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CELL-DYN EMERALD 22 AL
LABORATORY INFORMATION SYSTEM
INTERFACE SPECIFICATION
PART NO. 9159915
REVISION A
 
© 2018
Abbott Laboratories
Diagnostics Division
Abbott Park, IL 60064
NOTES
CELL-DYN Emerald 22 LIS Interface Specification Page iii
9159915A—August 2018
Table of Contents
1. OBJECTIVE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
2. REVISION STATUS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
3. SCOPE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
4. TERMINOLOGY AND ABBREVIATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
5. SPECIFICATIONS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
6. TRADEMARK STATEMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
7. GENERAL SPECIFICATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
7.0 Physical Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
7.0.1 RS232 Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
7.0.2 Ethernet connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
7.0.3 Data Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
7.0.4 Host Serial Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
7.0.5 Host Net Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
7.0.6 Communication Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
7.1 Generic Principles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
7.1.1 Frame Format. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
7.1.1.1 Frame Header . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
7.1.1.2 Frame ID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
7.1.2 Data Representation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
7.1.3 Machine Identification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
7.1.4 Transmission Control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
7.1.4.1 XON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
7.1.4.2 Handshaking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
7.1.5 Connection with HOST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
7.1.5.1 Connection request . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
7.1.6 Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
7.1.7 Format Version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
7.2 Data Format . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
7.2.1 Result (Sample) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
7.2.1.1 Sending Result Request . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
7.2.1.2 Sending Result Request Acknowledge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
7.2.1.3 Analysis Result Sending . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
7.2.1.4 Alarms List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
7.2.1.5 WBC Interpretive Message. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
7.2.1.6 RBC Interpretive Message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
7.2.1.7 PLT Interpretive Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
7.2.1.8 Lmne Matrix and Threshold Details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
7.2.1.9 Result Acknowledge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.2.1.10 Error Handling in Handshake Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.2.1.11 Next Tube Acknowledges. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.2.1.12 Emerald 22 AL Acknowledge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.2.2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.2.2.1 Sending Calibration Report Frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.2.2.2 Calibration Report Acknowledge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.2.2.3 Calibration Result Frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.2.3 QC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Page iv CELL-DYN Emerald 22 LIS Interface Specification
9159915A—August 2018
7.2.3.1 QC Result Frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
7.2.4 Precision. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
7.2.4.1 Precision Result Frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
7.2.5 Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
7.3 Control Sum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
7.3.1 Algorithmic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
7.4 Receiving a Worklist from a LIS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7.4.1 Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
7.4.2 CRC Value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
7.4.3 FILE Receipt ACKNOWLEDGMENT. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
CELL-DYN Emerald 22 AL LIS Interface Specification Page 1 of 32
9159915A—August 2018
1. OBJECTIVE
This document describes the data transmission format protocol between CELL-DYN Emerald 22 AL and a 
host Laboratory Information System (LIS).
The functionality must match the software specifications of the CELL-DYN Emerald 22 AL.
2. REVISION STATUS
3. SCOPE
This document applies to the CELL-DYN Emerald 22 AL instrument. It specifies the messages and 
behaviors of the initial version of the CELL-DYN Emerald 22 AL Host Computer Interface.
4. TERMINOLOGY AND ABBREVIATIONS
Document Control Number(s) Revision Date Section(s) Revised Pages Revised and Added
New/TUR 
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>> setdistillerparams
> setpagedeviceRelease
9159915A
August 2018 Initial release: all 
sections new.
Initial release: all sections 
new.
Table 1 — CELL-DYN Emerald 22 AL Terms
Term Description
Hand-Shake Bi-directional communication using 
acknowledgment.
Frame One or more data lines.
LIS Laboratory Information System, HOST system.
Host External Computer or data collection system.
CT Close Tube mode.
OV Other Vial.
E22AL CELL-DYN Emerald 22 AL abbreviation.
CRC Cyclic Redundancy Code.
Page 2 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
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5. SPECIFICATIONS
The Host Interface for the CELL-DYN Emerald 22 AL System is a specific format designed to transfer data 
to an external system. The system does not use the ASTM or CLIS (LIS-x) standards for communications 
with a host system. The format is text-oriented and is compatible with CSV format used by various 
Microsoft Office applications.
NOTE: The password 123 (SUPERVISOR) is necessary to access the COMMUNICATION menu.
6. TRADEMARK STATEMENTS
CELL-DYN and CELL-DYN Emerald are trademarks of Abbott Laboratories in various jurisdictions. All 
other trademarks are property of their respective owners.
Table 2 — Format Description Symbols
Symbol Comment
[CR] 0x0d, carriage return
[LF] 0x0a, line feed
[CRLF] 0x0d, 0x0a
[TAB] 0x09, tabulation
[SPACE] 0x20, space character
[ETX] 0x03, end of text
'A' 0x41, a character is in quotes
(OPT) Optional field. 
If not received, Emerald 22 AL will take a default value.
(SEND) The following frame is sent by Emerald 22 AL.
(RECEIVE) The following frame is received by Emerald 22 AL.
[MACHINE_NAME] "EMD22AL" for Emerald 22 Auto Loader
CELL-DYN Emerald 22 AL LIS Interface Specification Page 3 of 32
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7. GENERAL SPECIFICATIONS
7.0 Physical Interface
7.0.1 RS232 Connector
The CELL-DYN Emerald 22 AL provides a standard DB-9 female connector, labeled RS232C, mounted on 
the rear of the instrument. The CELL-DYN Emerald 22 AL is a DCE device.
Figure 1 RS232C Label
Table 3 lists the pins on the DB-9 connector (standard RS-232 9-pin assignments).
Table 3 — DB-9 Connector Pins
Figure 2 RS232 Port 1
Pin Description
Pin 1 Data Carrier Detect (DCD input)
Pin 2 (output) CELL-DYN Emerald 22 AL Data to Host (LIS, results)
Pin 3 (input) Host Data to CELL-DYN Emerald 22 AL
Pin 4 (output) Data Terminal Ready (DTR)
Pin 5 Signal Ground
Pin 6 Data Set Ready (DSR)
Pin 7 (input) Clear to Send (CTS input) 
(not enabled by CELL-DYN Emerald 22 AL Software)
Pin 8 (output) Request to Send (RTS output) 
(not enabled by CELL-DYN 22 AL Software)
Pin 9 (not connected)
Connector Housing Physical Ground
Page 4 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
9159915A—August 2018
7.0.2 Ethernet connector
The CELL-DYN Emerald 22 AL provides an RJ 45 connector 10/100Base, labeled with the symbol below, 
mounted on the rear of the instrument.
