<div id="pf1" class="pf w0 h0" data-page-no="1"><div class="pc pc1 w0 h0"><img class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg1.png"><div class="t m0 x1 h1 y1 ff1 fs0 fc0 sc0 ls0 ws0">F<span class="_0 blank"></span>A<span class="_1 blank"></span>CULDA<span class="_1 blank"></span>DE <span class="_2 blank"> </span>DE CIÊNCIA<span class="_1 blank"></span>S <span class="_2 blank"> </span>SOCIA<span class="_1 blank"></span>IS <span class="_2 blank"> </span>E <span class="_0 blank"></span>A<span class="_1 blank"></span>PLIC<span class="_3 blank"> </span>ADA<span class="_1 blank"></span>S <span class="_2 blank"> </span> </div><div class="t m0 x2 h1 y2 ff1 fs0 fc0 sc0 ls0 ws0">F<span class="_0 blank"></span>A<span class="_1 blank"></span>CULDA<span class="_1 blank"></span>DE <span class="_2 blank"> </span>DE CIÊNCIA<span class="_1 blank"></span>S <span class="_3 blank"> </span>MÉ<span class="_3 blank"> </span>DICA<span class="_1 blank"></span>S </div><div class="t m0 x3 h1 y3 ff1 fs0 fc0 sc0 ls0 ws0">DEP<span class="_4 blank"></span>A<span class="_1 blank"></span>RT<span class="_4 blank"></span>A<span class="_1 blank"></span>MENTO <span class="_3 blank"> </span>DE BIOMEDICINA<span class="_0 blank"></span> <span class="_2 blank"> </span> </div><div class="t m0 x4 h2 y4 ff1 fs1 fc0 sc0 ls0 ws0">CURSO DE GRA<span class="_0 blank"></span>DUAÇÃO <span class="_3 blank"> </span>EM <span class="_3 blank"> </span>BIOMEDICINA<span class="_1 blank"></span> </div><div class="t m0 x5 h1 y5 ff1 fs0 fc0 sc0 ls0 ws0">DISCIPLINA<span class="_1 blank"></span>: <span class="_3 blank"> </span>Biologia <span class="_3 blank"> </span>Molecular </div><div class="t m0 x6 h1 y6 ff1 fs0 fc0 sc0 ls0 ws0"> </div><div class="t m0 x7 h3 y7 ff2 fs2 fc0 sc0 ls0 ws0">Campina<span class="_1 blank"></span> <span class="_3 blank"> </span>Grande, </div><div class="t m0 x8 h3 y8 ff2 fs2 fc0 sc0 ls0 ws0">Janeiro, 20<span class="_1 blank"></span>16 </div><div class="t m0 x9 h4 y9 ff1 fs3 fc0 sc0 ls0 ws0"> </div><div class="t m0 xa h4 ya ff1 fs3 fc0 sc0 ls0 ws0">Professora <span class="_1 blank"></span>Dra. Míriam<span class="_1 blank"></span> Goldfarb </div><div class="t m0 x9 h4 yb ff1 fs3 fc0 sc0 ls0 ws0"> </div><div class="t m0 xb h5 yc ff1 fs4 fc0 sc0 ls0 ws0">REAÇÃO<span class="_1 blank"></span> <span class="_3 blank"> </span>EM CADEIA<span class="_4 blank"></span> DA<span class="_4 blank"></span> <span class="_3 blank"></span>POLIMERA<span class="_1 blank"></span>SE </div><div class="t m0 xc h6 yd ff1 fs5 fc0 sc0 ls0 ws0">(PCR) </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf2" class="pf w0 h0" data-page-no="2"><div class="pc pc2 w0 h0"><img class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg2.png"><div class="t m0 xd h7 ye ff1 fs6 fc0 sc0 ls0 ws0">INTRODUÇÃO </div><div class="t m0 xe h6 yf ff1 fs5 fc0 sc0 ls5 ws0"> <span class="ls0"> </span></div><div class="t m0 xf h7 y10 ff3 fs6 fc1 sc0 ls1">\uf076<span class="ff1 fc0 ls0 ws0"> De<span class="_1 blank"></span>fi<span class="_3 blank"></span>nição<span class="ff2">: </span></span></div><div class="t m0 x10 h8 y11 ff2 fs7 fc0 sc0 ls0 ws0"> </div><div class="t m0 xf h9 y12 ff2 fs6 fc0 sc0 ls6 