzenediols are redox couples The phenols 1,2- and 1,4-benzenediol (for which the respective common names cat echol and hydroquinone are retained by IUPAC) are oxidized to the corresponding diketones, ortho- and para-benzoquinone, by a variety of oxidizing agents, such as sodium dichro- mate or silver oxide. Yields can be variable when the resulting diones are reactive, as in the case of o-benzoquinone, which partly decomposes under the conditions of its formation. Benzoquinones from Oxidation of Benzenediols OH OH O O Low yield Ag2O, (CH3CH2)2O OH OH O 92% O Na2Cr2O7, H2SO4 Hydroquinone p-Benzoquinone OO O O Catechol o-Benzoquinone O O O O The redox process that interconverts hydroquinone and p-benzoquinone can be visual- ized as a sequence of proton and electron transfers. Initial deprotonation gives a phenoxide ion, which is transformed into a phenoxy radical by one-electron oxidation. Proton 2 2 - 8 O x i d a t i o n o f P h e n o l s : B e n z o q u i n o n e s 1052 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s dissociation from the remaining OH group furnishes a semiquinone radical anion, and a second one-electron oxidation step leads to the benzoquinone. All of the intermediate species in this sequence benefi t from considerable resonance stabilization (two forms are shown for the semiquinone). We shall see in Section 22-9 that redox processes similar to those shown here occur widely in nature. OH OH �H� OH O � �e� Phenoxide ion OH Phenoxy radical jO �H� jO Semiquinone radical anion �e� O OjO ð ð�� ðšðšðš ðš O �ððš O �ðð�ð ðð ðð Redox Relation Between p-Benzoquinone and Hydroquinone �ð �ð Exercise 22-27 Give a minimum of two additional resonance forms each for the phenoxide ion, phenoxy radical, and semiquinone radical anion shown in the preceding scheme. The enone units in p-benzoquinones undergo conjugate and Diels-Alder additions p-Benzoquinones function as reactive a,b-unsaturated ketones in conjugate additions (see Section 18-9). For example, hydrogen chloride adds to give an intermediate hydroxy dienone that enolizes to the aromatic 2-chloro-1,4-benzenediol. O OH p-Benzoquinone O O � HCl H Cl 6-Chloro-4-hydroxy- 2,4-cyclohexadienone OH OH Cl 2-Chloro-1,4-benzenediol The double bonds also undergo cycloadditions to dienes (Section 14-8). The initial cyclo- adduct to 1,3-butadiene tautomerizes with acid to the aromatic system. � OH OH 88% overall O O C6H6, 20�C, 48 h O O H % % H HCl, � Diels–Alder Reactions of p-Benzoquinone C h a p t e r 2 2 1053 In Summary Phenols are oxidized to the corresponding benzoquinones. The diones enter into reversible redox reactions that yield the corresponding diols. They also undergo conjugate additions and Diels-Alder additions to the double bonds. 22-9 Oxidation-Reduction Processes in Nature This section describes some chemical processes involving hydroquinones and p-benzoquinones that occur in nature. We begin with an introduction to the biochemical reduction of O2. Oxygen can engage in reactions that cause damage to biomolecules. Naturally occurring antioxidants inhibit these transformations, as do several synthetic preservatives. Ubiquinones mediate the biological reduction of oxygen to water Nature makes use of the benzoquinone – hydroquinone redox couple in reversible oxidation reactions. These processes are part of the complicated cascade by which oxygen is used in biochemical degradations. An important series of compounds used for this purpose are the ubiquinones (a name coined to indicate their ubiquitous presence in nature), also collectively called coenzyme Q (CoQ, or simply Q). The ubiquinones are substituted p-benzoquinone derivatives bearing a side chain made up of 2-methylbutadiene units (isoprene; Sections 4-7 and 14-10). An enzyme system that utilizes NADH (Chemical Highlights 8-1 and 25-2) converts CoQ into its reduced form (QH2). CHEMICAL HIGHLIGHT 22-3 Chemical Warfare in Nature: The Bombardier Beetle The oxidizing power of p-benzoquinones is used by some arthropods, such as millipedes, beetles, and termites, as chemical defense agents. Most remarkable among these species is the bombardier beetle. Its name is descriptive of its defense mechanism against