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Which of the following diagnostic tests for HIV uses the Electrochemiluminescence (EQL) method? I. The first incubation consists of mixing the use...

Which of the following diagnostic tests for HIV uses the Electrochemiluminescence (EQL) method?

I. The first incubation consists of mixing the user's sample with monoclonal antibodies anti-p24 marked with biotin, recombinant antigens specific to HIV marked with biotin, synthetic peptides specific to HIV marked with ruthenium complex, monoclonal antibodies anti-p24 marked with ruthenium complex, and recombinant antigens specific to HIV marked with ruthenium complex.
II. The presence or absence of anti-HIV-1 and/or anti-HIV-2 antibodies or p24 antigen in the sample is determined by the intensity of the chemiluminescent emission obtained compared to the cut-off point.
III. The solid phase is a cone, with the upper part coated with anti-p24 antibodies and the lower part coated with recombinant antigens and synthetic peptides of HIV-1, including those of group O and HIV-2, allowing the detection of anti-HIV antibodies.
IV. The reaction mixture is aspirated into a reading cell, where the microparticles are magnetically fixed to the surface of an electrode. The unbound elements are removed, and the application of an electric current to the electrode induces a chemiluminescent emission, which is measured by a photomultiplier.
a) I and II are correct.
b) II and III are correct.
c) III and IV are correct.
d) I, II, III, and IV are correct.

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HIV   Manual Aula 5 diagnóstico laboratorial
12 pág.

Imunologia Clínica Pontifícia Universidade Católica de GoiásPontifícia Universidade Católica de Goiás

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The diagnostic test for HIV that uses the Electrochemiluminescence (EQL) method is described in the alternatives I, II, III, and IV. Therefore, the correct answer is alternative d) I, II, III, and IV are correct.

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