What are the materials and methods used in semeadura por esgotamento em meios sólidos and coloração de Gram techniques? Solid culture media Sterile...

What are the materials and methods used in semeadura por esgotamento em meios sólidos and coloração de Gram techniques?
Solid culture media
Sterile pipettes and tips
Glass spreaders or sterile inoculation loops
Incubator
Clean and sterile glass slides
Gram staining dyes
Microscope
I - The semeadura por esgotamento technique requires solid culture media, sterile pipettes and tips, glass spreaders or sterile inoculation loops, and an incubator to provide the appropriate temperature and humidity for microbial growth.
II - The coloração de Gram technique requires clean and sterile glass slides, Gram staining dyes, and a microscope for the observation of stained samples.
III - Both techniques require adherence to good laboratory practices and specific protocols provided by the laboratory or institution.
IV - The semeadura por esgotamento technique involves preparing serial dilutions of the sample in sterile liquid media, spreading an adequate aliquot of each dilution uniformly over the surface of the solid culture medium, and incubating the plates under appropriate conditions for microbial growth.
a) I and II are correct.
b) III and IV are correct.
c) I, II, and IV are correct.
d) All statements are correct.