Figure 3 RJ 45 Label
7.0.3 Data Interface
The system can be configured to use either TCP/IP or UDP/IP over Ethernet or RS232 in the 
COMMUNICATION menu by selecting HOST SERIAL (for RS 232) or HOST NET (for TCP/IP or UDP). 
The default setting is no host.
7.0.4 Host Serial Configuration
The system can be configured for data transmission on the RS232 channel by using the 
COMMUNICATION/SERIAL PARAM. Menu. The configurable transmission parameters include data 
bits, stop bits, parity and baud rate.
• The asynchronous method of data transmission (serial by bit) is used.
• All information transmitted is in character form and is represented by 8-bit or ASCII.
• Transmitted characters consist of one (1) start bit, eight (8) data bits (least significant first), odd, even 
or no parity bit, and one (1) or two (2) stop bits.
• Parity may be selected as none, odd, or even. Default parity is None.
• The transmission speed may be selected from 1200, 9600, 19,200, 57,600, or 115,200 bits per second 
(bps).
• The Default setting is 115,200 bits per second.
• The number of bits per character is eight bits.
• Stop bit by default.
7.0.5 Host Net Configuration
• The system can be configured for data transmission via TCP/IP or UDP/IP over Ethernet by using the 
COMMUNICATION/NET. PARAM. Menu.
• The configurable transmission parameters include protocol, host IP address, host port, Emerald IP 
address, Emerald mask address, and Gateway address.
• The protocol may be set to TCP/IP in this menu. The default setting is “UDP/IP”.
• The host default IP address is 192.168.0.103. Its port is default set to 1200. Its mask is 255.255.255.0.
• The Emerald 22 AL default IP address is 192.168.0.200. The gateway default address is 192.168.0.1.
• The Emerald 22 AL port is set to 3000.
CELL-DYN Emerald 22 AL LIS Interface Specification Page 5 of 32
9159915A—August 2018
7.0.6 Communication Protocol
The following are available with RS232 only.
• Transmission control is provided in one way: XOFF/XON protocol in which the Host transmits an 
XOFF character (hex 13) to stop transmission from the System and an XON character (hex 11) to re-
start transmission.
• This is optional and not default-enabled. The option is available in COMMUNICATION/SERIAL 
PARAM. Menu with the FLOW setting.
7.1 Generic Principles
7.1.1 Frame Format
A frame contains one or more lines. A line contains one or more fields. The exchanged frames are in ASCII 
code.
All lines sent by the CELL-DYN Emerald 22 AL are terminated with [CR], even if not specified in the 
examples. In receiving, the CELL-DYN Emerald 22 AL can accept: [CR], [LF], or [CRLF] as the end line.
Each frame sent by the instrument consists of the following elements:
[FRAME HEADER] [FRAME ID] [DATA SEGMENT]
7.1.1.1 Frame Header
[FRAME HEADER] is composed of: [MACHINE_NAME]; W; X; Y[CR].
Where:
• [MACHINE_NAME] is “EMD22AL.”
• W is the instrument number (2 characters maximum).
• X is the instrument serial number (13 characters), including the '-' character (for example: 312108-
000014). 
• Y is the user login, UTF8-coded.
Here after is the list of instruments that exist..
7.1.1.2 Frame ID
Each frame sent by the host consists of the following elements: 
[FRAME ID] [DATA SEGMENT]
[FRAME ID]
Composed of KEY WORD (and optional parameters) [CR].
Table 4 — Instruments
FRAME HEADER Instrument
EMD22AL CELL-DYN Emerald 22 Autoloader Parameters
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The Frame ID allows distinguishing the type of information transmitted.
[DATA SEGMENT]
Optional according to the type of frame.
7.1.2 Data Representation
Numeric data are transmitted in fields of variable length. 
• The decimal separator is the dot. 
• The field separator is the semicolon. 
• The lines separator and the end of frame indicator is the carriage return. 
• The atomic frames are:
– Results sending request when sending result frames.
– Sending of calibration packet when sending calibration results
All the frames’ lines have to be emitted (if there is no information, the parameter is let empty). 
All the identifier key words (of frame or of parameter) are in upper-case.
The following fields (SID, PID, ID, blood type, operator, lot number) are UTF8 encoded. 
The DATE format is set to DD/MM/YYYY; the time format is set to HH:MM:SS in 24-hour format.
Table 5 — Frame ID
FRAME ID
SENDER
Information
INSTRUMENT
HOST 
(handshake enabled)
CONNECT X X Connection Test Request
ACK_CONNECT X Connection acknowledged
NAK_CONNECT X Connection acknowledged with 
failed status
RESULT_READY X Instrument ready to send result 
data
RESULT X Result data being sent
ACK_RESULT_READY X Host acknowledge: ready for result 
data
ACK_RESULT X Host acknowledge: end of result 
data receipt
CALIBRATION X Calibration report being sent
ACK_CALI X Host acknowledge: end of 
calibration report receipt
Table 6 — Maximum Field Lengths 
Field Characters
OPERATOR 10
USER 10
CELL-DYN Emerald 22 AL LIS Interface Specification Page 7 of 32
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7.1.3 Machine IdentificationIn addition to the machine name, the first two serial number digits provide the machine type as illustrated in 
Table 7. 
7.1.4 Transmission Control
7.1.4.1 XON/OFF
In order to control data flow transmission, you can use the CELL-DYN Emerald 22 AL system XON/XOFF 
option.
If the flow is set at XON/XOFF, the CELL-DYN Emerald 22 AL transmission suspends when the Host 
transmits an XOFF character (hex 13), and re-starts with an XON character (hex 11). CTS/RTS signals are 
not used.
7.1.4.2 Handshaking
Also, you can use the Handshake option. If enabled, the host must reply with an appropriate frame to the 
CELL-DYN Emerald 22 AL before an adjustable delay. Otherwise, an error message appears on screen as in 
Table 8.
