What are the objectives of the semeadura por esgotamento em meios sólidos and coloração de Gram techniques? Isolation of microorganisms in pure cul...

What are the objectives of the semeadura por esgotamento em meios sólidos and coloração de Gram techniques?
Isolation of microorganisms in pure cultures
Classification of bacteria based on their cell wall characteristics
Identification of specific microorganisms present in a sample
Counting of colony-forming units (CFUs)
I - Semeadura por esgotamento is used to isolate microorganisms in pure cultures, while coloração de Gram is a differential staining method that identifies different types of bacteria based on their cell wall characteristics.
II - The semeadura por esgotamento technique is performed by diluting the sample in series and spreading it uniformly over the surface of a solid culture medium, while the coloração de Gram technique involves the application of a series of dyes to bacterial cells.
III - The objectives of semeadura por esgotamento are to isolate microorganisms in pure cultures for detailed analysis, while the objectives of coloração de Gram are to classify bacteria based on their cell wall characteristics, providing preliminary information about bacterial identification.
IV - Both techniques are widely used in microbiology laboratories and play a crucial role in the diagnosis of bacterial infections, selection of appropriate treatments, and microbiological studies in general.
a) I and II are correct.
b) II and III are correct.
c) I, III, and IV are correct.
d) All statements are correct.