What are the five steps of cloning? Cutting DNA at precise locations with restriction endonucleases, selecting a small DNA molecule capable of self...
Cutting DNA at precise locations with restriction endonucleases, selecting a small DNA molecule capable of self-replication, covalently linking two DNA fragments, moving the recombinant DNA from the test tube to a host organism, selecting or identifying host cells containing recombinant DNA
Cutting RNA at precise locations with restriction endonucleases, selecting a small RNA molecule capable of self-replication, covalently linking two RNA fragments, moving the recombinant RNA from the test tube to a host organism, selecting or identifying host cells containing recombinant RNA
Cutting DNA at precise locations with DNA polymerase, selecting a small DNA molecule capable of self-replication, covalently linking two DNA fragments, moving the recombinant DNA from the test tube to a host organism, selecting or identifying host cells containing recombinant DNA
Cutting DNA at precise locations with restriction endonucleases, selecting a small RNA molecule capable of self-replication, covalently linking two RNA fragments, moving the recombinant RNA from the test tube to a host organism, selecting or identifying host cells containing recombinant RNA
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Ed 
Os cinco passos da clonagem são: 1. Cortar o DNA em locais precisos com enzimas de restrição. 2. Selecionar uma pequena molécula de DNA capaz de auto-replicação. 3. Ligação covalente de dois fragmentos de DNA. 4. Transferir o DNA recombinante do tubo de ensaio para um organismo hospedeiro. 5. Selecionar ou identificar células hospedeiras contendo DNA recombinante. A alternativa correta é a primeira: "Cutting DNA at precise locations with restriction endonucleases, selecting a small DNA molecule capable of self-replication, covalently linking two DNA fragments, moving the recombinant DNA from the test tube to a host organism, selecting or identifying host cells containing recombinant DNA".
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