LOT (CALI or QC) 8
SID 16
PID 16
ID 20
Table 7 — Machine Identification
Identification Code Machine Type
03 CELL-DYN Emerald 18 Open Tube
25 CELL-DYN Emerald 22 Open Tube
31 CELL-DYN Emerald 22 Autoloader
Table 8 — Handshake Error Messages
Error Message Comment
"HOST: SYNCHRO ERROR" The acknowledge message from the host is not the one expected.
"HOST: ACK ERROR" The host acknowledges the message with an error (NACK … receive).
The format revision is different between CELL-DYN Emerald 22 AL and the 
host.
"HOST: TIME OUT" No response from the host before the delay.
Table 6 — Maximum Field Lengths (Continued)
Field Characters
Page 8 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
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7.1.5 Connection with HOST
To communicate with a host, it is not required to establish the connection. However, an established 
communication secures the communication.
7.1.5.1 Connection request
7.1.5.1.1 Initiated by the Instrument
Figure 4 Instrument Initiated Connection Request
(SEND)
[FRAME_HEADER] = machine name; instrument number; serial number; user login [CR]
[FRAME_ID] = CONNECT; serial number; format version [CR]
(RECEIVE)
ACK_CONNECT; format version [CR] if host accepts the connection.
NACK_CONNECT; format version [CR] else if host refuses the connection.
NOTE: The format version is defined in Format Version; it is verified by CELL-DYN Emerald 22 AL if 
handshaking is used.
7.1.5.1.2 Initiated by the Host
Figure 5 Host Initiated Connection Request
(RECEIVE)
[FRAME_ID] = CONNECT; C2 format version [CR] 
(SEND)
[FRAME_HEADER] = machine name; machine number; serial number; login user [CR] 
ACK_CONNECT; serial number; format version [CR]
“EMD22AL;1;250207-000451;BILL”[CR]
 “CONNECT;250207-000451;5”[CR]
“ACK_CONNECT;5”[CR]
 
Connection request
Acknowledge if 
Instrument Host
handshake is used
Or “NAK_CONNECT;5”[CR]
“CONNECT;5”[CR]
“EMD22AL;1;310207-000451;BILL”[CR]
“ACK_CONNECT;;250207-000451;5”[CR]
Connection request
InstrumentHost
CELL-DYN Emerald 22 AL LIS Interface Specification Page 9 of 32
9159915A—August 2018
7.1.5.1.3 Instrument Disconnect
Figure 6 Instrument Disconnect
(SEND)
[FRAME_ID] = DISCONNECT; serial number [CR]
No Handshake.
7.1.6 Unit
The parameters unit is specified by the line: 
UNIT; u [CR]
If the UNIT line is missing, CELL-DYN Emerald 22 AL uses the USA unit (code 1).
CELL-DYN Emerald 22 AL sends the results in the current unit (same as the displayed and printed units).
7.1.7 Format Version
Table 10 displays the format version.
Table 9 — Unit Codes
Unit Code Unit System
1 USA = standard
2 S.I.
3 S.I. MOD
4 Other (Japanese)
Table 10 — Format Version
Version Definition of Version Content
9 Creation from E22AL format rev 09.
9 Add Frame Disconnect
 “DISCONNECT;311215-000202”[CR]
 
Instrument Logout/
Instrument Host
Switch OFF
Page 10 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
9159915A—August 2018
7.2 Data Format
7.2.1 Result (Sample)
The CELL-DYN Emerald 22 AL sends the results after the Host acknowledge occurs. This behavior avoids 
a stream to be sent with no host connected.
Figure 7 Result Sample
7.2.1.1 Sending Result Request
Used in the case of a hand-shake mode. 
(SEND)
[FRAME HEADER]
[FRAME ID] = RESULT_READY; result size in bytes[CR]
7.2.1.2 Sending Result Request Acknowledge
(RECEIVE)
ACK_RESULT_READY[CR]
[FRAME HEADER]
ACK_RESULT_READY[CR]
Sending result requested
Sending result request
Instrument Host
RESULT_READY;2470[CR]
ACK_RESULT;OK[CR]
Result acknowledge
acknowledge
Analysis result sending
DATA SEGMENT
HOST with hand-shake mode
RESULT[CR]
[FRAME HEADER]
CELL-DYN Emerald 22 AL LIS Interface Specification Page 11 of 32
9159915A—August 2018
7.2.1.3 Analysis Result Sending
NOTE: The hematologic parameters line order is subject to change between 
CELL-DYN Emerald 18, CELL-DYN Emerald 22, and CELL-DYN Emerald 22 AL. Then, use the token (in 
bold) to decode the parameter.
EMD22AL; 1; 250207-000451;BILL[CR] FRAME ID
RESULT [CR] FRAME HEADER
DATE;30/10/2007 [CR] DATA SEGMENT FOLLOWS
TIME;15:36:38 [CR] 
MODE;NORMAL [CR] 
UNIT; 1 [CR]
SEQ;352; reserved[CR]
SID;3 [CR]
PID;X28 [CR]
ID;DUPONT [CR]
TYPE;STANDARD [CR}
WBC; 11.0;A;B; 2.0; 4.0; 11.0; 15.0 [CR]( Parameter; A; B; low panic ;low ; high ;high panic )
RBC; 6.00;A;B; 2.50; 4.00; 6.20; 7.00 [CR]
HGB;15.0;A;B; 8.5;11.0;18.8;20.0 [CR]
HCT;49.0;A;B;25.0;35.0;55.0;60.0 [CR]
PLT; 320;A;B; 70; 150; 400; 500 [CR]
LYM; 3.4;A;B; 0.8; 1.0; 5.0; 5.5 [CR]
MON;13.0;A;B; 1.0; 2.0;10.0;12.0 [CR]
TEST;DIF [CR] CBC or DIF
RTYPE;1 [CR] Rack type (0 for standard RACK, 1 for Sarstedt Rack)
RACK;2 [CR] Rack ID.
