What are the alternatives for sexing in vitro produced bovine embryos? I - The determination of sex and viability of embryos can be achieved early...

What are the alternatives for sexing in vitro produced bovine embryos?

I - The determination of sex and viability of embryos can be achieved early and non-invasively.
II - Changes in glucose and DNP concentration, oxygen atmosphere, BCB staining and G6PD gene expression during in vitro culture of bovine embryos can affect embryonic development rates and can be used to identify embryo viability and to affect sex ratio in in vitro produced bovine embryos.
III - The sex ratio, determined by PCR, was affected by culture with 5.6 mM glucose from day 5 (D5) increased female ratio in D7 blastocysts.
IV - The atmosphere reduction to 5% O2 increased blastocyst rates, and intense blue staining of the embryos exposed to BCB on D4 was associated with higher viability.
V - Male and female two-cell and eight-cell stage embryos presented differential expression patterns for the G6PD gene.
a) I, II, III, IV and V
b) I, II, III and IV
c) II, III, IV and V
d) I, II, IV and V