Trichodinids of Nile tilapia Oreochromis niloticus in Brazil first record of Trichodinella sp.

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TRICHODINIDS OF NILE TILAPIA Oreochromis niloticus IN BRAZIL: FIRST RECORD OF 
Trichodinella sp. 
 
Inácio M. Assane*, Fabrício S. Rodrigues, Gustavo M. R. Valladão, Fernanda G. de Paula, 
Caniggia L. Andrade, Déborah O. Rodrigues, Anderson P. de Moraes, Eugénio G. de Araújo, and 
Lívia M. Pascoal 
 
Laboratory of Aquatic Organisms Pathology, LAPOA 
Aquaculture Center of São Paulo State University, CAUNESP 
14884-900, Jaboticabal, SP, Brazil 
inaciomateusassane@gmail.com 
 
Massive occurrence of trichodinids is frequently accompanied by a serious disease in fish farm. 
This study characterized morphologically species of trichodinids from gills and body of Nile tilapia 
cultivated in Brazil. Dried slides with parasites were impregnated with silver nitrate (2%). 
Morphometric characters (Table 1) and schematic drawing of the denticles were taken from 
photomicrographs (Figure 1) made from the slides. The measurements were arranged and 
presented in micrometers (µm): mean ± standard deviation. 
 
Table 1. Morphometric characters of trichodinids (measurements in micrometers). 
 
** Only one parasite of this specie was found in the present study.
 
 
 
 
 
 
 
In the present study, we found 7 trichodinids 
parasitizing the gills, being four of the genera 
Trichodina Ehrenberg, 1838; one Tripartiella 
Lom, 1959; one Paratrichodina Lom, 1963 
and one Trichodinella Srámek-Husek, 1953 
(Table 1). On the body surface, three 
specimens from genera Trichodina were 
identified (Table 1). 
 
All parasites were recently identified 
parasitizing fish in Brazil, except for 
Trichodinella sp.. This is the first record of 
parasites of this genus in this region. 
Trichodinids 
species
Site Body diameter Border membrane Adhesive disc Denticulate ring Central circle N of central ridges Span Ray Central part Blade Denticle length N of denticles Pins per denticle
Trichodina 
centrostrigeata
Gills 55.73 ± 5.02 3.71 ± 0.84 49.03 ± 5.31 26.04 ± 2.89 - 13.22 ± 1.93 12.82 ± 1.94 4.57 ± 0.98 2.42 ± 0.54 5.73 ± 0.94 4.51 ± 0.37 27.71 ± 1.00 9.33 ± 1.15
Trichodina migala Gills 76.01 ± 5.66 5.67 ± 0.50 64.71 ± 6.83 40.79 ± 5.50 - - 18.48 ± 2.02 8.30 ± 1.59 3.43 ± 0.81 6.56 ± 0.87 8.53 ± 0.48 27.00 ± 1.41 10.00 ± 0
Trichodina 
heterodentata
Gills 70.45 ± 6.06 5.43 ± 0.53 59.61 ± 5.58 39.09 ± 6.99 - - 17.64 ± 1.64 9.61 ± 1.33 2.46 ± 0.27 5.51 ± 0.52 9.00 ± 1.03 23.90 ± 2.08 10.40 ± 1.14
Trichodina 
compacta
Gills 53.09 ± 2.20 4.61 ± 0.53 44.35 ± 2.09 27.13 ± 2.13 13.11 ± 1.66 - 11.80 ± 0.87 5.38 ± 0.50 2.30 ± 0.33 4.12 ± 0.51 8.16 ± 0.51 19.00 ± 0.76 8.89 ± 1.05
Paratrichodina 
africana
Gills 25.04 ± 2.50 1.80 ± 0.19 21.24 ± 2.42 12.42 ± 1.88 - - 5.82 ± 0.48 1.88 ± 0.33 0.54 ± 0.10 3.33 ± 0.27 2.42 ± 0.39 22.17 ± 1.10 -
Tripartiella 
orthodens
Gills 27.36 ± 1.35 2.50 ± 0.31 22.36 ± 1.34 10.88 ± 0.88 - - 7.42 ± 0.73 1.91 ± 0.23 0.54 ± 0.06 4.87 ± 0.60 2.57 ± 0.33 21.44 ± 1.01 5 ± 0
Trichodinella sp. Gills 22.94 ± 2.38 2.04 ± 0.38 21.65 ± 2.84 12.01 ± 1.72 - - 4.37 ± 0.35 0.56 ± 0.08 1.3 ± 0.15 2.34 ± 0.47 2.21 ± 0.28 21 ± 1.41 -
Trichodina 
compacta
Body 54.61 ± 3.39 4.57 ± 0.47 45.41 ± 3.30 28.19 ± 2.71 13.75 ± 1.73 - 11.86 ± 0.73 4.91 ± 0.55 2.51 ± 0.32 4.43 ± 0.35 8.23 ± 0.56 19.29 ± 0.96 8.32 ± 0.48
Trichodina 
heterodentata
Body 70.10 ± 6.15 5.37 ± 0.70 59.41 ± 6.14 37.61 ± 3.92 - - 17.90 ± 1.41 9.60 ± 1.05 2.66 ± 0.32 5.55 ± 0.62 9.44 ± 1.06 23.67 ± 1.03 10.76 ± 0.90
Trichodina 
magna**
Body 91.86 7.86 75.79 49.56 - - 24.41 13.10 3.63 7.44 10.09 29.00 9.00
Figure 1. Silver impregnated adhesive discs 
and schematic drawing of Trichodina 
centrostrigata (a); T. migala (b); T. 
heterodentata (c); T. compacta (d); 
Paratrichodina africana (e); Tripartiella 
orthodens (f); Trichodinella sp. (g) and 
Trichodina magna (h) obtained in the present 
study. Scale bars: 20 μm. 
e f g 
a b c d 
h