Quantify precipitates resulting from antigen-antibody interaction. In this technique, it is necessary for the antigen molecule to be multivalent in...

Quantify precipitates resulting from antigen-antibody interaction. In this technique, it is necessary for the antigen molecule to be multivalent in terms of the number of epitopes and, preferably, the antibodies to be polyclonal. From the alternatives presented below, choose the CORRECT alternative regarding immunoprecipitation techniques.
I. Among the various physical-chemical and immunological factors that interfere with the amount of precipitate formed, the main ones are the concentration of antigen and antibody.
II. The maximum precipitation is observed when the quantities of antigen and antibody are equivalent, decreasing in the presence of excess of another component.
III. The classic precipitation curve can be obtained when the antibody at a constant concentration is added to the antigen at different concentrations.
IV. Precipitation will be maximum in the zone of equivalence or ideal proportions of antigen and antibody, and as more antigen is added, the immune complex dissolves.

a. All statements are correct.
b. Only II and IV are correct.
c. Only I and II are correct.
d. Only II, III, and IV are correct.
e. Only I, II, and III are correct.


a. All statements are correct.
b. Only II and IV are correct.
c. Only I and II are correct.
d. Only II, III, and IV are correct.
e. Only I, II, and III are correct.