Relatorio Biotecnologia e Bioinformatica AV2

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Universidade Estácio de Sá
Biotecnologia e Bioinformática 
Curso de Biomedicina
Maria Gabriela Citeli de Matos da Costa Pinto – 202009406068
 Thamyris Marçal da Silva – 20200352336
	Eduarda –
 Samara – 
 Fernanda - 
	
 
Relatório de Bioinformática 
Relatório sobre sequenciamento biológico já existentes em bancos de dados. 
Niterói – RJ
2022 
Relatório de Bioinformática 
Relatório sobre sequenciamento biológico já existentes em bancos de dados. 
 
Relatório de atividade prática realizada no dia 28/05/2022 realizada em casa, referente ao curso Bacharelado em Biomedicina, como registro parcial desta matéria de Biotecnologia e Bioinformática.
Orientador: Professor Guilherme Lechuga.
Sumário
Introdução	3
2-	Objetivo	3
3- Resultados e discussão	3
4- Conclusão	10
Bibliografia	10
Niterói – RJ
2022
 
Introdução
Bancos de dados de sequências de genes, proteínas, estruturas e genomas. Foco no GenBank (NCBI) encontrando uma sequência de um gene de interesse A possibilidade criada pela Internet de se submeter dados on-line a um banco remoto e, de forma oposta, consultar on-line bancos de dados, incentivou a comunidade científica a centralizar em um serviço de acesso público os dados referentes a sequências de DNA e proteínas e, progressivamente, boa parte da informação gerada pelo conhecimento destas sequências.
Como a tecnologia está bem avançada na biotecnologia de análise de gene, a identificação de gene é fundamental para desenvolver uma boa pesquisa. Na busca por eles, o destaque será a INS (insulina humana) e a sequencia de outros 3 organismos.
2- Objetivo
Analisar gene da insulina INS no banco de dados national center for biotechnology information também iremos analisar filogenética, proteínas na plataforma UNIPROT e as vias metabólicas KEEG.
3- Resultados e discussão
Resultados segundo o roteiro dado pelo professor Guilherme Lechuga.
Parte 1 - Primeira parte identificar no site www.ncbi.nlm.nih.gov a sequência de gene de INS de homo sapiens. 
Encontrar a sequência INS homo sapiens:
>NC_000011.10:c2161209-2159779 Homo sapiens chromosome 11, GRCh38.p14 Primary Assembly
AGCCCTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTGTTCCAAGGGCCTTTGCGTCAGGTGGGCTCAGGATTCCAGGGTGGCTGGACCCCAGGCCCCAGCTCTGCAGCAGGGAGGACGTGGCTGGGCTCGTGAAGCATGTGGGGGTGAGCCCAGGGGCCCCAAGGCAGGGCACCTGGCCTTCAGCCTGCCTCAGCCCTGCCTGTCTCCCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGGTGAGCCAACTGCCCATTGCTGCCCCTGGCCGCCCCCAGCCACCCCCTGCTCCTGGCGCTCCCACCCAGCATGGGCAGAAGGGGGCAGGAGGCTGCCACCCAGCAGGGGGTCAGGTGCACTTTTTTAAAAAGAAGTTCTCTTGGTCACGTCCTAAAAGTGACCAGCTCCCTGTGGCCCAGTCAGAATCTCAGCCTGAGGACGGTGTTGGCTTCGGCAGCCCCGAGATACATCAGAGGGTGGGCACGCTCCTCCCTCCACTCGCCCCTCAAACAAATGCCCCGCAGCCCATTTCTCCACCCTCATTTGATGACCGCAGATTCAAGTGTTTTGTTAAGTAAAGTCCTGGGTGACCTGGGGTCACAGGGTGCCCCACGCTGCCTGCCTCTGGGCGAACACCCCATCACGCCCGGAGGAGGGCGTGGCTGCCTGCCTGAGTGGGCCAGACCCCTGTCGCCAGGCCTCACGGCAGCTCCATAGTCAGGAGATGGGGAAGATGCTGGGGACAGGCCCTGGGGAGAAGTACTGGGATCACCTGTTCAGGCTCCCACTGTGACGCTGCCCCGGGGCGGGGGAAGGAGGTGGGACATGTGGGCGTTGGGGCCTGTAGGTCCACACCCAGTGTGGGTGACCCTCCCTCTAACCTGGGTCCAGCCCGGCTGGAGATGGGTGGGAGTGCGACCTAGGGCTGGCGGGCAGGCGGGCACTGTGTCTCCCTGACTGTGTCCTCCTGTGTCCCTCTGCCTCGCCGCTGTTCCGGAACCTGCTCTGCGCGGCACGTCCTGGCAGTGGGGCAGGTGGAGCTGGGCGGGGGCCCTGGTGCAGGCAGCCTGCAGCCCTTGGCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAACTAGACGCAGCCCGCAGGCAGCCCCACACCCGCCGCCTCCTGCACCGAGAGAGATGGAATAAAGCCCTTGAACCAGC