ws0"> <span class="ff4 ls0 ws1">\u201cTécnica</span><span class="ls0"> <span class="_5 blank"> </span>para <span class="_5 blank"> </span>amplificação <span class="_5 blank"> </span><span class="ls2">de</span> <span class="_5 blank"> </span>regiões <span class="_5 blank"> </span>específicas <span class="_5 blank"> </span><span class="ls7 ws2">de</span> </span></div><div class="t m0 xf h9 y13 ff2 fs6 fc0 sc0 ls0 ws0">DNA, <span class="_6 blank"> </span>utilizando <span class="_6 blank"> </span>iniciadores <span class="_6 blank"> </span>espe<span class="_3 blank"></span>cíficos <span class="_6 blank"> </span><span class="fs2 ls3"> </span>para <span class="_6 blank"> </span>determinada </div><div class="t m0 xf h9 y14 ff2 fs6 fc0 sc0 ls0 ws0">sequência e <span class="_3 blank"></span>múltiplos ciclos <span class="ls7 ws2">de</span> síntese <span class="_3 blank"></span><span class="ls7 ws2">de</span> DNA, cada ciclo </div><div class="t m0 xf h8 y15 ff2 fs7 fc0 sc0 ls0 ws0">sendo <span class="_7 blank"> </span>seguido <span class="_7 blank"> </span><span class="ls8 ws3">por</span><span class="ls4"> <span class="ls8 ws3">um</span></span> <span class="_8 blank"> </span>breve <span class="_7 blank"> </span>tra<span class="_1 blank"></span>tamento <span class="_8 blank"> </span>térmico <span class="_7 blank"> </span>para </div><div class="t m0 xf h9 y16 ff2 fs6 fc0 sc0 ls0 ws0">separar <span class="ls7 ws2">as</span> fitas <span class="ff4 ws1">complementares\u201d</span>. </div><div class="t m0 x11 h1 y17 ff1 fs0 fc0 sc0 ls0 ws0">A<span class="_1 blank"></span>lberts <span class="_3 blank"> </span> et <span class="_3 blank"> </span>al. (2010) </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf3" class="pf w0 h0" data-page-no="3"><div class="pc pc3 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg3.png"><div class="t m0 x12 h6 y18 ff1 fs6 fc0 sc0 ls0 ws1">INTRODUÇÃO<span class="fs5 ws0"> </span></div><div class="t m0 x13 h3 y19 ff2 fs2 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h7 y1a ff3 fs6 fc1 sc0 ls9">\uf076<span class="ff1 fc2 ls0 ws0"> <span class="fc0">Histórico: </span></span></div><div class="t m0 x14 ha y1b ff3 fs7 fc3 sc0 lsa">\uf0d8<span class="ff1 fc0 ls0 ws0"> <span class="ff2">W<span class="_4 blank"></span>atson e Crick, 195<span class="_1 blank"></span>3; </span></span></div><div class="t m0 x15 h9 y1c ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h9 y1d ff3 fs6 fc3 sc0 lsb">\uf0d8<span class="ff2 fc0 ls0 ws0">Dupla-fita de DNA. </span></div><div class="t m0 x14 h7 y1e ff1 fs6 fc0 sc0 lsc ws0"> <span class="ls0 v1"> </span></div><div class="t m0 x14 h7 y1f ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h7 y20 ff1 fs6 fc0 sc0 lsc ws0"> <span class="ls0 v1"> </span></div><div class="t m0 xf h7 y21 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x6 h1 y22 ff1 fs0 fc0 sc0 ls0 ws0">A<span class="_1 blank"></span>lberts <span class="_3 blank"> </span> et <span class="_3 blank"> </span>al. (2010) <span class="_3 blank"> </span>e T<span class="_4 blank"></span>obo e Medina <span class="_3 blank"> </span>(2004) </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf4" class="pf w0 