POS;5 [CR] Tube pos. in rack
BIRTH;14/09/1981 [CR]
SEX;1 [CR] (0 Unknown, 1 Male, 2 Female)
PRESC; ERNESTO [CR]
LOCAT;PARIS [CR]
DRAW DATE;0 [CR] (0 for UNKNOWN or 1 for TODAY or 2 for YESTERDAY)
DRAW TIME; 12:10:00 [CR] hh:mm:ss
PATIENT COMMENT; [CR]
INFO;;;[CR] Manual association (M or blank); rerun (R or blank);
OPERATOR ; M22AL.01 [CR] operator login when analysis request occurred [CR]
PREL;CT [CR] Sampling mode (CT for Close Tube, OV for Other Vial)
CYCLE;N [CR] Cycle option (N for Normal, A for Altitude)
Page 12 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
9159915A—August 2018
NEU;13.0;A;B; 1.0; 2.0;10.0;12.0 [CR]
LYM%;30.5;A;B;15.0;25.0;50.0;55.0 [CR]
MON%;13.0;A;B; 1.0; 2.0;10.0;12.0 [CR]
NEU%;13.0;A;B; 1.0; 2.0;10.0;12.0 [CR]
MCV; 81.7;A;B; 70.0; 80.0;100.0;120.0 [CR]
MCH; 25.0;A;B; 25.0; 26.0; 34.0; 35.0 [CR]
MCHC; 30.6;A;B; 28.0; 31.0; 35.0; 36.0 [CR]
RDW; 8.1;A;B; 7.0; 10.0; 20.0; 25.0 [CR]
MPV; 7.5;A;B; 5.0; 6.0; 10.0; 12.5 [CR]
PCT; 0.4;A;B; 0.1; 0.2; 0.5; 0.6 [CR]
PDW; 9.9;A;B; 5.0; 8.0; 18.0; 25.0 [CR]
EOS;1.0;A;B; 1.0; 2.0;10.0;12.0 [CR]
BAS;1.2;A;B; 1.0; 2.0;10.0;12.0 [CR]
EOS%;3.0;A;B; 1.0; 2.0;10.0;12.0 [CR]
BAS%;5.0;A;B; 1.0; 2.0;10.0;12.0 [CR]
WBC CURVE; channel 1 value;… ;channel 128 value [CR]
WBC THRESHOLDS;S1;S2;S3 [CR]
RBC CURVE; channel 1 value;… ;channel 128 value [CR]
RBC THRESHOLDS; S1;S2 [CR]
PLT CURVE; channel 1 value;… ;channel 128 value [CR]
PLT THRESHOLDS; S1 [CR]
ALARMS;x;x;x;x;x;x;x;x;x;x;x [CR]
INTERPRETIVE_WBC;y;y;y;y;y;y;y;y;y;y;y;y [CR]
INTERPRETIVE_RBC;y;y;y;y;y;y;y;y;y;y;y;y [CR]
INTERPRETIVE_PLT;y;y;y;y;y;y;y;y;y;y;y;y [CR]
COMMENT; PCT and PDW are for Info Only (if necessary)
END_RESULT; control sum value [CR]
LMNE MATRIX; [CR]
m;m;m;m;m;m;m;m;…;m;m;m;m; [CR]
m;m;m;m;m;m;m;m;…;m;m;m;m; [CR]
…………
m;m;m;m;m;m;m;m;m;m;m;T[CR] 
[CR]
LMNE SHADE MATRIX; [CR]
m;m;m;m;m;m;m;m;…;m;m;m;m; [CR]
m;m;m;m;m;m;m;m;…;m;m;m;m; [CR]
…………
m;m;m;m;m;m;m;m;m;m;m;T[CR] 
[CR]
THRES 5D LMNE MATRIX; [CR] 
N ;S ;N ;S ;… ;S ;N ;S ;N ;S ;[CR]
N ;S ;N ;S ;… ;S ;N ;S ;N ;S ;[CR]
Compressed LMNE matrix
Variable lines number.
Compressed LMNE matrix, low level
Variable lines number.
LMNE matrix thresholds x,y coordinates.
CELL-DYN Emerald 22 AL LIS Interface Specification Page 13 of 32
9159915A—August 2018
7.2.1.3.1 Field Details
Table 11 — Sampling Mode: PREL; p
Table 12 — Cycle Option: CYCLE; o
Table 13 — INFO field: INFO; x,y
Table 14 — Alarms and Pathological Message
Table 15 — Histograms and Thresholds
Table 16 — Matrix: Emerald 22 / Emerald 22 AL
NOTE: Each line size isbelow 250 characters. Lines number is variable. 
See below for more details.
p Value Machine Type Signification
“OT” E18 / E22 (OT) Open Tube
“NO_RUN” E18 / E22 (OT) / E22 AL Unknown
“OV“ E22 AL Other vial
“CT“ E22 AL Close tube
o Value Machine Type Signification
“N“ ALL Used cycle is standard.
“A” ALL Used cycle is optimized for high altitude.
Field Value
x Nothing or M for order manually matched.
y Nothing or R for rerun.
Field Value
x Nothing or alarm.
y Nothing or interpretive message.
Field Value
S1 S2 S3 Curves thresholds ([0 to127] range).
Field Value
m compressed ASCII field, 1 to 4 characters.
N Matrix Threshold name. See below for more details.
S Matrix Threshold value. See below for more details.
Page 14 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
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7.2.1.3.2 Hematological Parameter Format
Id ; value ; flag A ; flag B ; low panic ; low ; high ; high panic [CR]
WBC; 11.0 ; A ; B ; 2.0 ; 4.0 ; 11.0 ; 15.0 [CR]
Table 17 — Hematological Parameter Format
(1) in the case field 2 is set to +++++
When IUO option is not selected, PCT and PDW parameters are not sent.
7.2.1.4 Alarms List
S-U NOT DONE 
S-UP FAIL
QC NOT DONE
QC ALERT
INS_H
L1
L5
P1
P2
P3
W_CL
R_CL
O_DF
NE
MN
ML
N1
Rank Field Possible Values
1 Parameter id WBC, RBC, ….
2 Parameter value in the 
selected unit
5.84
+ + + + + (5 pluses) if over range
-----(5 dashes) if invalid result
3 Flag A Nothing or ‘*’ if result is rejected (counting or 
measure rejection) or invalidating fault. 's' for 
different alarms (see flagging scheme).
