Teorico 10 Electroforesis Capilar I

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ELECTROFORESIS CAPILAR MICELAR
MEKC
SOLUTOS IONICOS
SOLUTOS NO IONICOS
SOLUTOS IONIZABLES
POLARIDAD
LIPOAFINIDAD
INTERACCIONES / AFINIDAD
BIOLOGICAL DETERGENT 
CMC 
(Mm) 
AGREGATION 
NUMBER 
MOLECULAR 
WEIGHT 
Anionic 
Cholic Acid, Sodium Salt 
Deoxycholic Acid, Sodium Salt 
Glycocholic Acid, Sodium Salt 
Glycodeoxycholic Acid, Sodium Salt 
Lauryl Sulfate, Sodium Salt 
Taurocholic Acid, Sodium Salt 
Taurodeoxycholic Acid, Sodium Salt 
14 
5 
7.1 
2.1 
8.27 
3.11 
1-4 
2-4 
4-10 
2.1 
2 
62 
4 
6 
430.6 
414.6 
487.6 
471.6 
288.4 
537.7 
521.7 
Cationic 
Hexadecyltrimethylammonium Bromide 
Tetradecyltrimethylammonium Bromide 
1 
4-5 
169 
80 
364.5 
336.4 
Zwitterionic 
N-Alkyl-N,N- dimethylammonio-1-propanesulfonates 
 SB3- 12 
 SB3- 14 
 CHAPS 
CHAPSO 
 
2- 4 
0.1-0.4 
6-10 
8 
 
55 
83 
4-14 
11 
 
335.5 
363.6 
614.9 
630.9 
Non- ionic 
BIGCHAP 
Deoxy- BIGCHAP 
Digitonin 
n- Dodecyl - d- maltoside 
n- Octyl - d- glucopyranoside 
Polyoxyethylene 23 lauryl ether ( Brij 35) 
Polyoxyethylene sorbitan monooleate (Tween 80) 
Triton X- 100 
3-4 
1.1-1.4 
- 
0.1-0.6 
20-25 
0.05-0.1 
0.012 
0.24 
10 
8-16 
60 
98 
84 
20-40 
60 
140 
878.1 
862.1 
1229.3 
510.6 
292.4 
1225 
1310 
625 
 
1. Metanol
2. Resorcinol
3. Fenol
4. P-nitroanilina
5. Nitrobenceno
6. Tolueno
7. 2-naftol
8. Sudam III
MEKC variando pH
Antipireticos analgesicos
1. Acetominophen
2. Caffeine
3. Benzamide
4. Acetanilide
5. Salicylamide
6. Acetylsalicylic
acid
7. Salycilic acid
TAA
CEFALOSPORIN
1. CTA
2. Ceftazidine
3. Cefotaxime
4. Cefmenoxime
5. Cefoperazone
6. Cefpiramide
7. Cefpimizole
8. Cefminox
9. ceftriaxone
CTAB
Ephedrine and opiates / EOF reversal
3
4
5
2
MEKC + UREA
1. Hidrocortisone
2. Hidrocortisone acetate
3. Betamethasone
4. Cortisone acetate
5. Triamcinolone acetonido
6. Fluocinolone
7. Dexamethasone acetate
8. fluocinonide
Fenol & 4-clorofenol
CD
MEKC - CD
QUIRALIDAD
MEKC
CD
AMINOINDANOL ISOMERS
SBE-CD
Micelas quirales
Sales Ac. Biliares
PTH-DL-AAs Dns-DL-AAs
1. QUINAGOLIDA
2. DOPA
MEEKC
SENSIBILIDAD
SELECTIVIDAD
ON / OFF LINE
INSTRUMENTAL
QUIMICA
AFINIDAD
• ESPECIFICA
• LIPOAFINIDAD
• OTRAS INTERACCIONES
• ANTIGENO-ANTICUERPO
AFINIDAD
Longitud: 200 m
440 m
26 m
RB: Rodamine B
DCF: Dichlorofluorescein
Cumarin dyes