h0" data-page-no="4"><div class="pc pc4 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg4.png"><div class="t m0 x16 h6 y23 ff1 fs6 fc0 sc0 ls0 ws1">INTRODUÇÃO<span class="fs5 ws0"> </span></div><div class="t m0 x13 h3 y19 ff2 fs2 fc0 sc0 ls0 ws0"> </div><div class="t m0 x17 h7 y24 ff3 fs6 fc1 sc0 ls9">\uf076<span class="ff1 fc2 ls0 ws0"> <span class="fc0">Histórico: </span></span></div><div class="t m0 x14 h9 y25 ff3 fs6 fc3 sc0 lsb">\uf0d8<span class="ff2 fc0 ls0 ws0">Nathans e Smith,1975; </span></div><div class="t m0 x15 h9 y26 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h8 y27 ff3 fs7 fc3 sc0 lsa">\uf0d8<span class="ff2 fc0 ls0 ws0">Enzimas de restrição; </span></div><div class="t m0 x15 h9 y28 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h9 y29 ff3 fs6 fc3 sc0 lsb">\uf0d8<span class="ff2 fc0 ls0 ws0">Técnicas de sequencia<span class="_1 blank"></span>me<span class="_3 blank"></span>nto, <span class="_3 blank"></span>1977; </span></div><div class="t m0 x15 h9 y2a ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h8 y2b ff3 fs7 fc3 sc0 lsa">\uf0d8<span class="ff2 fc0 ls0 ws0">Polymerase Chain Reaction (PCR), 1983; </span></div><div class="t m0 x14 h9 y2c ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h9 y2d ff3 fs6 fc3 sc0 lsb">\uf0d8<span class="ff2 fc0 ls0 ws0">Kary Mullis. </span></div><div class="t m0 x14 h7 y2e ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h7 y2f ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h7 y30 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 hb y31 ff1 fs6 fc0 sc0 lsd ws0"> <span class="fs0 ls0 v2">A<span class="_1 blank"></span>lberts <span class="_3 blank"> </span> et <span class="_3 blank"> </span>al. (2010) <span class="_3 blank"> </span>e T<span class="_4 blank"></span>obo e Medina <span class="_3 blank"> </span>(2004) </span></div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf5" class="pf w0 h0" data-page-no="5"><div class="pc pc5 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg5.png"><div class="t m0 x16 h6 y23 ff1 fs6 fc0 sc0 ls0 ws1">INTRODUÇÃO<span class="fs5 ws0"> </span></div><div class="t m0 x13 h3 y19 ff2 fs2 fc0 sc0 ls0 ws0"> </div><div class="t m0 x17 h7 y24 ff3 fs6 fc1 sc0 ls9">\uf076<span class="ff1 fc2 ls0 ws0"> <span class="fc0">Histórico </span></span></div><div class="t m0 x14 h9 y25 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h9 y26 ff3 fs6 fc3 sc0 lsb">\uf0d8<span class="ff2 fc0 ls0 ws0">Kary Mullis </span></div><div class="t m0 x14 ha y27 ff1 fs7 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h7 y28 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h7 y29 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x14 h7 y2a ff1 fs6 fc0 sc0 lsc ws0"> <span class="fs7 ls0 v1"> </span></div><div class="t m0 xf h7 y2c ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x18 h1 y32 ff1 fs0 fc0 sc0 ls0 ws0">T<span class="_4 blank"></span>obo e Medina (2004) </div><div class="t m0 x19 hc y33 ff5 fs2 fc0 sc0 ls0 ws0"> </div><div class="t m0 x19 hc y34 ff5 fs2 fc0 sc0 ls0 ws0">Propõe <span class="_9 blank"> </span>desenvolver <span class="_9 blank"> </span><span class="lsf ws4">um<span class="_1 blank"></span><span