4 Flag B Nothing
D : Higher than reportable limits (1)
‘L’ Result normal value 
‘H’ Result > panic value
5 Low panic value 2.0
6 Low value 4.0
7 High value 6.0
8 High panic value 8.0
CELL-DYN Emerald 22 AL LIS Interface Specification Page 15 of 32
9159915A—August 2018
N2
IC
LN
D_CL
7.2.1.5 WBC Interpretive Message
LEU>; 
LEU; 
LYM 
NEU> 
NEU 
BAS> 
LIC 
ALY 
ERYB
NO_INTERPRETATION;
7.2.1.6 RBC Interpretive Message
ANE 
ERY> 
MICRO 
MACRO 
ANIS> 
HYPOCR
NO_INTERPRETATION
7.2.1.7 PLT Interpretive Report
THR>
THR pucDestMatrix + (16 * 128))
{
memset(pucDestMatrix, 0, 16 * 128);// clear dest
pucDest = pucDestMatrix;
break;
}
// skip next separator 
while (*pucSrc++ != ';');
}
return((int)pucDest - (int)pucDestMatrix);// return uncompressed data size
}
This function needs that ";" is a separator for the scanf() function, usually true for most systems.
3. Uncompress then draw a matrix using a TImage 128x128 pixels component (Delphi or BC++).
The following code draws the two level lmne matrix into bitmap components. Color by population is not 
managed in the example.
static u_char ucMask[] = {0x80,0x40,0x20,0x10,0x08,0x04,0x02,0x01};
UnSqueezeMatrix( pucMat, bufferM1);// dest, src uncompress 
UnSqueezeMatrix( pucMatL, bufferM2);// dest, src uncompress
for (i = 0; i Canvas->Pixels[iX+k] [iY] = ColorLow;
else if ((ucMask[k] & ucVal) != 0)// high level 
img->Canvas->Pixels[ iX+k] [iY]= ColorHigh;
}
}
FSC (Forward Scatter)
Figure 8 Forward Scatter
4. Matrix thresholds
The thresholds are on two lines after the token “THRES 5D LMNE MATRIX ;”.
They are preceded by their names and provide the X, Y coordinates segment of the matrix: the origin is 
left, bottom, channels 0.
Table 19 — Matrix Thresholds 
Name Range Min / Max Default Value
First Line
N1X 0 / 127 15
N1Y 0 / 127 20
N2X 0 / 127 23
N2Y 0 / 127 35
LnlX 0 / 127 28
LnlY 0 / 127 50
LmnX 0 / 127 26
LmnY 0 / 127 63
LneX 0 / 127 60
LneY 0 / 127 55
 Matrix Origin
High level cells
Thresholds
Low level cells
ALL (Axial Light Losses)
Page 20 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
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EosX 0 / 127 90
LmY 0 / 127 69
ICY 0 / 127 105
NmX 0 / 127 50
RneX 0 / 127 110
Second Line
BasoX1 0 / 127 25
BasoY1 0 / 127 50
BasoX2 0 / 127 33
BasoY2 0 / 127 52
BasoX3 0 / 127 29
BasoY3 0 / 127 68
BasoX4 0 / 127 22
BasoY4 0 / 127 65
Margin Around Main Threshold
NEL 0 / 127 2
NEH 0 / 127 2
MNL 0 / 127 2
MNH 0 / 127 2
MLL 0 / 127 2
MLR 0 / 127 3
LNL 0 / 127 2
LNH 0 / 127 2
Table 19 — Matrix Thresholds (Continued)
Name Range Min / Max Default Value
CELL-DYN Emerald 22 AL LIS Interface Specification Page 21 of 32
9159915A—August 2018
Figure9 Matrix Thresholds
Figure 10 Baso Detail Position
See Baso 
Detail 
Origin 
(0,0) 
Lmn x,y 
MLH
MLL 
ICY 
N1 x,y N2 x,y Lnl x,y Lne x,y EosX 
RneX NmX
NMNL MNH NEL NEH 
LNL 
LNH
Lmy 
Page 22 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
9159915A—August 2018
Matrix and thresholds sample frame
…………..
LMNE MATRIX;
Z164;40;Z1e;1;Z13;8;Z3b;9;c0;0;20;c0;0;40;Z9;……;cc;60;40;Za;5a;80;1;
;46;c;Zb;cc;2;13;1b;e0;Zb;b9;2;27;98;70;Zb;e5;…;20;Z5;1;0;3;a2;
;5c;0;20;Z3;20;Z5;6;41;8a;53;Zc;1;40;…;Z7;23;0;58;8;
;Z3;29;68;Z7;12;82;4c;60;4;0;11;40;4;e0;Z6;…;a4;21;8a;Z4;2;0;1;
;Zf;1f;c0;Ze;3;40;Z6c;4;Z2f;4;Z1f;4;Z1f;4;Z80;T 
LMNE SHADE MATRIX;
Z183;1;Z13;8;Z3c;40;0;20;Zc;10;Z2;20;Zc;1;c0;…;70;Zb;60;2;49;c8;20;
;Zb;a1;Zf;6;8;50;6;Zc;c2;8;85;9;80;Z3;1;Za;4;…;10;10;Z8;18;88;60;
;10;Z3;40;80;1;Z6;1;40;Z4;8;Za;d1;c0;Z3;8;4;…;0;c;Z5;1a;3;50;
;Z2;81;0;20;2;Z4;2;Z2;24;80;Z6;20;Z4;2;Z2;…;Ze;10;80;Zd;8;48;Ze;8;
;20;Ze;4;88;Z1f;90;Zf;20;Ze;2c;80;Ze;10;…;40;Zdc;4;Z80;T 
THRES 5D LMNE MATRIX;
N1Y;20;N1X;15;N2Y;35;N2X;23;EosX;100;LneY;55;LneX;60;LnlY;50;LnlX;28;RneX;105;NmX;46;LmY;69;
Lmn
Y;63;LmnX;26;ICY;105;
BasoY1;60;BasoX1;26;BasoY2;64;BasoX2;30;BasoY3;70;BasoX3;29;BasoY4;65;BasoX4;20;NHH;2;NHL;2;RL
L;
2;RLR;3;NLH;2;NLL;2;HLH;2;HLL;2;
7.2.1.9 Result Acknowledge
ACK_RESULT;A;B[CR]
Where A: OK or error code, B: Nothing.
