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DETOXONDRIA
Detoxifi cation - Oxygenation - Tired Skin
DETOXONDRIA…
… TO REACTIVATE THE WASTE RECYCLING MECHANISM.
The vitality, functioning and ageing of cells depends on mitochondrial homoeostasis. Every day, this
homoeostasis is threatened. Respiration produces toxic free radicals which damage the component parts 
of the mitochondria. This generates waste which builds up and interferes with the functioning of this vital 
organ, endangering the life of the cell itself. 
… TO PROMOTE DETOXIFICATION AND COMBAT CELLULAR SENESCENCE.
When the accumulation of damage to the mitochondria reaches the point of no return, the cell initiates a 
survival refl ex to recycle the damaged mitochondria: MITOPHAGY. With ageing, and as they lose the ability 
to activate mitophagy, non-functional mitochondria build up and the cells enter a senescent phase. 
… TO OXYGENATE DULL SKIN AND ELIMINATE SIGNS OF FATIGUE.
By reactivating the mitochondrial waste recycling mechanism and the mitophagic performance of the cells, 
Detoxondria improves tissue oxygenation and luminosity of the skin. It also reduces the susceptibility of 
the skin to fatigue and signs of fatigue.
ORIGIN AND CHARACTERISTICS
… A MICRO ALGAE EXPERT AT SURVIVING OXIDATIVE STRESS.
To address the problems of cellular senescence, we have developed a biotechnology extract from Rhodella violacea.
Isolated in Germany in 1951 by Kornmann, this violet-red micro algae possesses very high resistance to oxidative 
stress due to a pool of catalases and peroxydases. This enzymatic pool enables it to recycle H2O2 and quickly reduce 
the concentration of this highly oxidising compound from the environment in which it is growing. Its resistance to 
oxidative stress is also displayed by secretion of a unique exopolysaccharide which forms a protective mucilage around 
its cells. 
… AND GROWN USING A HOSTILE CULTURE MODEL.
In order to stimulate its ability to resist stress, we cultivate Rhodella in photobioreactors 500 - 700 litres in size where 
it is repeatedly exposed to severe oxidative stress using hydrogen peroxide.
In addition to stimulating its intra-cellular defences, Rhodella reacts by producing a protective exopolysaccharide.
Special concentration methods are the used to produce a concentrate of exopolysaccharides and intracellular 
enzymes: Detoxondria. 
The vitality, functioning and ageing of cells depends on 
mitochondrial homoeostasis.
 
Codif TN Laboratories have never ceased studying the 
central role of the mitochondria in cutaneous cells.
Our work has led to 
4 publications
4 posters
1 Innovation Prize
For the fi rst time in cosmetics, Codif Laboratories 
has described a method for determining cellular
senescence based on 3 cellular variables: cell size, 
mitochondrial mass, autophagy; and has defi ned a 
MITOPHAGIC PERFORMANCE INDEX.
Fibroblasts from donor
of 6 years
Fibroblasts from donor
of 70 years
Fibroblasts from donor
of 30 years
0,3
0,25
0,2
0,15
0,1
0,05
0
M
ito
ph
ag
ic
 P
er
fo
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an
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 In
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x
Variation in Mitophagic Performance Index as a function of age and senescence
Before senescence After senescence
 MECHANISM OF ACTION
As they lose the ability to activate mitophagy, non-functional mitochondria build up and the cells enter a senescent 
phase. A decrease in mitophagic performance can therefore be used to indicate cellular senescence.
 CODIF LABORATORIES HAS DEFINED A MITOPHAGIC PERFORMANCE INDEX
 The calculation is based on an analysis of 3 markers for cellular senescence.
 DETOXONDRIA ACTS ON:
 1 - Activation of mitochondrial detoxification processes.
 2 - Improvement in the mitochondrial network.
 3 - Improvement in Mitophagic Performance Index.
WITH BENEFITS FOR
 • Oxygenation of the skin.
 • Susceptibility of the skin to fatigue. 
 • Luminosity of the skin tone.
 • Signs of fatigue.
 mitochondrial mass / autophagy 
 cellular size
x 1000( )
Before senescence
Before senescence
Without DETOXONDRIA With DETOXONDRIA
After senescence
Network highly ramified
Senescence + Detoxondria
Return to a non-ramified network
After senescence
0,25
0,2
0,15
0,1
0,05
0
0,26
0,24
0,22
0,2
0,18
0,16
0,14
0,12
0,1
0,08
0,06
0,04
0,02
0
0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75
M
ito
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ag
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er
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an
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 In
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x
M
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 P
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an
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 In
de
x
Age of fibroblasts
Variation Mitophagic Performance Index 
Mitophagic Performance Index as a 
function of fibroblast age
+137% *** expression of LON protein in mitochondria
Below: Lon mitochondrial in yellow orange.