class="ls0 ws0"> <span class="_9 blank"> </span>processo </span></span></div><div class="t m0 x19 hc y35 ff5 fs2 fc0 sc0 ls10 ws5">em<span class="lse ws0"> <span class="lsf ws4">que</span><span class="ls0"> <span class="_a blank"> </span>o <span class="_a blank"> </span><span class="ls11 ws6">DNA<span class="_0 blank"></span><span class="ls0 ws0"> <span class="_b blank"> </span>poderia <span class="_a blank"> </span>ser </span></span></span></span></div><div class="t m0 x19 hc y36 ff5 fs2 fc0 sc0 ls0 ws0">multiplicado <span class="_c blank"> </span>artificialmente <span class="_c blank"> </span><span class="lsf ws4">por</span> </div><div class="t m0 x19 hd y37 ff5 fs8 fc0 sc0 ls0 ws0">meio <span class="_d blank"> </span><span class="ls12 ws7">de</span> <span class="_d blank"> </span>ciclos <span class="_d blank"> </span>repetidos <span class="_d blank"> </span><span class="ls12 ws7">de</span> </div><div class="t m0 x19 hc y38 ff5 fs2 fc0 sc0 ls0 ws0">duplicação <span class="_e blank"> </span>catalisada <span class="_e blank"> </span>pela <span class="_e blank"> </span>DNA<span class="_1 blank"></span> </div><div class="t m0 x19 hc y39 ff5 fs2 fc0 sc0 ls0 ws0">polimerase. <span class="_f blank"> </span>Reação <span class="_f blank"> </span><span class="ls10 ws5">em</span> <span class="_f blank"> </span>Cadeia <span class="_f blank"> </span><span class="lsf ws4">da</span> </div><div class="t m0 x19 hc y3a ff5 fs2 fc0 sc0 ls0 ws0">Polimerase. </div><div class="t m0 x19 hc y3b ff5 fs2 fc0 sc0 ls0 ws0"> </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf6" class="pf w0 h0" data-page-no="6"><div class="pc pc6 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg6.png"><div class="t m0 x16 h6 y23 ff1 fs6 fc0 sc0 ls0 ws1">INTRODUÇÃO<span class="fs5 ws0"> </span></div><div class="t m0 x13 h3 y19 ff2 fs2 fc0 sc0 ls0 ws0"> </div><div class="t m0 x1a ha y3c ff3 fs7 fc1 sc0 ls13">\uf076<span class="ff1 fc2 ls0 ws0"> <span class="fc0">Té<span class="_1 blank"></span>cnicas de amplificação alvos-espe<span class="_1 blank"></span>cífi<span class="_3 blank"></span>cos: </span></span></div><div class="t m0 x1b h9 y3d ff3 fs6 fc1 sc0 ls14">\uf0d8<span class="ff2 fc0 ls0 ws0"> <span class="ls10 ws8">PCR;</span> </span></div><div class="t m0 x1c h9 y3e ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x1b h8 y3f ff3 fs7 fc1 sc0 ls15">\uf0d8<span class="ff2 fc0 ls0 ws0">PCR competitiv<span class="_1 blank"></span>a; </span></div><div class="t m0 x1c h9 y40 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x1b h9 y41 ff3 fs6 fc1 sc0 ls14">\uf0d8<span class="ff2 fc0 ls10 ws8">PCR<span class="ls16">-<span class="ff6 ls0 ws0">T<span class="_10 blank"></span>ranscriptase reversa <span class="_3 blank"></span><span class="ff2">(R<span class="_1 blank"></span>T<span class="_4 blank"></span>-PC<span class="_1 blank"></span>R); </span></span></span></span></div><div class="t m0 x1c h9 y42 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x1b h9 y43 ff3 fs6 fc1 sc0 ls14">\uf0d8<span class="ff2 fc0 ls0 ws0">PCR em tempo real (qPCR<span class="ls16 ws9">).