NOTE: No matter what the error code transmitted by the host, the error code is not interpreted by CELL-
DYN Emerald 22 AL.
7.2.1.10 Error Handling in Handshake Mode
In handshake mode, each result is tagged if no error is raised in the acknowledge process. If an error is ever 
raised, then the result is not tagged.
At next login, the CELL-DYN Emerald 22 AL proposes the user to automatically emit the not sent results. 
The user has then the ability to accept, to postpone or ignore (in this case the results are flagged as sent).
In non-handshake mode, the CELL-DYN Emerald 22 AL assumes that all frames were correctly sent and so 
marks all samples as ‘transmitted’.
7.2.1.11 Next Tube Acknowledges
None.
7.2.1.12 Emerald 22 AL Acknowledge
(SEND)
EMD22AL X ;Y;ACK_NEXT_FOLDER [CR]
CELL-DYN Emerald 22 AL LIS Interface Specification Page 23 of 32
9159915A—August 2018
7.2.2 Calibration
Figure 11 Calibration Example
NOTE: With Hand-shake mode, each Calibration result frame must be acknowledged before the timeout.
7.2.2.1 Sending Calibration Report Frame
EMD22AL W;X ;Y[CR]
CALIBRATION;Y;calibration date;calibration time;LOT;expiry date;creation date;time creation;Y;A ;B ;C ;D ;F ;number 
of results [CR]
WBC; target value ; margin [CR] 
RBC; target value ; tolerance [CR] 
HGB; target value ; tolerance [CR] 
MCV; target value ; tolerance [CR] 
PLT; target value ; tolerance [CR] 
END_CALI ; control sum[CR]
Where:
X : user which created the lot.
Y : user which done the calibration.
A, B, C, D and F are the calibration factors, respectively, WBC, RBC, HGB, MCV and PLT. Followed by 
the number of results used for the calibration.
When a manual calibration occurred, lot information is those of the current lot in use and the number of result 
is set to 0.
7.2.2.2 Calibration Report Acknowledge
Calibration acknowledge by the host id done by the following frame:
ACK_CALI ;[Calibration Lot];A [CR]
[FRAME HEADER]
ACK_CALI;LOT;OK[CR]
Sending calibration report
Calibration report
Host
Calibration report frame
[FRAME HEADER]
Calibration result frame
ACK_RESULT;OK[CR]
acknowledgeFor each result, repeat
Calibration results sending
Calibration Result
acknowledge
Emerald
HOST with hand shake mode
Page 24 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
9159915A—August 2018
Where A : OK or Error code.
7.2.2.3 Calibration Result Frame
Calibration result follows Calibration report. Define the number of results used for calibration and to be sent 
in the Calibration report.
Each result contains the following:
EMD22AL W;X;Y[CR] 
RESULT [CR] 
DATE;30/10/2003 [CR] 
TIME;15:36:38 [CR] 
MODE;CALIBRATION [CR] 
UNIT; Unit [CR] 
SEQ;352; 0 [CR] 
TEST;DIF [CR]
OPERATOR; login when analysis request occurred [CR]
PREL;OV[CR] 
CYCLE;N[CR]
WBC; 11.0; A;B;;;; [CR]
RBC; 6.00; A;B;;;; [CR]
HGB;15.0; A;B;;;; [CR]
MCV;49.0; A;B;;;; [CR]
PLT; 320; A;B;;;; [CR]
END_RESULT; control sum value [CR] 
A and B equal to analysis result.
7.2.3 QC
Figure 12 QC Example
[HEADER FRAME]
ACK_RESULT_READY[CR]
Sending QC result request
Sending QC result 
 Emerald Host
RESULT_READY;1280[CR]
[HEADER FRAME]
QC result frame
QC result sending
QC Result
request acknowledge
acknowledge
ACK_RESULT;OK[CR]
Host with hand shake mode
CELL-DYN Emerald 22 AL LIS Interface Specification Page 25 of 32
9159915A—August 2018
7.2.3.1 QC Result Frame
EMD22AL;W;X ;Y[CR]
RESULT [CR]
DATE;13/05/2008[CR] date then time of analysis
TIME;15:04:05[CR]
MODE;QC[CR] result type
UNIT;1[CR] used unit
SEQ;4[CR]
LOT;KDH95211[CR] lot name
LOT DATE;13/05/2008[CR] creation date
LOT TIME;15:03:19[CR] creation time
LEVEL;H[CR] lot level (1 character H, L or N)
EXPIRY DATE;04/06/2008[CR] expiry date
USER;123[CR] user (login) which created the lot
TEST;DIF[CR] DIF for DIFF result, CBC for CBC result.
OPERATOR;123[CR] operator login when analysis request occurred
PREL;OV[CR] sampling mode
CYCLE;N[CR] cycle option : N, A
WBC;8.0 ;;H;4.0 ;6.2[CR] parameter ID; value; Flag A; Flag B; target low; target high
RBC;4.30 ;;;3.80 ;5.42[CR]
HGB;11.0;;;11.0;13.2[CR]
HCT;40.0;;L;41.8;44.4[CR] 
PLT;220 ;;;210 ;232[CR]
LYM;2.1 ;;;1.3 ;2.9[CR] Only on DIF result
MON;1.6 ;;H;0.4 ;1.2[CR] Only on DIF result
NEU;3.6 ;;H;1.0 ;2.4[CR] Only on DIF result 
LYM%;26.7;;;24.0;36.2[CR] Only on DIF result 
MON%;20.0;;H;4.0 ;16.2[CR] Only on DIF result
NEU%;45.6;;L;46.0;74.2[CR] Only on DIF result 
MCV;93.0 ;;L;95.1 ;99.1[CR]
MCH;25.6 ;;H;20.3 ;23.9[CR]
MCHC;27.5 ;;;27.2 ;31.0[CR]
RDW;15.1 ;;;12.6 ;15.6[CR]
MPV;18.5 ;;H;7.3 ;10.9[CR]
PCT;0.680;;H;0.220;0.462[CR]
PDW;15.1 ;;H;10.0 ;14.2[CR]
EOS;0.6 ;;L;1.0 ;1.8[CR] Only on DIF result
BAS;0.0 ;;L;1.0 ;2.4[CR] Only on DIF result
EOS%;7.6 ;;H;2.2 ;3.0[CR] Only on DIF result
BAS%;0.1 ;;L;1.3 ;2.3[CR] Only on DIF result
END_RESULT;40579[CR] end of result; control sum value
(*) The SEQ field is filled out with the sequence number initialized by the 
CELL-DYN Emerald 22 AL when the QC run is requested.