Improvement in mitochondrial network: -31% fewer branch 
connections
Below: marking of mitochondria in orange and nuclei in blue.
Without Detoxondria
With Detoxondria
+ 14 %
+ 21 %
***p<0.001 Student t test
MPI of
0.197
=
Fibroblasts 
of
28 years
MPI of
0.172
=
Fibroblasts 
of
42 years
IN VITRO EFFICACY
Detoxondria activates the processes of mitochondrial detoxification: +137% *** Lon protein
Lon is a protein which fulfils several functions for mitochondrial homoeostasis. Associated with chaperone 
molecules, it maintains the correct conformation of protein molecules. Protease activity: it is able to break down 
damaged proteins so they can be recycled. Associated with mitochondrial DNA, it is involved in the biogenesis of 
new mitochondria.
Detoxondria improves the mitochondrial network: 31% fewer ramifications
As waste builds up in the mitochondria, the cells accumulate non-functional mitochondria. Before activating 
mitophagy the cells adopt an intermediate strategy. They fuse the defective mitochondria with functional 
mitochondria. This fusion strategy leads to the creation of a mitochondrial network with a more branch-like structure 
which is characteristic of senescent cells.
Detoxondria improves the Mitophagic Performance Index: up to 21% increase
The increase in the mitophagic performance index shows an improvement in overall cellular detoxification whatever 
the age of the cells or their degree of senescence. 
Detoxondria reverses cellular ageing by 14 years
The work carried out on our panel of fibroblasts enables us to associate a Mitophagic Performance Index (MPI) 
with a cellular age. Fibroblasts with a cellular age of 42 years attain a cellular age of 28 years after treatment with 
Detoxondria, i.e. cellular rejuvenation of 14 years.
Detoxondria improves oxygenation of the skin
Detoxondria reduces susceptibility of the skin 
to fatigue
Detoxondria eliminates dull complexion
Detoxondria reduces signs of fatigue
*p<0.05 ; **p<0.01 Wilcoxon test ; ++p<0.01; +++p<0.001 Student t test/ ls = significance limit
After 24H After 28 days
Hysteresis Residual deformation Creased skin Drawn lines Shadows Bags
Luminosity 
of the skin
Fineness 
in skin grain
Freshness of 
complexion
+ 6 %
+ 10 %
+ 8 %
+ 17 %**
+ 27 %*
- 11 %++
- 2 %
- 5 % ls
- 6 % ls
- 17 % ls
- 15 %+++
12
10
8
6
4
2
0
30
25
20
15
10
5
0
0
-2
-4
-6
-8
-10
-12
-14
-16
0
-2
-4
-6
-8
-10
-12
-14
-16
-18
Variation in Transcutaneous O2 partial pressure versus placebo
Va
ria
tio
n 
in
 T
cP
O
2 
in
 %
%
 v
ar
ia
tio
n 
ve
rs
us
 p
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%
 V
ar
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ve
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us
 p
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%
 v
ar
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tio
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ve
rs
us
 T
0
Improvement in complexion versus placebo 
 after 28 days application
Variation in susceptibility to fatigue parameters of the skin Variations in visible signs of fatigue versus placebo
IN VIVO EFFICACYDetoxondria eliminates dull complexion
Detoxondria reduces signs of fatigue
Tested on two panels of 20 and 30 volunteers aged 50 to 65 years of age.
2 applications per day for 28 days on the entire face.
Application of Detoxondria at 0.75% or a placebo.
Detoxondria increases oxygenation of the skin after 24 hours
 +6% on average (versus placebo) after 24 hrs and up to +126% 
 +10% on average (versus placebo) after 28 days and upto +60%
Detoxondria improves luminosity of skintone after 28 days
 Luminosity of the skin: +8% on average (versus placebo) and up to +100%
 Freshness of the skin: +17% ** on average (versus placebo) and up to +100%
 Fineness of the grain of the skin: +27% * on average (versus placebo) and up to +400%
Detoxondria reduces susceptibility of the skin to fatigue after 28 days
Measurement of cutaneous elasticity is based on the suction method. This is repeated 10 times to “fatigue” the skin. 
