What is the mechanism of RNA synthesis catalyzed by RNAPs?
a) The reaction occurs between the 5'-OH group of a ribonucleotide and the phosphate gro...
What is the mechanism of RNA synthesis catalyzed by RNAPs? a) The reaction occurs between the 5'-OH group of a ribonucleotide and the phosphate group of the 3' carbon of the ribonucleoside triphosphate to be incorporated. b) The reaction occurs between the 3'-OH group of a ribonucleotide and the phosphate group of the 5' carbon of the ribonucleoside triphosphate to be incorporated. c) The reaction occurs between the 5'-OH group of a ribonucleotide and the phosphate group of the 5' carbon of the ribonucleoside triphosphate to be incorporated. d) The reaction occurs between the 3'-OH group of a ribonucleotide and the phosphate group of the 3' carbon of the ribonucleoside triphosphate to be incorporated. A reação catalisada pelas RNAPs é mecanisticamente idêntica à reação catalisada pelas DNA polimerases. A reação de adição ocorre no sentido 5'→3' e a fita de DNA molde (copiada) é aquela de sentido 3'→5'. O ribonucleotídeo, para ser incorporado, deve ter como característica a complementariedade ao desoxirribonucleotídeo do DNA-molde. a) The reaction occurs between the 5'-OH group of a ribonucleotide and the phosphate group of the 3' carbon of the ribonucleoside triphosphate to be incorporated. b) The reaction occurs between the 3'-OH group of a ribonucleotide and the phosphate group of the 5' carbon of the ribonucleoside triphosphate to be incorporated. c) The reaction occurs between the 5'-OH group of a ribonucleotide and the phosphate group of the 5' carbon of the ribonucleoside triphosphate to be incorporated. d) The reaction occurs between the 3'-OH group of a ribonucleotide and the phosphate group of the 3' carbon of the ribonucleoside triphosphate to be incorporated.
A alternativa correta é a letra A) A reação ocorre entre o grupo 5'-OH de um ribonucleotídeo e o grupo fosfato do carbono 3' do trifosfato de ribonucleosídeo a ser incorporado.
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