predators, usually ants, which involves fi ring hot corrosive chemicals from glands in its posterior, with amazing accuracy. At the time of an attack (simulated in the laboratory by pinching the beetle with fi ne- tipped forceps, see photo), two glands located near the end of the beetle’s abdomen secrete mainly hydroquinone and hydrogen peroxide, respectively, into a reaction chamber. This chamber contains enzymes that trigger the explosive oxidation of the diol to the quinone and simultaneous decomposition of hydrogen peroxide to oxygen gas and water. This cocktail is audibly expelled at temperatures up to 1008C in the direction of the enemy from the end of the beetle’s abdomen, aided for aim by a 2708 rotational capability. In some species, fi ring occurs in pulses of about 500 per second, like a machine gun. The bombardier beetle in action. Exercise 22-28 Explain the following result by a mechanism. (Hint: Review Section 18-9.) CH3 OCH3 H3C CH3O O O CH3 OCH2CH3 H3C CH3CH2O O O CH3CH2O�Na�, CH3CH2OH 2 2 - 9 O x i d a t i o n - R e d u c t i o n P r o c e s s e s i n N a t u r e 1054 C h a p t e r 2 2 C h e m i s t r y o f B e n z e n e S u b s t i t u e n t s OH OH (CH2CH CCH2)nH CH3 CH3 CH3O CH3O O Ubiquinones (n � 6, 8, 10) (Coenzyme Q) O P A (CH2CH CCH2)nH CH3 CH3 CH3O CH3O Enzyme, reducing agent Reduced form of coenzyme Q (Reduced Q, or QH2) P A QH2 participates in a chain of redox reactions with electron-transporting iron-containing proteins called cytochromes (Chemical Highlight 8-1). The reduction of Fe31 to Fe21 in cyto- chrome b by QH2 begins a sequence of electron transfers involving six different proteins. The chain ends with reduction of O2 to water by addition of four electrons and four protons. O2 1 4 H 1 1 4 e2 uy 2 H2O Phenol derivatives protect cell membranes from oxidative damage The biochemical conversion of oxygen into water includes several intermediates, including superoxide, O2 . 2, the product of one-electron reduction, and hydroxy radical, . OH, which arises from cleavage of H2O2. Both are highly reactive species capable of initiating reactions that damage organic molecules of biological importance. An example is the phosphogly- ceride shown here, a cell-membrane component derived from the unsaturated fatty acid cis,cis-octadeca-9,12-dienoic acid (linoleic acid). Initiation step R R� CH2(CH2)6CO CH2O C(CH2)14CH3 CH O O B O R� O B B A A A O CH2O PO(CH2)2N(CH3)3 O� O OH �HOH j j R R� j R Pentadienyl radical R� j C H HCH3(CH2)4 11 913 � The doubly allylic hydrogens at C11 are readily abstracted by radicals such as . OH (Section 14-2). The resonance-stabilized pentadienyl radical combines rapidly with O2 in the fi rst of two propagation steps. Reaction occurs at either C9 or C13 (shown here), giving either of two peroxy radicals containing conjugated diene units. Propagation step 1 R Peroxy radical R� j R R�O2 A jOð� Oðš C h a p t e r 2 2 1055 In the second propagation step this species removes a hydrogen atom from C11 of another molecule of phosphoglyceride, or more generally, lipid (Section 20-5), thereby forming a new dienyl radical and a molecule of lipid hydroperoxide. The dienyl radical may then reenter propagation step 1. In this way, a large number of lipid molecules may be oxidized following just a single initiation event. Propagation step 2 � � Lipid hydroperoxide R RR� R� j O OH R R� R R� A C H 11 H A jOð� Oðš ð� ðš Numerous studies have confi rmed that lipid hydroperoxides are toxic, their products of decomposition even more so. For example, loss of .OH by cleavage of the relatively weak O – O bond gives rise to an alkoxy radical, which may decompose by breaking a neighboring C – C bond (b-scission), forming an unsaturated aldehyde. R O R�H R�H O R O H OH � jOH R -Scission of a Lipid Alkoxy Radical� Alkoxy radical � jR�Oj ð� ðš � � � � ð ð Through related but more complex mechanisms, certain lipid hydroperoxides decom- pose to give unsaturated hydroxyaldehydes, such as trans-4-hydroxy-2-nonenal, as well as the dialdehyde propanedial (malondialdehyde). Molecules of these general types are partly responsible for the smell of rancid fats. Both propanedial
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