The two parameters measured to assess the susceptibility of the skin to fatigue (elasticity) are the amplitude of 
deformation of the skin after 10 suctions (hysteresis, H) and residual deformation of the skin after suction, R. 
 -11%++ reduction on average for hysteresis and up to 66% reduction
 -15% +++ on average for residual deformation and up to -33%
Detoxondria reduces signs of fatigue after 28 days
 Creased appearance of the skin: -2% on average (versus placebo) and up to -33%.
 Drawn Lines: -5% on average (versus placebo) and up to -30%.
 Shadows: -6% on average (versus placebo) and up to -100%.
 Bags under the eyes: -17% on average (versus placebo) and up to -67%
VISIBLE BENEFITS ON THE LUMINOSITY OF THE SKIN
T0
T0
T28
T28
CARE BENEFITS
OXYGENATING
DETOXIFYING
SUSCEPTIBILITY TO FATIGUE
DULL COMPLEXION
DETOXONDRIA PROMOTES DETOXIFICATION
 Increase in LON synthesis.
 Increase in Mitophagic Performance Index.
 Improved oxygenation of the skin.
DETOXONDRIA REJUVENATES THE SKIN
 Improvement in mitochondrial network.
 Rejuvenating eff ect on senescent cells.
 Improvement in luminosity, grain and freshness of the skin.
DETOXONDRIA REVIVES FATIGUED SKIN
 Reduced cutaneous susceptibility to fatigue.
 Reduced creased eff ect, drawn lines, shadows and bags.
Phase Raw ingredient / Trade name INCI Name %
A
DEMINERALISED WATER Water 64.35
ELESTAB CPN (1) Chlorphenesin 0.27
EDETA BD (1) Disodium edta 0.10
GLYCERINE BIDISTILLEE CODEX (2) Glycerin 5.00
B ARISTOFLEX AVC (3) Ammonium acryloyldimethyltaurate/vp copolymer 1.00
C
SP BRIJ S2 MBAL-PA-RB (4) Steareth-2 3.00
BRIJ 721P (2) Steareth-21 2.00
ALCOOL CETYLIQUE / LANETTE 16 (1) Cetyl alcohol 2.20
SOFTISAN 100 (5) Hydrogenated coco-glycerides 2.00
LIPOCIRE A PASTILLES (6) C10-18 triglycerides 1.50
EUTANOL G (1) Octyldodecanol 7,30
LANOL 99 (7) Isononyl isononanoate 7.70
PHENOXYETHANOL (8) Phenoxyethanol 0,80
SILICONE (DIMETHICONE (100CS)) (9) Dimethicone 1.00
D SOLUTION DE SOUDE SOUDE 6.25N (10) Aqua & Sodium hydroxide 0.03
E
COVI-OX T90EU C (1) Tocopherol & Helianthus annuus seed oil 0.05
FRAGRANCE Fragrance 0.20
DETOXONDRIA (11) Maris aqua & Glycerin & Propanediol & Hydrolyzed rhodophyceae extract 1.50
(1) A.M.I ; (2) QUIMASSO FRANCE EURL ; (3) CLARIANT ; (4) CRODA France ; (5) IMCD; (6) GATTEFOSSE FRANCE SA (7) SEPPIC ; (8) LEVHOSS ; (9) QUIMDIS SA ; (10) BRENNTAG MAINE BRETAGNE ; 
(11) CODIF Technologie Naturelle
OPERATING METHOD: 
• Heat A to 75°C under emulsifier with gentle stirring. • Add B under emulsifier 2500rpm for 10min. • Heat C to 75°C. • Emulsify C into AB under 
emulsifier 2500rpm for 5min. • Add D under emulsifier 2500rpm for 10min. • Cool down to 35°C under gentle stirring. • Add E under gentle stirring for 
20min. • Check the pH.
INDICATIVE FORMULATION • DETOX BOOSTER CREAM
HOW TO USE IT
Water soluble. To be used at 0.75% in:
Water (and) Sea salt (and) Hydrolyzed Rhodophyceae extract (and) Phenethyl alcohol.
In a revitalising booster serum with EARLY BOOST.
In a general anti-ageing cream with PHYCOJUVENINE.
In a skin detoxifi cation treatment with ACTIPORINE 8G.
DETOXONDRIA PA
INCI
infotech@codif-tn.com
commercial@codif-tn.com
70, rue du Commandant l’Herminier - CS 11781 - 35417 Saint-Malo CEDEX - FRANCE
Tel : +33-2-23-18-31-07 - Fax : +33-2-23-18-31-01
www.codif-tn.com
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