</span> </span></div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf7" class="pf w0 h0" data-page-no="7"><div class="pc pc7 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg7.png"><div class="t m0 x1d h6 y44 ff1 fs6 fc0 sc0 ls0 ws0">PRINCÍPIOS <span class="_1 blank"></span>DO M<span class="_3 blank"></span>ÉT<span class="_1 blank"></span>ODO <span class="fs5"> </span></div><div class="t m0 x1e he y45 ff2 fs8 fc0 sc0 ls0 ws0"> </div><div class="t m0 x1f h7 y46 ff3 fs6 fc4 sc0 ls9">\uf076<span class="ff1 fc0 ls0 ws0">Objetivos <span class="_1 blank"></span>principais:<span class="_3 blank"> </span> </span></div><div class="t m0 x1f h7 y47 ff1 fs6 fc2 sc0 ls0 ws0"> </div><div class="t m0 x1f h7 y48 ff3 fs6 fc4 sc0 ls17">\uf0fc<span class="ff1 fc2 ls0 ws0"> <span class="ff2 fc0 ws1">A</span><span class="ls16"> </span><span class="ff2 fc0">PC<span class="_1 blank"></span>R permite <span class="_3 blank"></span>a amplificação<span class="_1 blank"></span> <span class="_3 blank"> </span>de segmentos <span class="_3 blank"> </span>curtos da </span></span></div><div class="t m0 x20 h9 y49 ff2 fs6 fc0 sc0 ls0 ws0">molécula de DNA<span class="_11 blank"></span> (aproximadamente <span class="_3 blank"></span>100-500pb); </div><div class="t m0 x20 h9 y4a ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x1f h9 y4b ff3 fs6 fc4 sc0 ls17">\uf0fc<span class="ff2 fc0 ls0 ws0">É uma técnica<span class="_1 blank"></span> <span class="_3 blank"> </span>relativamente simples, pela qual <span class="_3 blank"> </span>a </span></div><div class="t m0 x20 h9 y4c ff2 fs6 fc0 sc0 ls0 ws0">molécula de DNA<span class="_11 blank"></span> ou <span class="_3 blank"></span>cDNA<span class="_11 blank"></span><span class="ls19"> <span class="_1 blank"></span><span class="ls0">são amplificadas <span class="_3 blank"> </span>milhares </span></span></div><div class="t m0 x20 h9 y4d ff2 fs6 fc0 sc0 ls0 ws0">ou milhões de vezes de uma forma rápida; </div><div class="t m0 x20 h8 y4e ff2 fs7 fc0 sc0 ls0 ws0"> </div><div class="t m0 x1f h9 y4f ff3 fs6 fc4 sc0 ls17">\uf0fc<span class="ff2 fc0 ls0 ws0">Amplificação de DNA<span class="_11 blank"></span> a partir de quantidade <span class="_3 blank"> </span>mínima de </span></div><div class="t m0 x20 h9 y50 ff2 fs6 fc0 sc0 ls0 ws0">amostra; </div><div class="t m0 x20 h9 y51 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x1f h8 y52 ff3 fs7 fc4 sc0 ls18">\uf0fc<span class="ff2 fc0 ls1a ws0"> <span class="ls0">Uso da técnica em pesquisa básica e aplicad<span class="_1 blank"></span>a.<span class="_3 blank"> </span> </span></span></div><div class="t m0 x21 h1 y53 ff1 fs0 fc0 sc0 ls0 ws0">Passaglia e Zaha (2004) </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf8" class="pf w0 h0" data-page-no="8"><div class="pc pc8 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg8.png"><div class="t m0 x1d h6 y44 ff1 fs6 fc0 sc0 ls0 ws0">PRINCÍPIOS <span class="_1 blank"></span>DO M<span class="_3 blank"></span>ÉT<span class="_1 blank"></span>ODO <span class="fs5"> </span></div><div class="t m0 x1e he y45 ff2 fs8 fc0 sc0 ls0 ws0"> </div><div class="t m0 x22 h7 y54 ff3 fs6 fc4 sc0 ls1b">\uf076<span class="ff1 fc0 ls0 ws0">Técnica: </span></div><div class="t m0 xf h7 y55 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x23 