DEVELOPMENT OF LOW COST BIOPRINTER AND BIOINKS BASED ON GELLAN GUN - LAPONITE FOR BIOMEDICAL APPLICATIONS_DIEGO SILVA BATISTA

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DEVELOPMENT OF LOW COST BIOPRINTER AND BIOINKS BASED ON GELLAN GUN - LAPONITE FOR BIOMEDICAL APPLICATIONS.
Diego Silva Batista1, Mayte Paredes Zaldiva1, Moliria Vieira dos Santos 2, Vera Regina Leopoldo Constantino3 and Hernane da Silva Barud1
1 – Universidade de Araraquara (UNIARA)- Araraquara - SP,
2 – Biomedical Department of Biomedical Engineering - TUFTS University, Medford-MA, United States,
3 – Universidade de São Paulo (USP), São Paulo - SP,
.
Corresponded Author
Full address – E-mail
diegonisteroy@hotmail.com
Area: ( ) Food and Agriculture (X) Medical and Pharmaceutical ( ) Multifunctional Applications
Three-dimensional (3D) bioprinting is a fusion technology that has recently gained significant attention in the biomedical field. However, commercially available bioprinting platforms can be prohibitively expensive for small research facilities, especially in an academic setting. Microextrusion is a simple and relatively inexpensive technique that presents sufficient resolution and excellent viability potential to design the printing platform, learning space for polymers for biomedical application. Gellan Gum (GG) is a microbial polysaccharide generated from the bacterium Pseudomonas elodea where it has properties like biodegradability and biocompatibility, being the most used biopolymer with gelling properties, while Laponite (LAP) is a synthetic clay frequently used to improve performance and properties of the products as rheological modifiers. In the present work was to develop a new bioink with GG / LAP gum and an economical benchtop bioprinter using microextrusion technology, modeling of depository financing model (FDM) so that it can use the bioink, serving as results for its validation of the bioprinter. The projected bioprinter was able to print hydrogels with spatial precision along the X, Y and Z axes of 0.2 mm. Hydrogels were characterized by techniques such as Scanning Electron Microscopy (SEM), thermogravimetric analysis (TGA), Fourier Transform Infrared Spectroscopy (FTIR) and Rheology, were tested where they produced prototypes that could be applied as biomaterial in regenerative medicine. Our results demonstrated that both the projected bioprinter and the bioink compound of GG / LAP have excellent properties for applications in additive manufacturing and biomedical applications.
Keywords: Gellan Gum, Laponite, Bioprinting, Bioink.