Parte 2 
Query: NC_000011.10:c2161209-2159779 Homo sapiens chromosome 11, GRCh38.p14 Primary Assembly Query ID: lcl|Query_43125 Length: 1431
>Homo sapiens INS-IGF2 readthrough (INS-IGF2), RefSeqGene on chromosome 11
Sequence ID: NG_050578.1 Length: 39098
Range 1: 5001 to 6431
Score:2643 bits(1431), Expect:0.0, 
Identities:1431/1431(100%), Gaps:0/1431(0%), Strand: Plus/Plus
Query 1 AGCCCTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTGTTCCAAGGGCCTT 60
Sbjct 5001 ............................................................ 5060
Query 61 TGCGTCAGGTGGGCTCAGGATTCCAGGGTGGCTGGACCCCAGGCCCCAGCTCTGCAGCAG 120
Sbjct 5061 ............................................................ 5120
Query 121 GGAGGACGTGGCTGGGCTCGTGAAGCATGTGGGGGTGAGCCCAGGGGCCCCAAGGCAGGG 180
Sbjct 5121 ............................................................ 5180
Query 181 CACCTGGCCTTCAGCCTGCCTCAGCCCTGCCTGTCTCCCAGATCACTGTCCTTCTGCCAT 240
Sbjct 5181 ............................................................ 5240
Query 241 GGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCC 300
Sbjct 5241 ............................................................ 5300
Query 301 AGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT 360
Sbjct 5301 ............................................................ 5360
Query 361 AGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCT 420
Sbjct 5361 ............................................................ 5420
Query 421 GCAGGGTGAGCCAACTGCCCATTGCTGCCCCTGGCCGCCCCCAGCCACCCCCTGCTCCTG 480
Sbjct 5421 ............................................................ 5480
Query 481 GCGCTCCCACCCAGCATGGGCAGAAGGGGGCAGGAGGCTGCCACCCAGCAGGGGGTCAGG 540
Sbjct 5481 ............................................................ 5540
Query 541 TGCACTTTTTTAAAAAGAAGTTCTCTTGGTCACGTCCTAAAAGTGACCAGCTCCCTGTGG 600
Sbjct 5541 ............................................................ 5600
Query 601 CCCAGTCAGAATCTCAGCCTGAGGACGGTGTTGGCTTCGGCAGCCCCGAGATACATCAGA 660
Sbjct 5601 ............................................................ 5660
Query 661 GGGTGGGCACGCTCCTCCCTCCACTCGCCCCTCAAACAAATGCCCCGCAGCCCATTTCTC 720
Sbjct 5661 ............................................................ 5720
Query 721 CACCCTCATTTGATGACCGCAGATTCAAGTGTTTTGTTAAGTAAAGTCCTGGGTGACCTG 780
Sbjct 5721 ............................................................ 5780
Query 781 GGGTCACAGGGTGCCCCACGCTGCCTGCCTCTGGGCGAACACCCCATCACGCCCGGAGGA 840
Sbjct 5781 ............................................................ 5840
Query 841 GGGCGTGGCTGCCTGCCTGAGTGGGCCAGACCCCTGTCGCCAGGCCTCACGGCAGCTCCA 900
Sbjct 5841 ............................................................ 5900
Query 901 TAGTCAGGAGATGGGGAAGATGCTGGGGACAGGCCCTGGGGAGAAGTACTGGGATCACCT 960
Sbjct 5901 ............................................................ 5960
Query 961 GTTCAGGCTCCCACTGTGACGCTGCCCCGGGGCGGGGGAAGGAGGTGGGACATGTGGGCG 1020
Sbjct 5961 ............................................................ 6020
Query 1021 TTGGGGCCTGTAGGTCCACACCCAGTGTGGGTGACCCTCCCTCTAACCTGGGTCCAGCCC 1080
Sbjct 6021 ............................................................ 6080
Query 1081 GGCTGGAGATGGGTGGGAGTGCGACCTAGGGCTGGCGGGCAGGCGGGCACTGTGTCTCCC 1140