h7 y56 ff3 fs6 fc4 sc0 ls9">\uf076<span class="ff1 fc0 ls0 ws0">Enzimas: </span></div><div class="t m0 xf h7 y57 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x23 h7 y58 ff3 fs6 fc4 sc0 lsb">\uf0d8<span class="ff1 fc0 ls0 ws0"> <span class="ff2 ls10 ws8">DNA</span><span class="ff2">-polimerase term<span class="_3 blank"> </span>oestável, DNA-polimerase </span></span></div><div class="t m0 xf h9 y59 ff2 fs6 fc0 sc0 ls7 ws0">de <span class="ff6 ls0">Therm<span class="_1 blank"></span>us <span class="_3 blank"> </span>aquaticus <span class="ff2 ls16">(</span><span class="ws1">T<span class="_10 blank"></span>aq<span class="ff2 ls16">-<span class="ls10 ws8">DNA<span class="ls0 ws0">-polimerase); <span class="_1 blank"></span> <span class="_3 blank"></span> </span></span></span></span></span></div><div class="t m0 x23 h7 y5a ff1 fs6 fc2 sc0 ls0 ws0"> </div><div class="t m0 x23 h8 y5b ff2 fs7 fc0 sc0 ls0 ws0"> </div><div class="t m0 x24 h1 y5c ff1 fs0 fc0 sc0 ls0 ws0">A<span class="_1 blank"></span>lberts <span class="_3 blank"> </span>et <span class="_3 blank"> </span>al. (2010) <span class="_3 blank"> </span>e Passaglia e <span class="_3 blank"> </span>Zaha (2004) </div><div class="t m0 x25 h1 y5d ff1 fs0 fc0 sc0 ls0 ws0">http://w<span class="_3 blank"> </span>ww.swisseduc.ch/stromboli/perm/yellow<span class="_3 blank"> </span>stone/life-en.h<span class="_3 blank"> </span>tml. </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pf9" class="pf w0 h0" data-page-no="9"><div class="pc pc9 w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bg9.png"><div class="t m0 x26 h6 y5e ff1 fs6 fc0 sc0 ls0 ws0">PRINCÍPIOS DO MÉT<span class="_1 blank"></span>ODO <span class="_1 blank"></span><span class="fs5"> </span></div><div class="t m0 x1e he y45 ff2 fs8 fc0 sc0 ls0 ws0"> </div><div class="t m0 x27 h6 y5f ff3 fs6 fc4 sc0 ls9">\uf076<span class="ff1 fc0 ls0 ws1">Técnica<span class="fs5 ws0">: </span></span></div><div class="t m0 x23 h7 y60 ff3 fs6 fc4 sc0 ls9">\uf076<span class="ff5 fc0 ls0 ws1">Primers<span class="ff1 ws0">: </span></span></div><div class="t m0 xf h7 y61 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x23 ha y62 ff3 fs7 fc4 sc0 lsa">\uf0d8<span class="ff1 fc0 ls0 ws0"> <span class="ff2">Do<span class="_1 blank"></span>is <span class="_3 blank"></span>oligon<span class="_1 blank"></span>ucl<span class="_3 blank"></span>eotídeos <span class="_3 blank"></span>inici<span class="_1 blank"></span>adores; <span class="_3 blank"> </span> </span></span></div><div class="t m0 xf h9 y63 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x23 h7 y64 ff3 fs6 fc4 sc0 ls9">\uf076<span class="ff1 fc0 ls0 ws0">Nucleotídeos: </span></div><div class="t m0 xf h7 y65 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x23 h8 y66 ff3 fs7 fc4 sc0 lsa">\uf0d8<span class="ff2 fc0 ls0 ws0"> De<span class="_1 blank"></span>soxirribonucleotídeos <span class="_3 blank"> </span>(dNTPs<span class="ls1d wsa">);</span> </span></div><div class="t m0 x23 h9 y67 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x23 h7 y68 ff3 fs6 fc4 sc0 ls9">\uf076<span class="ff2 fc0 ls0 ws0"> <span class="ff1">T<span class="_10 blank"></span>ampão de<span class="_1 blank"></span> reação:<span class="_3 blank"></span> </span></span></div><div class="t m0 x23 h7 y69 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x23 ha y6a ff3 fs7 fc4 sc0 lsa">\uf0d8<span class="ff1 fc0 ls0 ws0"> <span class="ff2 wsb">MgC<span class="_1 blank"></span>l<span class="fs1 ls1e wsc v3">2.