If the QC results are sent later, then the sequence number becomes a rank number from 0 to n.
Page 26 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
9159915A—August 2018
7.2.4 Precision
Figure 13 Precision Example
[HEADER FRAME]
ACK_RESULT_READY[CR]
Sending precision
 Emerald Host
[HEADER FRAME]
Precision result frame
Precision result sending
Precision result
acknowledge
result request
ACK_RESULT;OK[CR]
RESULT_READY;2560[CR]
CELL-DYN Emerald 22 AL LIS Interface Specification Page 27 of 32
9159915A—August 2018
7.2.4.1 Precision Result Frame
[CR] are implicit for each line. The frame is similar as QC result frame, without target information.
EMD22AL;W;X ;Y[CR] [FRAME HEADER]
RESULT[CR] [FRAME ID]
DATE;13/05/2008[CR] 
TIME;15:33:09[CR]
MODE;REPEATABILITY[CR] result type
UNIT;1[CR]
SEQ;7[CR] TEST;DIF[CR]
OPERATOR;123[CR] Operator login when analysis request occurred
PREL;OV[CR] 
CYCLE;N[CR]
WBC;12.0 ;; [CR] parameter ID; value; Flag A; Flag B; target low; target high
RBC;4.70 ;; [CR]
HGB;11.4;; [CR]
HCT;40.4;; [CR]
PLT;220 ;; [CR]
LYM;0.6 ;; [CR]
MON;1.3 ;; [CR]
NEU;9.5 ;; [CR]
LYM%;5.4 ;; [CR]
MON%;11.1;; [CR]
NEU%;79.2;; [CR]
MCV;86.0 ;; [CR]
MCH;24.3 ;; [CR]
MCHC;28.2 ;; [CR]
RDW;16.2 ;; [CR]
MPV;10.4 ;; [CR]
PCT;0.380;; [CR]
PDW;14.5 ;; [CR]
EOS;0.5 ;; [CR]
BAS;0.0 ;; [CR]
EOS%;4.1 ;; [CR]
BAS%;0.2 ;; [CR]
END_RESULT;58621[CR] end of result; control sum value
Format parameters "WBC" to "BAS%" equal to analysis result.
Page 28 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
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7.2.5 Startup
EMD22AL; W;X;Y[CR] [FRAME HEADER]
STARTUP;[DATE];[TIME];[Status:PASSED/FAILED];[WBC Val];[RBC Val];[HGB Val];[PLT Val] [CR]
Example:
EMD22AL; 1;314011-000162;TEST 
STARTUP;07/11/2016;16:22:47;FAILED;0.000000;0.120000;0.100000;847.000000
7.3 Control Sum
The control sum is a CRC code: CRC-16.
This CRC is computed from the beginning sent data (including FRAME HEADER) until the end of line 
([CR] included) preceding the checksum line (identifier + value).
7.3.1 Algorithmic
The used source code is available here below (C code).
It allows generating standard CRC-16. This algorithm is mainly used in embedded systems (like hard disk 
controller). For best performance, compute is done from a seek table with 16 values.
Here is the CRC computation:
CRC = 0xFFFF
For each nibble :
Work on high weight nibble :
Index = byte EXCLUSIVE OR CRC 
Index = Index AND 000F
CRC = Table(Index) EXCLUSIVE OR (CRC divided by 16)
Work on low weight nibble : 
Index = byte divided by 16 
Index = Index EXCLUSIVE OR CRC 
Index = Index AND 000F
CRC = Table(Index) EXCLUSIVE OR (CRC divided by 16)
C language source example implementation: 
Seek table declaration:
static const unsigned short ausCrcTab1[] =
{
0x0000, 0xCC01,0xD801,0x1400,0xF001,0x3C00,0x2800,
0xE401,0xA001,0x6C00,0x7800,0xB401,0x5000,0x9C01,
0x8801,0x4400,
};
CELL-DYN Emerald 22 AL LIS Interface Specification Page 29 of 32
9159915A—August 2018
CRC computation:
unsigned short calc_crc(unsigned char *pucData, long lSize)
{
unsigned short usAcc1 = 0xFFFF;
while ( lSize > 0 )
{
/* Compute per 4 bits packages */
usAcc1 = ausCrcTab1[(*pucData ^ usAcc1) & 15] ^ (usAcc1 >> 4);
usAcc1 = ausCrcTab1[((*pucData >> 4) ^ usAcc1) &
15] ^ (usAcc1 >> 4); 
pucData++;
lSize--;
}
return(usAcc1);
}
^ : EXCLUSIVE OR
>> : logical right shift.
& : logical and.
7.4 Receiving a Worklist from a LIS
7.4.1 Principles
A LIS may connect in HOST (either NET or RS), in order to send patient files to a CELL-DYN Emerald 22 
AL. Each send is checked by the CELL-DYN Emerald 22 AL (CRC based) and acknowledged by such. If 
errors occur, they are sent back to the LIS for processing.
A LIS may transmit patient files using the "ADD_NEW_ORDER" command then processed by the CELL-
DYN Emerald 22 AL, as defined below:
ADD_NEW_ORDER,KEY,RTYPE,RACK,POS,Stat,SID,PID,ID,BIRTH,SEX,TYPE,TEST,PRESC,LOCAT,PRELD, 
PRELTIME,RUN,MATCH,Pat.COMMENT,CRC[CR]
The ADD_NEW_ORDER command is ASCII-based and must be carriage-return terminated. 
Below the parameters to be supplied:
KEY: File number. E22AL will not take this value into account, 
since it assigns a new file number for the received order. Remains at 0.
RTYPE: Rack Type number, must be : {undef, 1, .., 10 }. 
For the moment only type 0 and 1 are available. 
RACK: Rack number, must be : {undef, 1, .., 10 }
POS: Position number for the sample inside a rack, must be: {undef, 1, .., 5 }
Stat: Order Status. E22AL will not take this value into account since a
received order is considered to be done. Remains at 'T' for TODO.