Sbjct 6081 ............................................................ 6140
Query 1141 TGACTGTGTCCTCCTGTGTCCCTCTGCCTCGCCGCTGTTCCGGAACCTGCTCTGCGCGGC 1200
Sbjct 6141 ............................................................ 6200
Query 1201 ACGTCCTGGCAGTGGGGCAGGTGGAGCTGGGCGGGGGCCCTGGTGCAGGCAGCCTGCAGC 1260
Sbjct 6201 ............................................................ 6260
Query 1261 CCTTGGCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCA 1320
Sbjct 6261 ............................................................ 6320
Query 1321 TCTGCTCCCTCTACCAGCTGGAGAACTACTGCAACTAGACGCAGCCCGCAGGCAGCCCCA 1380
Sbjct 6321 ............................................................ 6380
Query 1381 CACCCGCCGCCTCCTGCACCGAGAGAGATGGAATAAAGCCCTTGAACCAGC 1431
Sbjct 6381 ................................................... 6431
Query: NC_000011.10:c2161209-2159779 Homo sapiens chromosome 11, GRCh38.p14 Primary Assembly Query ID: lcl|Query_43125 Length: 1431
Parte2 comparação com o sequenciamento do homo sapiens
>PREDICTED: Theropithecus gelada insulin (INS), transcript variant X2, mRNA
Sequence ID: XM_025355226.1 Length: 651
Range 1: 2 to 427
Score:568 bits(307), Expect:1e-156, 
Identities:389/426(91%), Gaps:16/426(3%), Strand: Plus/Plus
Query 10 GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTGTTCCAAGGGCCTTTGCGTCAGG 69
Sbjct 2 .........................G.........................C........ 61
Query 70 TGGGCTCAGG--------A--------TTCCAGGGTGGCTGGACCCCAGGCCCCAGCTCT 113
Sbjct 62 ..........GCTGCCCC.CTTGGGGG................................. 121
Query 114 GCAGCAGGGAGGACGTGGCTGGGCTCGTGAAGCATGTGGGGGTGAGCCCAGGGGCCCCAA 173
Sbjct 122 ...A..........A...........T......G.T.......................G 181
Query 174 GGCAGGGCACCTGGCCTTCAGCCTGCCTCAGCCCTGCCTGTCTCCCAGATCACTGTCCTT 233
Sbjct 182 .......................G.......G................G........... 241
Query 234 CTGCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGAC 293
Sbjct 242 .C.......................................................... 301
Query 294 CTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTC 353
Sbjct 302 .....T.G.T.C.G..............G..............C................ 361
Query 354 TCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAG 413
Sbjct 362 .......G...........G........................................ 421
Query 414 AGGACC 419
Sbjct 422 ...... 427
Range 2: 433 to 651
Score:316 bits(171), Expect:4e-81, 
Identities:204/220(93%), Gaps:1/220(0%), Strand: Plus/Plus
Query 1212 GTGGGGCAGGTGGAGCTGGGCGGGGGCCCTGGTGCAGGCAGCCTGCAGCCCTTGGCCCTG 1271
Sbjct 433 ................................C.......................G... 492
Query 1272 GAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTC 1331
Sbjct 493 ....................C.....C.....G..G.....C.................G 552
Query 1332 TACCAGCTGGAGAACTACTGCAACTAGACGCAGCCCGCAGGCAGCCCCACACCCGCCGCC 1391
Sbjct 553 ............................T..G..........G.-.........T..A.. 611
Query 1392 TCCTGCACCGAGAGAGATGGAATAAAGCCCTTGAACCAGC 1431
Sbjct 612 .........A........C..................... 651
Parte 3 analise filogenética
 
Parte 5 – visualização da proteína em 3D
Parte 6 – vias metabólicas KEEG
4- Conclusão
Bibliografia
https://www.ebi.ac.uk/Tools/msa/clustalo/
https://www.ncbi.nlm.nih.gov/
https://blast.ncbi.nlm.nih.gov/Blast.cgi
https://www.uniprot.org/
https://www.genome.jp/kegg/
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