</span><span class="ws0"> <span class="_3 blank"> </span> <span class="_1 blank"></span> <span class="ls1f wsd">Mg<span class="fs1 ls1e wsc v4">2+</span></span> <span class="_1 blank"></span> íon <span class="_1 blank"></span><span class="ls20 wse">co<span class="ls0 ws0">-fator <span class="ls8">da </span><span class="ff6">T<span class="_10 blank"></span>aq </span></span></span></span></span></span></div><div class="t m0 x23 h7 y6b ff1 fs6 fc0 sc0 ls0 ws0"> <span class="ff2"> <span class="_1 blank"></span> </span></div><div class="t m0 x23 h7 y6c ff1 fs6 fc2 sc0 ls0 ws0"> </div><div class="t m0 x23 h9 y6d ff2 fs6 fc0 sc0 ls1c ws0"> <span class="ff1 fs0 ls0 v5">A<span class="_1 blank"></span>lberts <span class="_3 blank"> </span>et <span class="_3 blank"> </span>al. (2010) <span class="_3 blank"> </span>e Passaglia e <span class="_3 blank"> </span>Zaha (2004) </span></div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div> <div id="pfa" class="pf w0 h0" data-page-no="a"><div class="pc pca w0 h0"><img fetchpriority="low" loading="lazy" class="bi x0 y0 w0 h0" alt="" src="https://files.passeidireto.com/be12db41-e468-4ff7-ad62-e98e0225cb35/bga.png"><div class="t m0 x26 h5 y6e ff1 fs7 fc0 sc0 ls0 ws0">PRINCÍPIOS <span class="_1 blank"></span>DO M<span class="_3 blank"></span>ÉT<span class="_1 blank"></span>ODO <span class="_1 blank"></span><span class="fs4"> </span></div><div class="t m0 x1e he y45 ff2 fs8 fc0 sc0 ls0 ws0"> </div><div class="t m0 x28 h5 y6f ff3 fs7 fc4 sc0 ls13">\uf076<span class="ff1 fc0 ls0 wsb">Técnica<span class="fs4 ws0">: </span></span></div><div class="t m0 x7 h7 y70 ff1 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x29 h7 y71 ff3 fs6 fc4 sc0 ls9">\uf076<span class="ff1 fc0 ls10 ws8">DNA:<span class="ls0 ws0"> </span></span></div><div class="t m0 x29 h9 y72 ff3 fs6 fc4 sc0 lsb">\uf0d8<span class="ff2 fc0 ls0 ws0">Amostra de DN<span class="_1 blank"></span>A; <span class="_3 blank"> </span> </span></div><div class="t m0 x2a h8 y73 ff2 fs7 fc0 sc0 ls0 ws0"> </div><div class="t m0 x29 h7 y74 ff3 fs6 fc4 sc0 ls9">\uf076<span class="ff1 fc0 ls0 ws0">Água </span></div><div class="t m0 x2a h9 y75 ff2 fs6 fc0 sc0 ls0 ws0"> </div><div class="t m0 x29 h7 y76 ff1 fs6 fc2 sc0 ls0 ws0"> </div><div class="t m0 x29 h8 y77 ff2 fs7 fc0 sc0 ls0 ws0"> </div><div class="t m0 x24 h1 y78 ff1 fs0 fc0 sc0 ls0 ws0">A<span class="_1 blank"></span>lberts <span class="_3 blank"> </span>et <span class="_3 blank"> </span>al. (2010) <span class="_3 blank"> </span>e Passaglia e <span class="_3 blank"> </span>Zaha (2004) </div><div class="t m0 x23 hf y79 ff1 fs2 fc0 sc0 ls0 ws0">Reagentes para r<span class="_1 blank"></span>eação de PCR </div></div><div class="pi" data-data='{"ctm":[1.000000,0.000000,0.000000,1.000000,0.000000,0.000000]}'></div></div>
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