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Student : Marinaldo Matias da Silva Junior 
Teacher: Dr. Helinando Pequeno de Oliveira
April 2018
Fabrication of chitosan-g-poly(acrylamide)/CuS nanocomposite for
controlled drug delivery and antibacterial activity
Autores: Deepak Pathania , Divya Gupta , Shilpi Agarwal, M. Asif , Vinod Kumar Gupta 
Magazine: Materials Science and Engineering
Publication : 8 April 2016
 
Summary
References
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Introduction
Goal
Characterization methods
Materials and Methods
Results and discussion
Conclusion
Introduction
Chitosan is a linear chain biopolymer obtained by the deacetylation reaction of chitin. It is also classified as a copolymer in which 2-amino-2-deoxy-D-glucopyranose units predominate. 
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Properties of chitosan
Chitosan is a biopolymer that is easy to obtain and has several utilities that are associated with the degree of deacetylation:
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Biodegradability
Encapsulation of remedies
Reduces fat content
Biocompatible
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Polyacrylamide is synthetic polymer of acrylamide. The polymer obtained absorbs a large amount of water and is therefore a hydrogel, used as biomaterial in the controlled drug delivery system
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Nanocomposites
Nanocomposites are composed of different materials reinforced with a nanometric charge in order to confer different properties of the materials used.
Chitosan 
+
Polyacrylamide
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+ CuS nanoparticles
Medication Release System
The remedies today have immediate release of the active principle, so the controlled release system promotes:
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Stability of the drug
Absorption control
Avoids occurrence in drug variation
minimized side effects
 
The disadvantages of the controlled release system:
 pH gastrointestinal 
Enzymatic activit
Disease
Goal
The objective of this paper is to present the results of the use of nanocomposite of (chitosan / polyacrylamite) and CuS in the study of the controlled release of the drug ofloxacin in the antibacterial activity with E. coli
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Characterization methods
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Infra red with Fourier transform-FTIR
Scanning Electron Microscopy -SEM
X-ray Diffraction -DRX
Transmission Electron Microscopy - TEM
 X- ray Dispersive Energy-EDX
Materials and Methods
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Synthesis of the nanocomposite of chitosan-g-poly (acrylamide)/ CuS (CPA / CS)
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Synthesized by coprecipitation methods
Nanocomposite CPA / CS
Reaction Mixture
(CPA/CS )CuS
2g of Chitosan dissolved in 100ml of Ac 1% acetic acid under stirring and 50ml added Acrylamide 0.1 M
The CuS nanoparticles were added under Stirring for 10 min. Then placed in a microwave for 15-20 min at 15 PSI and 60 ° C
The final product was filtered and washed with water Bidistilled and dried at 50 ° C for 6 h
Drug loading and drug release behavior
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1000 mg /L de ofloxacin was prepared
with destilled water
was washed with distilled water
Inventory Solution
100 ml of ofloxacin + 750mg do nanocomposite for 24 hours
The percentage of the drug was calculated by the equation
 Eq. (1): % loading medication = (Weight of a drug in a sample / Weight of drug) x 100
 Solution
Sample
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Ofloxacin Drug
 Ofloxacin
MS=ktn
The release was studied by spectrophotometry in pH buffer medium (2.2, 7.4, 9.4) at 37 ° C
It was released at time intervals (2,4,6,8,10,12
14 and 16 hours )
Mathematical analysis of drug release
 
 Eq . (2) :
MS = sample capacity
K e n = Constants; n = 0.5 normal Ficknian diffusion
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Antibacterial Activity
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0,
Results and discussion
FTIR 
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Results and discussion
FTIR
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DRX
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SEM; TEM
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 (b) Without micrograph of CPA/CS nanocomposite
 (a) Without micrograph of CuS nanopartides 
 (c) Without micrograph of Chitosan 
 (d) Has micrograph of CPA/CS nanocompisite
EDX
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 (e) EDX pattem of CuS nanoparticles 
 (f) EDX pattem of CPA/CS nanocomposite 
Drug release
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Antimicrobian activity
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Log Reduction
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Conclusion
The combination of acrylamide chains grafted with chitosan and CuS nanoparticles can be considered in the field of drug delivery. 
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Reduction of LA drug release was studied using CPA / CS nanocomposite at different pHs. The maximum drug release was observed in acidic medium.
CPA / CS nanocomposite was successfully exploited for antibacterial activity against E. coli bacteria.
References
ARAÚJO, E. M.; HAGE JR, E.; CARVALHO, A. J. F. Compatibilização de Blendas de Poliamida 6/ABS usando os copolímeros acrílicos reativos MMA-GMA e MMA-MA. Parte 1: comportamento reológico e propriedades mecânicas das blendas. Ciência e Tecnologia, v. 13, n. 3, p. 205-211, 2003. 
BURI, P. Définition et classification des systèmes matriciels. S. T. P. Pharma., v. 3, p. 193-199, 1987. 
CHANDY, T.; SHARMA, C. P. Chitosan matrix for oral sustained delivery of ampicilin. Biomaterial, v. 12, n. 12, p. 65-70, 1993.
 
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Thanks!!!
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