SID: Order SID, 16 chars max, mandatory
PID: Order PID, 16 chars max.
Page 30 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
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ID: Order ID, 20 chars max.
BIRTH: Patient birthday with the following format DD/MM/YYYY
SEX: Patient Sex, must be: {0, 1, 2} respectively : 'undef', 'Male', 'Female'
TYPE: Blood Type, 10 chars max. STANDARD for instance. 
TEST: Test Type, must be: {0, 1} respectively, 'CBC' , 'DIF', 
PRESC: Physician Name, 20 chars max.
LOCAT: Laboratory location, 20 chars max.
PRELD: Sampling Day, must be {0,1,2} respectively 'UNKNOWN' , 
'TODAY', 'YESTERDAY'
PRELTIME: Sampling Hour with the following format HH:MM:SS
RUN: Remains blank.
MATCH: Remains blank.
Pat.COMMENT: Optional Comment, 30 chars max. 
CRC: unsigned short value containing the CRC
NOTE: Only SID and CRC fields have to be provided for the order to apply, as long as commas are 
provided even in case of blank fields.
Below, an example of an order supplied to a CELL-DYN Emerald 22 AL. This order concerns a sample 
laid inside the second slot of the first rack of type 3. The patient was born in 1990 and the sample was 
achieved by doc. House in the state of Oregon.
ADD_NEW_ORDER,0,3,1,2,T,TEST SID 1,TEST PID 1,TEST
ID,01/01/1990,1,STANDARD,1,HOUSE,OREGON,2,00:00:00,,,comment,6410\R
7.4.2 CRC Value
CRC computing (6410 in the previous example) is done from the first comma (excluded) to the last comma 
(excluded). In other words, CRC is computed using the string in yellow background.
The following CRC computing is applied:
Search array for performance efficiency.
static const unsigned short ausCrcTab1[] =
{
0x0000, 0xCC01,0xD801,0x1400,0xF001,0x3C00,0x2800,0xE401,
0xA001,0x6C00,0x7800,0xB401,0x5000,0x9C01,0x8801,0x4400,
};
CRC computing:
unsigned short calc_crc(unsigned char *pucData, long lSize)
{
unsigned short usAcc1 = 0xFFFF;
while ( lSize > 0 )
{
usAcc1 = ausCrcTab1[(*pucData ^ usAcc1) & 15] ^ (usAcc1 >> 4);
usAcc1 = ausCrcTab1[((*pucData >> 4) ^ usAcc1) & 15] ^ (usAcc1 >> 4); 
pucData++;
lSize--;
}
return(usAcc1);
}
CELL-DYN Emerald 22 AL LIS Interface Specification Page 31 of 32
9159915A—August 2018
7.4.3 FILE Receipt ACKNOWLEDGMENT
Receipt acknowledgment is done after CRC check. After a proper transmission and order processing, 
the CELL-DYN Emerald 22 AL sends back the following line to tell the LIS that no error has occurred.
ADD_NEW_ORDER: 0, OK[CR]
In case of errors, the CELL-DYN Emerald 22 AL may send back the previous command to LIS, with the error 
number followed by one of the following string:
ERR_WL_IS_FULL, Worklist is full
ERR_WL_ORDER_IS_USED, Can’t modify file because the status is not TODO. 
ERR_WL_ORDER_SID_IS_BLANK, Empty SID is not allowed
ERR_WL_ORDER_RACKPOS_USED, Rack/Position already used within the worklist. 
ERR_WL_ORDER_PID_IS_BLANK, Depending of the SETUP, file must contain a PID 
ERR_WL_ORDER_ID_IS_BLANK Depending of the SETUP, file must contain a ID
ERR_WL_ORDER_BAD_CRC CRC error.
Those strings may be parsed to detect errors and decide whether resume order sending or not. An order 
successfully sent to the CELL DYN Emerald 22 AL will not be compromised by another order that leads to 
an error.
Page 32 of 32 CELL-DYN Emerald 22 AL LIS Interface Specification
9159915A—August 2018
NOTES
	1. OBJECTIVE
	2. REVISION STATUS
	3. SCOPE
	4. TERMINOLOGY AND ABBREVIATIONS
	5. SPECIFICATIONS
	6. TRADEMARK STATEMENTS
	7. GENERAL SPECIFICATIONS
	7.0 Physical Interface
	7.0.1 RS232 Connector
	7.0.2 Ethernet connector
	7.0.3 Data Interface
	7.0.4 Host Serial Configuration
	7.0.5 Host Net Configuration
	7.0.6 Communication Protocol
	7.1 Generic Principles
	7.1.1 Frame Format
	7.1.1.1 Frame Header
	7.1.1.2 Frame ID
	7.1.2 Data Representation
	7.1.3 Machine Identification
	7.1.4 Transmission Control
	7.1.4.1 XON/OFF
	7.1.4.2 Handshaking
	7.1.5 Connection with HOST
	7.1.5.1 Connection request
	7.1.6 Unit
	7.1.7 Format Version
	7.2 Data Format
	7.2.1 Result (Sample)
	7.2.1.1 Sending Result Request
	7.2.1.2 Sending Result Request Acknowledge
	7.2.1.3 Analysis Result Sending
	7.2.1.4 Alarms List
	7.2.1.5 WBC Interpretive Message
	7.2.1.6 RBC Interpretive Message
	7.2.1.7 PLT Interpretive Report
	7.2.1.8 Lmne Matrix and Threshold Details
	7.2.1.9 Result Acknowledge
	7.2.1.10 Error Handling in Handshake Mode
	7.2.1.11 Next Tube Acknowledges
	7.2.1.12 Emerald 22 AL Acknowledge
	7.2.2 Calibration
	7.2.2.1 Sending Calibration Report Frame
	7.2.2.2 Calibration Report Acknowledge
	7.2.2.3 Calibration Result Frame
	7.2.3 QC
	7.2.3.1 QC Result Frame
	7.2.4 Precision
	7.2.4.1 Precision Result Frame
	7.2.5 Startup
	7.3 Control Sum
	7.3.1 Algorithmic
	7.4 Receiving a Worklist from a LIS
	7.4.1 Principles
	7.4.2 CRC Value
	7.4.3 FILE Receipt ACKNOWLEDGMENT
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