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<p>Questions</p><p>1. What are the samples?</p><p>Answer: They are saliva samples.</p><p>2. How many samples in total?</p><p>Answer: 4 types of ramps * 2 body weight * 3 pigs * 6 replications * 2 sampling time = 288 samples.</p><p>3. Different types? treatment? Are they divided into specific situations?</p><p>Answer: 72 samples/type of ramp and 144/body weight.</p><p>RAMP = 4 treats (A000, A15H, A15R, A25z)</p><p>WEIGHT = 2 weights (Heavy and LIGHT)</p><p>SAMPLING TIME = 2 sampling time (PRE and POST)</p><p>4. Where are they (which temperature)?</p><p>Answer: -80◦C (ultra-freezer).</p><p>5. How will you divide them into plates for analyses?</p><p>1</p><p>2</p><p>3</p><p>4</p><p>5</p><p>6</p><p>7</p><p>8</p><p>9</p><p>10</p><p>11</p><p>12</p><p>1</p><p>Std</p><p>Std</p><p>C1</p><p>C1</p><p>S7</p><p>S7</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>2</p><p>Std</p><p>Std</p><p>C2</p><p>C2</p><p>S8</p><p>S8</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>3</p><p>Std</p><p>Std</p><p>S1</p><p>S1</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>4</p><p>Std</p><p>Std</p><p>S2</p><p>S2</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>5</p><p>Std</p><p>Std</p><p>S3</p><p>S3</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>6</p><p>Std</p><p>Std</p><p>S4</p><p>S4</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>7</p><p>0</p><p>0</p><p>S5</p><p>S5</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>8</p><p>NSB</p><p>NSB</p><p>S6</p><p>S6</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Sx</p><p>Std = Standard; NSB = non-specific binding wells; C = controls; S = samples of each treatment.</p><p>Answer:</p><p>· 12 * 8 = 96 well</p><p>· Samples = 38 (96 wells -20 wells used as std and control = 76 wells available for the samples, 2 DUPLICATES par sample => 76/2 = 38 samples)</p><p>· ALL TREATMENTS SHOULD BE PRESENT IN A PLATE = 4 types of ramps * 2 body weight = 8 pigs</p><p>· 8 pigs * 2 DIFFERENT REPLICATES = 16 PIGS</p><p>· 16 PIGS * 2 sampling time = 32 samples</p><p>6. How many plates do you need?</p><p>Answer:</p><p>· 288 samples in total/32 samples = 9 plates ≅ 10 plates</p><p>7. How many samples are you defrosting/analyse?</p><p>You are going to defrost 32 SAMPLES coming from 16 PIGS (8 PIGS from one REP and 8 PIGS from another REP).</p><p>8PIGS = 1 pig heavy A000, 1 pig Light A000, 1 pig heavy A15H, 1 pig Light A15H, 1 pig heavy A15R, 1 pig Light A15R, 1 pig heavy A25z, 1 pig Light A25z</p><p>Answer:</p><p>· ≅ 45 minutes - 1 h on ice.</p>